BBa_K195628
type: ComponentDefinition
persistent identity: BBa K195628
display id: BBa_K195628
version: 1
was generated by: iGEM to SBOL conversion
description: Toggle switch (I)
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:17Z
mutable provenance: biobrick
owned by: james
owned by: myers
mutable description: This part is a toggle switch. The coding sequence PenI could repress BBa_R0074,the regulatory promoter pPenI.The coding sequence TetR could repress BBa_R0040,the regulatory promoter pTet.
discontinued: false
dominant: false
experience: experience/None
group u list: _292_
m user id: 0
owner id: 4841
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Yun-Hsiang Chang
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_B0015
component: BBa_R0040
component: BBa_C0074
component: BBa_B0034
component: BBa_R0074
component: BBa_K116602
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0074
sequence annotation: BBa_B0015
sequence annotation: BBa_R0074
sequence annotation: BBa_K116602
sequence: https://synbiohub.org/public/igem/BBa_K195628_sequence/1

BBa_B0015
type: Component
persistent identity: component2288083
display id: component2288083
version: 1
top level: BBa_K195628
definition: BBa_B0015
access: public

BBa_R0040
type: Component
persistent identity: component2288050
display id: component2288050
version: 1
top level: BBa_K195628
definition: p(tetR)
access: public

BBa_C0074
type: Component
persistent identity: component2288060
display id: component2288060
version: 1
top level: BBa_K195628
definition: penI
access: public

BBa_B0034
type: Component
persistent identity: component2288056
display id: component2288056
version: 1
top level: BBa_K195628
definition: BBa_B0034
access: public

BBa_R0074
type: Component
persistent identity: component2288073
display id: component2288073
version: 1
top level: BBa_K195628
definition: PenI
access: public

BBa_B0015
type: Component
persistent identity: component2288067
display id: component2288067
version: 1
top level: BBa_K195628
definition: BBa_B0015
access: public

BBa_K116602
type: Component
persistent identity: component2288076
display id: component2288076
version: 1
top level: BBa_K195628
definition: CII
access: public

BBa_B0034
type: Component
persistent identity: component2288075
display id: component2288075
version: 1
top level: BBa_K195628
definition: BBa_B0034
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2288050
display id: annotation2288050
version: 1
top level: BBa_K195628
location: https://synbiohub.org/public/igem/BBa_K195628/annotation2288050/range2288050/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K195628/annotation2288050/range2288050/1
type: Range
persistent identity: range2288050
display id: range2288050
version: 1
top level: BBa_K195628
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2288056
display id: annotation2288056
version: 1
top level: BBa_K195628
location: https://synbiohub.org/public/igem/BBa_K195628/annotation2288056/range2288056/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K195628/annotation2288056/range2288056/1
type: Range
persistent identity: range2288056
display id: range2288056
version: 1
top level: BBa_K195628
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_C0074
type: SequenceAnnotation
persistent identity: annotation2288060
display id: annotation2288060
version: 1
top level: BBa_K195628
location: https://synbiohub.org/public/igem/BBa_K195628/annotation2288060/range2288060/1
component: BBa_C0074

https://synbiohub.org/public/igem/BBa_K195628/annotation2288060/range2288060/1
type: Range
persistent identity: range2288060
display id: range2288060
version: 1
top level: BBa_K195628
direction: direction/reverse
start: 81
end: 528
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2288067
display id: annotation2288067
version: 1
top level: BBa_K195628
location: https://synbiohub.org/public/igem/BBa_K195628/annotation2288067/range2288067/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K195628/annotation2288067/range2288067/1
type: Range
persistent identity: range2288067
display id: range2288067
version: 1
top level: BBa_K195628
direction: direction/reverse
start: 537
end: 665
orientation: inline

BBa_R0074
type: SequenceAnnotation
persistent identity: annotation2288073
display id: annotation2288073
version: 1
top level: BBa_K195628
location: https://synbiohub.org/public/igem/BBa_K195628/annotation2288073/range2288073/1
component: BBa_R0074

https://synbiohub.org/public/igem/BBa_K195628/annotation2288073/range2288073/1
type: Range
persistent identity: range2288073
display id: range2288073
version: 1
top level: BBa_K195628
direction: direction/reverse
start: 674
end: 750
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2288075
display id: annotation2288075
version: 1
top level: BBa_K195628
location: https://synbiohub.org/public/igem/BBa_K195628/annotation2288075/range2288075/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K195628/annotation2288075/range2288075/1
type: Range
persistent identity: range2288075
display id: range2288075
version: 1
top level: BBa_K195628
direction: direction/reverse
start: 759
end: 770
orientation: inline

BBa_K116602
type: SequenceAnnotation
persistent identity: annotation2288076
display id: annotation2288076
version: 1
top level: BBa_K195628
location: https://synbiohub.org/public/igem/BBa_K195628/annotation2288076/range2288076/1
component: BBa_K116602

https://synbiohub.org/public/igem/BBa_K195628/annotation2288076/range2288076/1
type: Range
persistent identity: range2288076
display id: range2288076
version: 1
top level: BBa_K195628
direction: direction/reverse
start: 777
end: 1070
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2288083
display id: annotation2288083
version: 1
top level: BBa_K195628
location: https://synbiohub.org/public/igem/BBa_K195628/annotation2288083/range2288083/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K195628/annotation2288083/range2288083/1
type: Range
persistent identity: range2288083
display id: range2288083
version: 1
top level: BBa_K195628
direction: direction/reverse
start: 1079
end: 1207
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

CII
type: ComponentDefinition
persistent identity: BBa K116602
display id: BBa_K116602
version: 1
was generated by: iGEM to SBOL conversion
description: CII coding region from λ phage
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: λ phage
owned by: james
owned by: myers
mutable description: The CII coding region from λ phage.
discontinued: false
dominant: true
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none.
star: false
creator: Jesse Wu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K116602_sequence/1

PenI
type: ComponentDefinition
persistent identity: BBa R0074
display id: BBa_R0074
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (PenI regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: extends slightly past -50
star: true
creator: crackdots
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: PenI
sequence annotation: -35
sequence annotation: dimer left half
sequence annotation: -10
sequence annotation: dimer right half
sequence: https://synbiohub.org/public/igem/BBa_R0074_sequence/1

PenI
type: SequenceAnnotation
persistent identity: annotation302713
display id: annotation302713
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
type: Range
persistent identity: range302713
display id: range302713
version: 1
top level: PenI
direction: direction/forward
start: 1
end: 77
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319211
display id: annotation319211
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
type: Range
persistent identity: range319211
display id: range319211
version: 1
top level: PenI
direction: direction/forward
start: 23
end: 28
orientation: inline

dimer left half
type: SequenceAnnotation
persistent identity: annotation319788
display id: annotation319788
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
type: Range
persistent identity: range319788
display id: range319788
version: 1
top level: PenI
direction: direction/forward
start: 31
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation319212
display id: annotation319212
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
type: Range
persistent identity: range319212
display id: range319212
version: 1
top level: PenI
direction: direction/forward
start: 46
end: 51
orientation: inline

dimer right half
type: SequenceAnnotation
persistent identity: annotation319789
display id: annotation319789
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
type: Range
persistent identity: range319789
display id: range319789
version: 1
top level: PenI
direction: direction/forward
start: 55
end: 76
orientation: inline

penI
type: ComponentDefinition
persistent identity: BBa C0074
display id: BBa_C0074
version: 1
was generated by: iGEM to SBOL conversion
description: penI repressor from Bacillus licheniformis (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Since penI is being ported from Bacillus Licheniformis, the sequence was changed within the coding region to reflect codon usage in E.Coli K12. Codons were mapped to the codon of the same usage rank in E.Coli.
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: penI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0074_sequence/1

penI
type: SequenceAnnotation
persistent identity: annotation302638
display id: annotation302638
version: 1
top level: penI
location: https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1
type: Range
persistent identity: range302638
display id: range302638
version: 1
top level: penI
direction: direction/forward
start: 1
end: 387
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306563
display id: annotation306563
version: 1
top level: penI
location: https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1
type: Range
persistent identity: range306563
display id: range306563
version: 1
top level: penI
direction: direction/forward
start: 385
end: 417
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214009
display id: annotation2214009
version: 1
top level: penI
location: https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1
type: Range
persistent identity: range2214009
display id: range2214009
version: 1
top level: penI
direction: direction/forward
start: 424
end: 448
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K195628_sequence/1
type: Sequence
persistent identity: BBa K195628 sequence
display id: BBa_K195628_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaaaataccacagatttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatactaatgaggtaatcaaagagctcagtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatcaaaaaaggcgctctcaaccaccataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatatagaggttaagtcacactcatttctcaaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgatcaactgtcgggagaagaaatcaatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttgtactagagaaagaggagaaatactagatggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116602_sequence/1
type: Sequence
persistent identity: BBa K116602 sequence
display id: BBa_K116602_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0074_sequence/1
type: Sequence
persistent identity: BBa R0074 sequence
display id: BBa_R0074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0074_sequence/1
type: Sequence
persistent identity: BBa C0074 sequence
display id: BBa_C0074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaaaataccacagatttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatactaatgaggtaatcaaagagctcagtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatcaaaaaaggcgctctcaaccaccataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatatagaggttaagtcacactcatttctcaaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgatcaactgtcgggagaagaaatcaatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z