LOCUS       BBa_K195631             1512 bp    DNA     linear       22-Dec-2024
DEFINITION  pTet with inducer gene CII  and pRE with GFP generator
ACCESSION   BBa_K195631
VERSION     BBa_K195631.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..374
                     /label=BBa_K116602
     stem_loop       394..437
                     /label=stem_loop
     misc_feature    383..462
                     /label=BBa_B0010
     terminator      383..462
                     /label=BBa_B0010
     stem_loop       478..497
                     /label=T7 TE
     misc_feature    504..504
                     /label=stop
     polyA_site      498..511
                     /label=polya
     misc_feature    471..511
                     /label=BBa_B0012
     terminator      471..511
                     /label=BBa_B0012
     promoter        520..567
                     /label=BBa_K116603
     misc_feature    576..594
                     /label=TetR 1
     misc_feature    576..629
                     /label=BBa_R0040
     promoter        595..600
                     /label=-35
     misc_feature    601..619
                     /label=TetR 2
     promoter        618..623
                     /label=-10
     promoter        576..629
                     /label=BBa_R0040
     misc_feature    642..645
                     /label=conserved
     RBS             638..649
                     /label=BBa_B0034
     CDS             656..1375
                     /label=GFP protein
     CDS             656..1375
                     /label=BBa_E0040
     stem_loop       1395..1438
                     /label=stem_loop
     misc_feature    1384..1463
                     /label=BBa_B0010
     terminator      1384..1463
                     /label=BBa_B0010
     stem_loop       1479..1498
                     /label=T7 TE
     misc_feature    1505..1505
                     /label=stop
     polyA_site      1499..1512
                     /label=polya
     misc_feature    1472..1512
                     /label=BBa_B0012
     terminator      1472..1512
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggttcgtg caaacaaacg caacgaggct ctacgaatcg
      121 agagtgcgtt gcttaacaaa atcgcaatgc ttggaactga gaagacagcg gaagctgtgg
      181 gcgttgataa gtcgcagatc agcaggtgga agagggactg gattccaaag ttctcaatgc
      241 tgcttgctgt tcttgaatgg ggggtcgttg acgacgacat ggctcgattg gcgcgacaag
      301 ttgctgcgat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      361 agatggagtt ctgatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      421 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      481 tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagcctcgtt gcgtttgttt
      541 gcacgaacca tatgtaagta tttcctttac tagagtccct atcagtgata gagattgaca
      601 tccctatcag tgatagagat actgagcact actagagaaa gaggagaaat actagatgcg
      661 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      721 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
      781 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
      841 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
      901 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
      961 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1021 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     1081 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     1141 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     1201 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     1261 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     1321 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
     1381 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1441 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1501 tctgcgttta ta
//