BBa_K195619
type: ComponentDefinition
persistent identity: BBa K195619
display id: BBa_K195619
version: 1
was generated by: iGEM to SBOL conversion
description: pLacI with activator gene CII and pRE with GFP generator
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:17Z
mutable provenance: biobrick
owned by: james
owned by: myers
mutable description: This part is composed of pLac with inducer gene CII, and it would induce pRE to produce green fluorescent protein. The coding sequence CII could induce BBa_K116603,the regulatory promoter pRE from λ phage. The pLac is repressed by LacI, which could be inhibited by IPTG.The pRE would be induced by CII produced by the downstream gene of the regulatory promoter pLac and produce green fluorescent protein.
discontinued: false
dominant: false
experience: experience/Works
group u list: _292_
m user id: 0
owner id: 4841
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Yun-Hsiang Chang
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_E0040
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_K116602
component: BBa_K116603
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K116602
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K116603
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K195619_sequence/1

BBa_E0040
type: Component
persistent identity: component2050797
display id: component2050797
version: 1
top level: BBa_K195619
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component2050795
display id: component2050795
version: 1
top level: BBa_K195619
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2050800
display id: component2050800
version: 1
top level: BBa_K195619
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2050798
display id: component2050798
version: 1
top level: BBa_K195619
definition: BBa_B0010
access: public

BBa_B0010
type: Component
persistent identity: component2050787
display id: component2050787
version: 1
top level: BBa_K195619
definition: BBa_B0010
access: public

BBa_K116602
type: Component
persistent identity: component2050786
display id: component2050786
version: 1
top level: BBa_K195619
definition: CII
access: public

BBa_K116603
type: Component
persistent identity: component2050793
display id: component2050793
version: 1
top level: BBa_K195619
definition: pRE
access: public

BBa_B0012
type: Component
persistent identity: component2050789
display id: component2050789
version: 1
top level: BBa_K195619
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component2050785
display id: component2050785
version: 1
top level: BBa_K195619
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2050777
display id: component2050777
version: 1
top level: BBa_K195619
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2050777
display id: annotation2050777
version: 1
top level: BBa_K195619
location: https://synbiohub.org/public/igem/BBa_K195619/annotation2050777/range2050777/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K195619/annotation2050777/range2050777/1
type: Range
persistent identity: range2050777
display id: range2050777
version: 1
top level: BBa_K195619
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2050785
display id: annotation2050785
version: 1
top level: BBa_K195619
location: https://synbiohub.org/public/igem/BBa_K195619/annotation2050785/range2050785/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K195619/annotation2050785/range2050785/1
type: Range
persistent identity: range2050785
display id: range2050785
version: 1
top level: BBa_K195619
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_K116602
type: SequenceAnnotation
persistent identity: annotation2050786
display id: annotation2050786
version: 1
top level: BBa_K195619
location: https://synbiohub.org/public/igem/BBa_K195619/annotation2050786/range2050786/1
component: BBa_K116602

https://synbiohub.org/public/igem/BBa_K195619/annotation2050786/range2050786/1
type: Range
persistent identity: range2050786
display id: range2050786
version: 1
top level: BBa_K195619
direction: direction/reverse
start: 227
end: 520
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2050787
display id: annotation2050787
version: 1
top level: BBa_K195619
location: https://synbiohub.org/public/igem/BBa_K195619/annotation2050787/range2050787/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K195619/annotation2050787/range2050787/1
type: Range
persistent identity: range2050787
display id: range2050787
version: 1
top level: BBa_K195619
direction: direction/forward
start: 529
end: 608
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2050789
display id: annotation2050789
version: 1
top level: BBa_K195619
location: https://synbiohub.org/public/igem/BBa_K195619/annotation2050789/range2050789/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K195619/annotation2050789/range2050789/1
type: Range
persistent identity: range2050789
display id: range2050789
version: 1
top level: BBa_K195619
direction: direction/forward
start: 617
end: 657
orientation: inline

BBa_K116603
type: SequenceAnnotation
persistent identity: annotation2050793
display id: annotation2050793
version: 1
top level: BBa_K195619
location: https://synbiohub.org/public/igem/BBa_K195619/annotation2050793/range2050793/1
component: BBa_K116603

https://synbiohub.org/public/igem/BBa_K195619/annotation2050793/range2050793/1
type: Range
persistent identity: range2050793
display id: range2050793
version: 1
top level: BBa_K195619
direction: direction/reverse
start: 666
end: 713
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2050795
display id: annotation2050795
version: 1
top level: BBa_K195619
location: https://synbiohub.org/public/igem/BBa_K195619/annotation2050795/range2050795/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K195619/annotation2050795/range2050795/1
type: Range
persistent identity: range2050795
display id: range2050795
version: 1
top level: BBa_K195619
direction: direction/reverse
start: 722
end: 733
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2050797
display id: annotation2050797
version: 1
top level: BBa_K195619
location: https://synbiohub.org/public/igem/BBa_K195619/annotation2050797/range2050797/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K195619/annotation2050797/range2050797/1
type: Range
persistent identity: range2050797
display id: range2050797
version: 1
top level: BBa_K195619
direction: direction/reverse
start: 740
end: 1459
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2050798
display id: annotation2050798
version: 1
top level: BBa_K195619
location: https://synbiohub.org/public/igem/BBa_K195619/annotation2050798/range2050798/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K195619/annotation2050798/range2050798/1
type: Range
persistent identity: range2050798
display id: range2050798
version: 1
top level: BBa_K195619
direction: direction/forward
start: 1468
end: 1547
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2050800
display id: annotation2050800
version: 1
top level: BBa_K195619
location: https://synbiohub.org/public/igem/BBa_K195619/annotation2050800/range2050800/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K195619/annotation2050800/range2050800/1
type: Range
persistent identity: range2050800
display id: range2050800
version: 1
top level: BBa_K195619
direction: direction/forward
start: 1556
end: 1596
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

CII
type: ComponentDefinition
persistent identity: BBa K116602
display id: BBa_K116602
version: 1
was generated by: iGEM to SBOL conversion
description: CII coding region from λ phage
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: λ phage
owned by: james
owned by: myers
mutable description: The CII coding region from λ phage.
discontinued: false
dominant: true
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none.
star: false
creator: Jesse Wu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K116602_sequence/1

pRE
type: ComponentDefinition
persistent identity: BBa K116603
display id: BBa_K116603
version: 1
was generated by: iGEM to SBOL conversion
description: pRE promoter from λ phage
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: λ phage
owned by: james
owned by: myers
mutable description: The pRE coding region from λ phage. It is able to be induced by CII (BBa_K116602).
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none.
star: false
creator: Jesse Wu
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K116603_sequence/1

https://synbiohub.org/public/igem/BBa_K195619_sequence/1
type: Sequence
persistent identity: BBa K195619 sequence
display id: BBa_K195619_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctttactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116602_sequence/1
type: Sequence
persistent identity: BBa K116602 sequence
display id: BBa_K116602_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116603_sequence/1
type: Sequence
persistent identity: BBa K116603 sequence
display id: BBa_K116603_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z