LOCUS       BBa_K195617             1450 bp    DNA     linear       22-Dec-2024
DEFINITION  pTet with activator gene CII  and pRE with GFP generator
ACCESSION   BBa_K195617
VERSION     BBa_K195617.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..374
                     /label=BBa_K116602
     stem_loop       394..437
                     /label=stem_loop
     misc_feature    383..462
                     /label=BBa_B0010
     terminator      383..462
                     /label=BBa_B0010
     stem_loop       478..497
                     /label=T7 TE
     misc_feature    504..504
                     /label=stop
     polyA_site      498..511
                     /label=polya
     misc_feature    471..511
                     /label=BBa_B0012
     terminator      471..511
                     /label=BBa_B0012
     promoter        520..567
                     /label=BBa_K116603
     misc_feature    580..583
                     /label=conserved
     RBS             576..587
                     /label=BBa_B0034
     CDS             594..1313
                     /label=GFP protein
     CDS             594..1313
                     /label=BBa_E0040
     stem_loop       1333..1376
                     /label=stem_loop
     misc_feature    1322..1401
                     /label=BBa_B0010
     terminator      1322..1401
                     /label=BBa_B0010
     stem_loop       1417..1436
                     /label=T7 TE
     misc_feature    1443..1443
                     /label=stop
     polyA_site      1437..1450
                     /label=polya
     misc_feature    1410..1450
                     /label=BBa_B0012
     terminator      1410..1450
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggttcgtg caaacaaacg caacgaggct ctacgaatcg
      121 agagtgcgtt gcttaacaaa atcgcaatgc ttggaactga gaagacagcg gaagctgtgg
      181 gcgttgataa gtcgcagatc agcaggtgga agagggactg gattccaaag ttctcaatgc
      241 tgcttgctgt tcttgaatgg ggggtcgttg acgacgacat ggctcgattg gcgcgacaag
      301 ttgctgcgat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      361 agatggagtt ctgatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      421 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      481 tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagcctcgtt gcgtttgttt
      541 gcacgaacca tatgtaagta tttcctttac tagagaaaga ggagaaatac tagatgcgta
      601 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      661 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      721 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      781 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
      841 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
      901 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      961 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
     1021 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
     1081 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     1141 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     1201 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     1261 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga
     1321 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1381 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1441 tgcgtttata
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