LOCUS       BBa_K195621              659 bp    DNA     linear       30-Jun-2024
DEFINITION  pRE with repressor gene PenI(+LVA)
ACCESSION   BBa_K195621
VERSION     BBa_K195621.1  
FEATURES             Location/Qualifiers
     promoter        1..48
                     /label=BBa_K116603
     misc_feature    61..64
                     /label=conserved
     RBS             57..68
                     /label=BBa_B0034
     CDS             75..461
                     /label=penI
     misc_feature    459..491
                     /label=LVA
     misc_feature    498..522
                     /label=Help:Barcodes
     CDS             75..522
                     /label=BBa_C0074
     stem_loop       542..585
                     /label=stem_loop
     misc_feature    531..610
                     /label=BBa_B0010
     terminator      531..610
                     /label=BBa_B0010
     stem_loop       626..645
                     /label=T7 TE
     misc_feature    652..652
                     /label=stop
     polyA_site      646..659
                     /label=polya
     misc_feature    619..659
                     /label=BBa_B0012
     terminator      619..659
                     /label=BBa_B0012
     terminator      531..659
                     /label=BBa_B0015
ORIGIN
        1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttta ctagagaaag
       61 aggagaaata ctagatgaaa aaaataccac agatttctga tgcggaactc gaagtcatga
      121 aagtgatttg gaagcattct tccattaata ctaatgaggt aatcaaagag ctcagtaaaa
      181 cttcaacgtg gagcccaaaa actattcaga ctatgctgct gcgccttatc aaaaaaggcg
      241 ctctcaacca ccataaagaa ggtcgggttt tcgtttacac gcctaatata gacgaatcag
      301 attatataga ggttaagtca cactcatttc tcaaccggtt ttacaatggt acattaaatt
      361 ccatggtact caactttttg gagaatgatc aactgtcggg agaagaaatc aatgaattgt
      421 atcagatact cgaagaacat aagaaccgta agaaggaagc tgcaaacgac gaaaactacg
      481 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag ccaggcatca
      541 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      601 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//