BBa_K1957001
1
BBa_K1957001
HydC subunit of FeFe Hydrogenase
2016-10-05T11:00:00Z
2016-10-06T10:13:06Z
The source of the part will be its sequence which was retrieved from GenBank.
One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically this is the HydC subunit, which is the outer periplasmic subunit. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.
false
false
_2424_
32225
32225
9
false
This is one of of the three FeFe Hydrogenase subunits. To make the entire FeFe Hydrogenase construct the following BioBricks need to be ligated with BBa_K1957001: BBa_K1957004 with BBa_K1957002 or BBa_K1957004 with BBa_K1957003.
The entire construct couldn't conjugate properly into S.oneidensis, thus the three subunits were ligated using golden gate cloning into the pBAD expression vector.
false
Nancy Teng
BBa_K1957001_sequence
1
ggtctccgaaatgcatcaagaacatgatatgcgtcccattttaaaacatccgctaaacgtcaggattttccactacattctgctgctcagctttttgccattagccgcaacaggtttgctattgttttttaaaccgctttcccaagaaggtatgcagctaacctatgatgtgcacatcattgcggggattgttatggccctcgatgccgtggcatttaccatcatggcatttgaccgagtggtactgttcatcgcccgcgtgtttagcttctcagggcgtgatgtgaaatggtttatggtgttaggcggctacccacagaaattcttactgggtaaaaaagtacctattccaccgatgaacaaatacaactcaggccaaaaactctttggtgcctgcgtactgattggcggcacagtgcttatcctctccggtttagtgctgtggttcattccccacgctgcgccgcgggatatagtgtggtttttaggtcaggcccatttagtgtcaggcttagtgttaaccgccttcttgcccgtgcatttattccttgctgtgtaccgctttgatgatttcaaggccatgatgatccacggtaatgtgccttaccatgatgccgctgagtacacccctctgtgggttaaaaatgaaattgcccccgtcgccactaacggtgaacaactgacaagcaaatagtggaggagacc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z