##gff-version 3
##sequence-region BBa_K195623 1 2140
BBa_K195623	.	terminator	1073	1201	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K195623	.	terminator	1161	1201	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K195623	.	polyA_site	1188	1201	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K195623	.	stem_loop	1168	1187	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K195623	.	stop_codon	1194	1194	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K195623	.	engineered_region	1161	1201	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K195623	.	terminator	1073	1152	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K195623	.	stem_loop	1084	1127	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K195623	.	engineered_region	1073	1152	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K195623	.	terminator	531	659	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K195623	.	terminator	619	659	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K195623	.	polyA_site	646	659	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K195623	.	stem_loop	626	645	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K195623	.	stop_codon	652	652	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K195623	.	engineered_region	619	659	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K195623	.	terminator	531	610	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K195623	.	stem_loop	542	585	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K195623	.	engineered_region	531	610	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K195623	.	promoter	1	48	.	+	0	ID=BBa_K116603;Name=BBa_K116603
BBa_K195623	.	CDS	75	522	.	+	0	ID=BBa_C0074;Name=BBa_C0074
BBa_K195623	.	CDS	75	461	.	+	0	ID=annotation302638;Name=penI;Parent=BBa_C0074
BBa_K195623	.	tag	459	491	.	+	0	ID=annotation306563;Name=LVA;Parent=BBa_C0074
BBa_K195623	.	engineered_tag	498	522	.	+	0	ID=annotation2214009;Name=Help:Barcodes;Parent=BBa_C0074
BBa_K195623	.	promoter	1210	1257	.	+	0	ID=BBa_K116603;Name=BBa_K116603
BBa_K195623	.	CDS	1284	2003	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K195623	.	CDS	1284	2003	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K195623	.	ribosome_entry_site	753	764	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K195623	.	conserved_region	757	760	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K195623	.	promoter	668	744	.	+	0	ID=BBa_R0074;Name=BBa_R0074
BBa_K195623	.	promoter	690	695	.	+	0	ID=annotation319211;Name=-35;Parent=BBa_R0074
BBa_K195623	.	operator	668	744	.	+	0	ID=annotation302713;Name=PenI;Parent=BBa_R0074
BBa_K195623	.	non_covalent_binding_site	698	720	.	+	0	ID=annotation319788;Name=dimer left half;Parent=BBa_R0074
BBa_K195623	.	promoter	713	718	.	+	0	ID=annotation319212;Name=-10;Parent=BBa_R0074
BBa_K195623	.	non_covalent_binding_site	722	743	.	+	0	ID=annotation319789;Name=dimer right half;Parent=BBa_R0074
BBa_K195623	.	terminator	2012	2140	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K195623	.	terminator	2100	2140	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K195623	.	polyA_site	2127	2140	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K195623	.	stem_loop	2107	2126	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K195623	.	stop_codon	2133	2133	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K195623	.	engineered_region	2100	2140	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K195623	.	terminator	2012	2091	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K195623	.	stem_loop	2023	2066	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K195623	.	engineered_region	2012	2091	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K195623	.	CDS	771	1064	.	+	0	ID=BBa_K116602;Name=BBa_K116602
BBa_K195623	.	ribosome_entry_site	57	68	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K195623	.	conserved_region	61	64	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K195623	.	ribosome_entry_site	1266	1277	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K195623	.	conserved_region	1270	1273	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K195623
agagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctttactagagaaagaggagaaatactagatgaa
aaaaataccacagatttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatactaatgag
gtaatcaaagagctcagtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatcaaaaaag
gcgctctcaaccaccataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatatagaggttaa
gtcacactcatttctcaaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgatcaactg
tcgggagaagaaatcaatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacgacgaaa
actacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagtcatttccaaccgaaaaaaggcttgcatttaaatcttacatatg
taatactttcaaagactacatttgtaagatttgtactagagaaagaggagaaatactagatggttcgtgcaaacaaacg
caacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtg
ggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaat
ggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggc
aaccgagcgttctgaacaaatccagatggagttctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagagagagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctttactaga
gaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggt
gatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattta
tttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgag
atacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatattt
ttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaa
aaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacat
catggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaacta
gcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaat
ctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacaca
tggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttata