LOCUS       BBa_K195627              925 bp    DNA     linear       03-May-2025
DEFINITION  Toggle switch subunit (II) (pPenI with repressor gene TetR)
ACCESSION   BBa_K195627
VERSION     BBa_K195627.1  
FEATURES             Location/Qualifiers
     misc_feature    1..77
                     /label=PenI
     promoter        23..28
                     /label=-35
     promoter        46..51
                     /label=-10
     misc_feature    31..53
                     /label=dimer left half
     misc_feature    55..76
                     /label=dimer right half
     promoter        1..77
                     /label=BBa_R0074
     misc_feature    90..93
                     /label=conserved
     RBS             86..97
                     /label=BBa_B0034
     CDS             107..723
                     /label=tetR
     misc_feature    724..757
                     /label=SsrA
     misc_feature    764..788
                     /label=Help:Barcodes
     CDS             104..788
                     /label=BBa_C0040
     stem_loop       808..851
                     /label=stem_loop
     misc_feature    797..876
                     /label=BBa_B0010
     terminator      797..876
                     /label=BBa_B0010
     stem_loop       892..911
                     /label=T7 TE
     misc_feature    918..918
                     /label=stop
     polyA_site      912..925
                     /label=polya
     misc_feature    885..925
                     /label=BBa_B0012
     terminator      885..925
                     /label=BBa_B0012
     terminator      797..925
                     /label=BBa_B0015
ORIGIN
        1 tcatttccaa ccgaaaaaag gcttgcattt aaatcttaca tatgtaatac tttcaaagac
       61 tacatttgta agatttgtac tagagaaaga ggagaaatac tagatgtcca gattagataa
      121 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
      181 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa
      241 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca
      301 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt
      361 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac
      421 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc
      481 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt
      541 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat
      601 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt
      661 cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg
      721 gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg
      781 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      841 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      901 tcgggtgggc ctttctgcgt ttata
//