##gff-version 3
##sequence-region BBa_K195624 1 1415
BBa_K195624	.	CDS	985	1278	.	+	0	ID=BBa_K116602;Name=BBa_K116602
BBa_K195624	.	promoter	1	48	.	+	0	ID=BBa_K116603;Name=BBa_K116603
BBa_K195624	.	CDS	75	759	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K195624	.	tag	695	728	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K195624	.	CDS	78	694	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K195624	.	engineered_tag	735	759	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K195624	.	ribosome_entry_site	57	68	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K195624	.	conserved_region	61	64	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K195624	.	promoter	905	958	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K195624	.	promoter	924	929	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K195624	.	engineered_region	905	958	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K195624	.	non_covalent_binding_site	905	923	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K195624	.	non_covalent_binding_site	930	948	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K195624	.	promoter	947	952	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K195624	.	terminator	768	896	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K195624	.	terminator	768	847	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K195624	.	stem_loop	779	822	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K195624	.	engineered_region	768	847	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K195624	.	terminator	856	896	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K195624	.	stem_loop	863	882	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K195624	.	polyA_site	883	896	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K195624	.	stop_codon	889	889	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K195624	.	engineered_region	856	896	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K195624	.	ribosome_entry_site	967	978	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K195624	.	conserved_region	971	974	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K195624	.	terminator	1287	1415	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K195624	.	terminator	1287	1366	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K195624	.	stem_loop	1298	1341	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K195624	.	engineered_region	1287	1366	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K195624	.	terminator	1375	1415	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K195624	.	stem_loop	1382	1401	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K195624	.	polyA_site	1402	1415	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K195624	.	stop_codon	1408	1408	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K195624	.	engineered_region	1375	1415	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K195624
agagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttcctttactagagaaagaggagaaatactagatgtc
cagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgt
aaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcct
tagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaa
taacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaa
aaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcac
tcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaac
acctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttc
ttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaa
actacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagaga
tactgagcactactagagaaagaggagaaatactagatggttcgtgcaaacaaacgcaacgaggctctacgaatcgaga
gtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcag
caggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatg
gctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatcc
agatggagttctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata