LOCUS       BBa_K195618             1473 bp    DNA     linear       22-Nov-2024
DEFINITION  pPenI with activator gene CII  and pRE with GFP generator
ACCESSION   BBa_K195618
VERSION     BBa_K195618.1  
FEATURES             Location/Qualifiers
     misc_feature    1..77
                     /label=PenI
     promoter        23..28
                     /label=-35
     promoter        46..51
                     /label=-10
     misc_feature    31..53
                     /label=dimer left half
     misc_feature    55..76
                     /label=dimer right half
     promoter        1..77
                     /label=BBa_R0074
     misc_feature    90..93
                     /label=conserved
     RBS             86..97
                     /label=BBa_B0034
     CDS             104..397
                     /label=BBa_K116602
     stem_loop       417..460
                     /label=stem_loop
     misc_feature    406..485
                     /label=BBa_B0010
     terminator      406..485
                     /label=BBa_B0010
     stem_loop       501..520
                     /label=T7 TE
     misc_feature    527..527
                     /label=stop
     polyA_site      521..534
                     /label=polya
     misc_feature    494..534
                     /label=BBa_B0012
     terminator      494..534
                     /label=BBa_B0012
     promoter        543..590
                     /label=BBa_K116603
     misc_feature    603..606
                     /label=conserved
     RBS             599..610
                     /label=BBa_B0034
     CDS             617..1336
                     /label=GFP protein
     CDS             617..1336
                     /label=BBa_E0040
     stem_loop       1356..1399
                     /label=stem_loop
     misc_feature    1345..1424
                     /label=BBa_B0010
     terminator      1345..1424
                     /label=BBa_B0010
     stem_loop       1440..1459
                     /label=T7 TE
     misc_feature    1466..1466
                     /label=stop
     polyA_site      1460..1473
                     /label=polya
     misc_feature    1433..1473
                     /label=BBa_B0012
     terminator      1433..1473
                     /label=BBa_B0012
ORIGIN
        1 tcatttccaa ccgaaaaaag gcttgcattt aaatcttaca tatgtaatac tttcaaagac
       61 tacatttgta agatttgtac tagagaaaga ggagaaatac tagatggttc gtgcaaacaa
      121 acgcaacgag gctctacgaa tcgagagtgc gttgcttaac aaaatcgcaa tgcttggaac
      181 tgagaagaca gcggaagctg tgggcgttga taagtcgcag atcagcaggt ggaagaggga
      241 ctggattcca aagttctcaa tgctgcttgc tgttcttgaa tggggggtcg ttgacgacga
      301 catggctcga ttggcgcgac aagttgctgc gattctcacc aataaaaaac gcccggcggc
      361 aaccgagcgt tctgaacaaa tccagatgga gttctgatac tagagccagg catcaaataa
      421 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      481 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
      541 agagagcctc gttgcgtttg tttgcacgaa ccatatgtaa gtatttcctt tactagagaa
      601 agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
      661 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      721 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      781 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
      841 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      901 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
      961 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     1021 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     1081 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     1141 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     1201 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     1261 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     1321 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1381 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1441 gctcaccttc gggtgggcct ttctgcgttt ata
//