LOCUS       BBa_K195626              665 bp    DNA     linear       26-Jan-2026
DEFINITION  Toggle switch subunit (I) (pTet with repressor gene PenI)
ACCESSION   BBa_K195626
VERSION     BBa_K195626.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..467
                     /label=penI
     misc_feature    465..497
                     /label=LVA
     misc_feature    504..528
                     /label=Help:Barcodes
     CDS             81..528
                     /label=BBa_C0074
     stem_loop       548..591
                     /label=stem_loop
     misc_feature    537..616
                     /label=BBa_B0010
     terminator      537..616
                     /label=BBa_B0010
     stem_loop       632..651
                     /label=T7 TE
     misc_feature    658..658
                     /label=stop
     polyA_site      652..665
                     /label=polya
     misc_feature    625..665
                     /label=BBa_B0012
     terminator      625..665
                     /label=BBa_B0012
     terminator      537..665
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaaa taccacagat ttctgatgcg gaactcgaag
      121 tcatgaaagt gatttggaag cattcttcca ttaatactaa tgaggtaatc aaagagctca
      181 gtaaaacttc aacgtggagc ccaaaaacta ttcagactat gctgctgcgc cttatcaaaa
      241 aaggcgctct caaccaccat aaagaaggtc gggttttcgt ttacacgcct aatatagacg
      301 aatcagatta tatagaggtt aagtcacact catttctcaa ccggttttac aatggtacat
      361 taaattccat ggtactcaac tttttggaga atgatcaact gtcgggagaa gaaatcaatg
      421 aattgtatca gatactcgaa gaacataaga accgtaagaa ggaagctgca aacgacgaaa
      481 actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta ctagagccag
      541 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      601 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      661 ttata
//