LOCUS       BBa_K195622             2354 bp    DNA     linear       18-Feb-2025
DEFINITION  CII/TetR Damped Oscillator With GFP Reporter (I)
ACCESSION   BBa_K195622
VERSION     BBa_K195622.1  
FEATURES             Location/Qualifiers
     promoter        1..48
                     /label=BBa_K116603
     misc_feature    61..64
                     /label=conserved
     RBS             57..68
                     /label=BBa_B0034
     CDS             78..694
                     /label=tetR
     misc_feature    695..728
                     /label=SsrA
     misc_feature    735..759
                     /label=Help:Barcodes
     CDS             75..759
                     /label=BBa_C0040
     stem_loop       779..822
                     /label=stem_loop
     misc_feature    768..847
                     /label=BBa_B0010
     terminator      768..847
                     /label=BBa_B0010
     stem_loop       863..882
                     /label=T7 TE
     misc_feature    889..889
                     /label=stop
     polyA_site      883..896
                     /label=polya
     misc_feature    856..896
                     /label=BBa_B0012
     terminator      856..896
                     /label=BBa_B0012
     terminator      768..896
                     /label=BBa_B0015
     misc_feature    905..923
                     /label=TetR 1
     misc_feature    905..958
                     /label=BBa_R0040
     promoter        924..929
                     /label=-35
     misc_feature    930..948
                     /label=TetR 2
     promoter        947..952
                     /label=-10
     promoter        905..958
                     /label=BBa_R0040
     misc_feature    971..974
                     /label=conserved
     RBS             967..978
                     /label=BBa_B0034
     CDS             985..1278
                     /label=BBa_K116602
     stem_loop       1298..1341
                     /label=stem_loop
     misc_feature    1287..1366
                     /label=BBa_B0010
     terminator      1287..1366
                     /label=BBa_B0010
     stem_loop       1382..1401
                     /label=T7 TE
     misc_feature    1408..1408
                     /label=stop
     polyA_site      1402..1415
                     /label=polya
     misc_feature    1375..1415
                     /label=BBa_B0012
     terminator      1375..1415
                     /label=BBa_B0012
     terminator      1287..1415
                     /label=BBa_B0015
     promoter        1424..1471
                     /label=BBa_K116603
     misc_feature    1484..1487
                     /label=conserved
     RBS             1480..1491
                     /label=BBa_B0034
     CDS             1498..2217
                     /label=GFP protein
     CDS             1498..2217
                     /label=BBa_E0040
     stem_loop       2237..2280
                     /label=stem_loop
     misc_feature    2226..2305
                     /label=BBa_B0010
     terminator      2226..2305
                     /label=BBa_B0010
     stem_loop       2321..2340
                     /label=T7 TE
     misc_feature    2347..2347
                     /label=stop
     polyA_site      2341..2354
                     /label=polya
     misc_feature    2314..2354
                     /label=BBa_B0012
     terminator      2314..2354
                     /label=BBa_B0012
     terminator      2226..2354
                     /label=BBa_B0015
ORIGIN
        1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttta ctagagaaag
       61 aggagaaata ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc
      121 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg
      181 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag
      241 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc
      301 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg
      361 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc
      421 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg
      481 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag
      541 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat
      601 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg
      661 gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt
      721 tagtagctta ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat
      781 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      841 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
      901 agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta
      961 ctagagaaag aggagaaata ctagatggtt cgtgcaaaca aacgcaacga ggctctacga
     1021 atcgagagtg cgttgcttaa caaaatcgca atgcttggaa ctgagaagac agcggaagct
     1081 gtgggcgttg ataagtcgca gatcagcagg tggaagaggg actggattcc aaagttctca
     1141 atgctgcttg ctgttcttga atggggggtc gttgacgacg acatggctcg attggcgcga
     1201 caagttgctg cgattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     1261 atccagatgg agttctgata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1321 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1381 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagcct cgttgcgttt
     1441 gtttgcacga accatatgta agtatttcct ttactagaga aagaggagaa atactagatg
     1501 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     1561 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     1621 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
     1681 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
     1741 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     1801 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     1861 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     1921 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     1981 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     2041 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     2101 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     2161 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac
     2221 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2281 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     2341 tttctgcgtt tata
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