##gff-version 3
##sequence-region BBa_K195617 1 1450
BBa_K195617	.	promoter	520	567	.	+	0	ID=BBa_K116603;Name=BBa_K116603
BBa_K195617	.	ribosome_entry_site	576	587	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K195617	.	conserved_region	580	583	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K195617	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K195617	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K195617	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K195617	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K195617	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K195617	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K195617	.	CDS	594	1313	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K195617	.	CDS	594	1313	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K195617	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K195617	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K195617	.	CDS	81	374	.	+	0	ID=BBa_K116602;Name=BBa_K116602
BBa_K195617	.	terminator	471	511	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K195617	.	polyA_site	498	511	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K195617	.	stem_loop	478	497	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K195617	.	stop_codon	504	504	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K195617	.	engineered_region	471	511	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K195617	.	terminator	1322	1401	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K195617	.	stem_loop	1333	1376	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K195617	.	engineered_region	1322	1401	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K195617	.	terminator	1410	1450	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K195617	.	polyA_site	1437	1450	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K195617	.	stem_loop	1417	1436	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K195617	.	stop_codon	1443	1443	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K195617	.	engineered_region	1410	1450	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K195617	.	terminator	383	462	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K195617	.	stem_loop	394	437	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K195617	.	engineered_region	383	462	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K195617
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaact
gagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaa
tgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcac
caataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgatactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagagagcctcgttgcgtttgtttgcacgaaccatat
gtaagtatttcctttactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtccca
attcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacg
gaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtta
tggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttat
gtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccc
ttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaacta
taactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacatt
gaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagaca
accattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgt
aacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttata