##gff-version 3
##sequence-region BBa_K195628 1 1207
BBa_K195628	.	ribosome_entry_site	759	770	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K195628	.	conserved_region	763	766	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K195628	.	promoter	674	750	.	+	0	ID=BBa_R0074;Name=BBa_R0074
BBa_K195628	.	promoter	719	724	.	+	0	ID=annotation319212;Name=-10;Parent=BBa_R0074
BBa_K195628	.	non_covalent_binding_site	728	749	.	+	0	ID=annotation319789;Name=dimer right half;Parent=BBa_R0074
BBa_K195628	.	operator	674	750	.	+	0	ID=annotation302713;Name=PenI;Parent=BBa_R0074
BBa_K195628	.	non_covalent_binding_site	704	726	.	+	0	ID=annotation319788;Name=dimer left half;Parent=BBa_R0074
BBa_K195628	.	promoter	696	701	.	+	0	ID=annotation319211;Name=-35;Parent=BBa_R0074
BBa_K195628	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K195628	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K195628	.	terminator	1079	1207	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K195628	.	terminator	1167	1207	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K195628	.	stem_loop	1174	1193	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K195628	.	stop_codon	1200	1200	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K195628	.	polyA_site	1194	1207	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K195628	.	engineered_region	1167	1207	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K195628	.	terminator	1079	1158	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K195628	.	stem_loop	1090	1133	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K195628	.	engineered_region	1079	1158	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K195628	.	CDS	81	528	.	+	0	ID=BBa_C0074;Name=BBa_C0074
BBa_K195628	.	tag	465	497	.	+	0	ID=annotation306563;Name=LVA;Parent=BBa_C0074
BBa_K195628	.	engineered_tag	504	528	.	+	0	ID=annotation2214009;Name=Help:Barcodes;Parent=BBa_C0074
BBa_K195628	.	CDS	81	467	.	+	0	ID=annotation302638;Name=penI;Parent=BBa_C0074
BBa_K195628	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K195628	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K195628	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K195628	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K195628	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K195628	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K195628	.	CDS	777	1070	.	+	0	ID=BBa_K116602;Name=BBa_K116602
BBa_K195628	.	terminator	537	665	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K195628	.	terminator	625	665	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K195628	.	stem_loop	632	651	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K195628	.	stop_codon	658	658	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K195628	.	polyA_site	652	665	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K195628	.	engineered_region	625	665	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K195628	.	terminator	537	616	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K195628	.	stem_loop	548	591	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K195628	.	engineered_region	537	616	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K195628
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaaaataccacagatttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatact
aatgaggtaatcaaagagctcagtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatca
aaaaaggcgctctcaaccaccataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatataga
ggttaagtcacactcatttctcaaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgat
caactgtcgggagaagaaatcaatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacg
acgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagtcatttccaaccgaaaaaaggcttgcatttaaatctta
catatgtaatactttcaaagactacatttgtaagatttgtactagagaaagaggagaaatactagatggttcgtgcaaa
caaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaa
gctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttc
ttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgccc
ggcggcaaccgagcgttctgaacaaatccagatggagttctgatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata