LOCUS       BBa_K195623             2140 bp    DNA     linear       18-May-2024
DEFINITION  CII/PenI Damped Oscillator With GFP Reporter (II)
ACCESSION   BBa_K195623
VERSION     BBa_K195623.1  
FEATURES             Location/Qualifiers
     promoter        1..48
                     /label=BBa_K116603
     misc_feature    61..64
                     /label=conserved
     RBS             57..68
                     /label=BBa_B0034
     CDS             75..461
                     /label=penI
     misc_feature    459..491
                     /label=LVA
     misc_feature    498..522
                     /label=Help:Barcodes
     CDS             75..522
                     /label=BBa_C0074
     stem_loop       542..585
                     /label=stem_loop
     misc_feature    531..610
                     /label=BBa_B0010
     terminator      531..610
                     /label=BBa_B0010
     stem_loop       626..645
                     /label=T7 TE
     misc_feature    652..652
                     /label=stop
     polyA_site      646..659
                     /label=polya
     misc_feature    619..659
                     /label=BBa_B0012
     terminator      619..659
                     /label=BBa_B0012
     terminator      531..659
                     /label=BBa_B0015
     misc_feature    668..744
                     /label=PenI
     promoter        690..695
                     /label=-35
     promoter        713..718
                     /label=-10
     misc_feature    698..720
                     /label=dimer left half
     misc_feature    722..743
                     /label=dimer right half
     promoter        668..744
                     /label=BBa_R0074
     misc_feature    757..760
                     /label=conserved
     RBS             753..764
                     /label=BBa_B0034
     CDS             771..1064
                     /label=BBa_K116602
     stem_loop       1084..1127
                     /label=stem_loop
     misc_feature    1073..1152
                     /label=BBa_B0010
     terminator      1073..1152
                     /label=BBa_B0010
     stem_loop       1168..1187
                     /label=T7 TE
     misc_feature    1194..1194
                     /label=stop
     polyA_site      1188..1201
                     /label=polya
     misc_feature    1161..1201
                     /label=BBa_B0012
     terminator      1161..1201
                     /label=BBa_B0012
     terminator      1073..1201
                     /label=BBa_B0015
     promoter        1210..1257
                     /label=BBa_K116603
     misc_feature    1270..1273
                     /label=conserved
     RBS             1266..1277
                     /label=BBa_B0034
     CDS             1284..2003
                     /label=GFP protein
     CDS             1284..2003
                     /label=BBa_E0040
     stem_loop       2023..2066
                     /label=stem_loop
     misc_feature    2012..2091
                     /label=BBa_B0010
     terminator      2012..2091
                     /label=BBa_B0010
     stem_loop       2107..2126
                     /label=T7 TE
     misc_feature    2133..2133
                     /label=stop
     polyA_site      2127..2140
                     /label=polya
     misc_feature    2100..2140
                     /label=BBa_B0012
     terminator      2100..2140
                     /label=BBa_B0012
     terminator      2012..2140
                     /label=BBa_B0015
ORIGIN
        1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttta ctagagaaag
       61 aggagaaata ctagatgaaa aaaataccac agatttctga tgcggaactc gaagtcatga
      121 aagtgatttg gaagcattct tccattaata ctaatgaggt aatcaaagag ctcagtaaaa
      181 cttcaacgtg gagcccaaaa actattcaga ctatgctgct gcgccttatc aaaaaaggcg
      241 ctctcaacca ccataaagaa ggtcgggttt tcgtttacac gcctaatata gacgaatcag
      301 attatataga ggttaagtca cactcatttc tcaaccggtt ttacaatggt acattaaatt
      361 ccatggtact caactttttg gagaatgatc aactgtcggg agaagaaatc aatgaattgt
      421 atcagatact cgaagaacat aagaaccgta agaaggaagc tgcaaacgac gaaaactacg
      481 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag ccaggcatca
      541 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      601 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
      661 actagagtca tttccaaccg aaaaaaggct tgcatttaaa tcttacatat gtaatacttt
      721 caaagactac atttgtaaga tttgtactag agaaagagga gaaatactag atggttcgtg
      781 caaacaaacg caacgaggct ctacgaatcg agagtgcgtt gcttaacaaa atcgcaatgc
      841 ttggaactga gaagacagcg gaagctgtgg gcgttgataa gtcgcagatc agcaggtgga
      901 agagggactg gattccaaag ttctcaatgc tgcttgctgt tcttgaatgg ggggtcgttg
      961 acgacgacat ggctcgattg gcgcgacaag ttgctgcgat tctcaccaat aaaaaacgcc
     1021 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgatactag agccaggcat
     1081 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1141 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1201 atactagaga gagcctcgtt gcgtttgttt gcacgaacca tatgtaagta tttcctttac
     1261 tagagaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc
     1321 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     1381 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     1441 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
     1501 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
     1561 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     1621 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     1681 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
     1741 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     1801 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     1861 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     1921 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     1981 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
     2041 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2101 cacactggct caccttcggg tgggcctttc tgcgtttata
//