LOCUS       BBa_K195619             1596 bp    DNA     linear       29-Nov-2024
DEFINITION  pLacI with activator gene CII  and pRE with GFP generator
ACCESSION   BBa_K195619
VERSION     BBa_K195619.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..520
                     /label=BBa_K116602
     stem_loop       540..583
                     /label=stem_loop
     misc_feature    529..608
                     /label=BBa_B0010
     terminator      529..608
                     /label=BBa_B0010
     stem_loop       624..643
                     /label=T7 TE
     misc_feature    650..650
                     /label=stop
     polyA_site      644..657
                     /label=polya
     misc_feature    617..657
                     /label=BBa_B0012
     terminator      617..657
                     /label=BBa_B0012
     promoter        666..713
                     /label=BBa_K116603
     misc_feature    726..729
                     /label=conserved
     RBS             722..733
                     /label=BBa_B0034
     CDS             740..1459
                     /label=GFP protein
     CDS             740..1459
                     /label=BBa_E0040
     stem_loop       1479..1522
                     /label=stem_loop
     misc_feature    1468..1547
                     /label=BBa_B0010
     terminator      1468..1547
                     /label=BBa_B0010
     stem_loop       1563..1582
                     /label=T7 TE
     misc_feature    1589..1589
                     /label=stop
     polyA_site      1583..1596
                     /label=polya
     misc_feature    1556..1596
                     /label=BBa_B0012
     terminator      1556..1596
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ttcgtgcaaa
      241 caaacgcaac gaggctctac gaatcgagag tgcgttgctt aacaaaatcg caatgcttgg
      301 aactgagaag acagcggaag ctgtgggcgt tgataagtcg cagatcagca ggtggaagag
      361 ggactggatt ccaaagttct caatgctgct tgctgttctt gaatgggggg tcgttgacga
      421 cgacatggct cgattggcgc gacaagttgc tgcgattctc accaataaaa aacgcccggc
      481 ggcaaccgag cgttctgaac aaatccagat ggagttctga tactagagcc aggcatcaaa
      541 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      601 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
      661 tagagagagc ctcgttgcgt ttgtttgcac gaaccatatg taagtatttc ctttactaga
      721 gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      781 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      841 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      901 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      961 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1021 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1081 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1141 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1201 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     1261 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1321 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     1381 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     1441 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1501 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1561 ctggctcacc ttcgggtggg cctttctgcg tttata
//