LOCUS       BBa_K195632             3067 bp    DNA     linear       25-Nov-2024
DEFINITION  pPenI with GFP generator
ACCESSION   BBa_K195632
VERSION     BBa_K195632.1  
FEATURES             Location/Qualifiers
     misc_feature    1..77
                     /label=PenI
     promoter        23..28
                     /label=-35
     promoter        46..51
                     /label=-10
     misc_feature    31..53
                     /label=dimer left half
     misc_feature    55..76
                     /label=dimer right half
     promoter        1..77
                     /label=BBa_R0074
     misc_feature    90..93
                     /label=conserved
     RBS             86..97
                     /label=BBa_B0034
     CDS             104..826
                     /label=BBa_E0030
     stem_loop       846..889
                     /label=stem_loop
     misc_feature    835..914
                     /label=BBa_B0010
     terminator      835..914
                     /label=BBa_B0010
     stem_loop       930..949
                     /label=T7 TE
     misc_feature    956..956
                     /label=stop
     polyA_site      950..963
                     /label=polya
     misc_feature    923..963
                     /label=BBa_B0012
     terminator      923..963
                     /label=BBa_B0012
     promoter        2051..2181
                     /label=Pbad
     promoter        2014..2085
                     /label=PC
     misc_feature    972..2050
                     /label=AraC
     promoter        972..2181
                     /label=BBa_I0500
     misc_feature    2194..2197
                     /label=conserved
     RBS             2190..2201
                     /label=BBa_B0034
     CDS             2208..2930
                     /label=BBa_E0020
     stem_loop       2950..2993
                     /label=stem_loop
     misc_feature    2939..3018
                     /label=BBa_B0010
     terminator      2939..3018
                     /label=BBa_B0010
     stem_loop       3034..3053
                     /label=T7 TE
     misc_feature    3060..3060
                     /label=stop
     polyA_site      3054..3067
                     /label=polya
     misc_feature    3027..3067
                     /label=BBa_B0012
     terminator      3027..3067
                     /label=BBa_B0012
ORIGIN
        1 tcatttccaa ccgaaaaaag gcttgcattt aaatcttaca tatgtaatac tttcaaagac
       61 tacatttgta agatttgtac tagagaaaga ggagaaatac tagatggtga gcaagggcga
      121 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      181 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      241 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
      301 ctacggcctg caatgcttcg cccgctaccc cgaccacatg aagctgcacg acttcttcaa
      361 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      421 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      481 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      541 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      601 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      661 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
      721 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      781 cgccgccggg atcactctcg gcatggacga gctgtacaag taataatact agagccaggc
      841 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      901 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      961 atatactaga gttatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
     1021 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
     1081 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
     1141 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
     1201 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
     1261 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga
     1321 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
     1381 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
     1441 atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc
     1501 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
     1561 taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct
     1621 cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc
     1681 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
     1741 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
     1801 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
     1861 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
     1921 gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg
     1981 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
     2041 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
     2101 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
     2161 tacccgtttt tttgggctag ctactagaga aagaggagaa atactagatg gtgagcaagg
     2221 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg
     2281 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2341 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2401 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2461 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2521 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2581 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2641 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     2701 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2761 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2821 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2881 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc
     2941 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     3001 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     3061 gtttata
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