LOCUS       BBa_K195620              896 bp    DNA     linear       02-May-2025
DEFINITION  pRE with repressor gene TetR
ACCESSION   BBa_K195620
VERSION     BBa_K195620.1  
FEATURES             Location/Qualifiers
     promoter        1..48
                     /label=BBa_K116603
     misc_feature    61..64
                     /label=conserved
     RBS             57..68
                     /label=BBa_B0034
     CDS             78..694
                     /label=tetR
     misc_feature    695..728
                     /label=SsrA
     misc_feature    735..759
                     /label=Help:Barcodes
     CDS             75..759
                     /label=BBa_C0040
     stem_loop       779..822
                     /label=stem_loop
     misc_feature    768..847
                     /label=BBa_B0010
     terminator      768..847
                     /label=BBa_B0010
     stem_loop       863..882
                     /label=T7 TE
     misc_feature    889..889
                     /label=stop
     polyA_site      883..896
                     /label=polya
     misc_feature    856..896
                     /label=BBa_B0012
     terminator      856..896
                     /label=BBa_B0012
     terminator      768..896
                     /label=BBa_B0015
ORIGIN
        1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttta ctagagaaag
       61 aggagaaata ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc
      121 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg
      181 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag
      241 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc
      301 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg
      361 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc
      421 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg
      481 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag
      541 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat
      601 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg
      661 gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt
      721 tagtagctta ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat
      781 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      841 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//