BBa_K1957005 1 BBa_K1957005 HyaA subunit of NiFe Hydrogenase 2016-10-05T11:00:00Z 2016-10-06T10:21:13Z The source of the part will be its sequence which was retrieved from GenBank. One of the three subunits that make up NiFe Hydrogenase in Shewanella oneidensis. Specifically this is the HyaA subunit which is the membrane bound catalytic subunit. This unit is one out of the three subunits that can be used to make a the entire NiFe hydrogenase construct. false false _2424_ 32225 32225 9 false This is one of of the three NiFe Hydrogenase subunits. The entire NiFe construct can be made by ligating the remaining units into the pBAD expression vector via golden gate cloning. pBAD was used as opposed to pSB1C3 as pSB1C3 would not conjugate into S.oneidensis. false Nancy Teng BBa_K1957005_sequence 1 ggtctccacccatggacacacatgcagccctctatgaacagggaaaggcgcgcttagatgcactgcgccaattcgctccccgtcaacagcaaacattactcgaaaaattacagcaacacggcatctcacgccgtgattttatgaaatggagtgccatggtgacggggatgctcgccctaccactcccctttagtaacttagttgctgaagcggccgaactcgccgaccgtgtacccttaatttggttacatatggctgaatgtactggctgctccgaatccttagttcgtgccgatacccccaacttagattcgctgatctttgatcatatctctttggaatatcacgaaaccttaatggctgctgccggttggcaagctgaggaaaacctagagcacgcactcgaaacctacaaggggcgctatttactggccgttgaaggagcaatcccaaccgccaataacggcagtttcttaaccgtaggttgtaaaggccacacaggtctggaaatcgtcaaacatgcagctgaaggcgccgctgcgattatctctgttggcacttgtgcagcatttggtggcgtgcaagccgcgtaccctaaccctacgggggctaagggcgtgcacgaagtggtcagcaaacccgtgattaacttaggtggttgcccaccgagcgaaaagaacatcgtcggcaccctgatgtatttcatcatgttcggtaaattgcccgcactcgatatgttcaaccgtccaaaatgggcctatggcgcacgggtacatgataactgtgaacgtcgtggccgttttgatgcaggtgagttcgttgaagaatttggcgatcacggcgcgaaagaaggctactgcctctataaagtcggttgtaaaggtccctacacctataataactgcccaacggaacgcttcaaccaccacaccagctggccagtgttagcgggccacggctgtatgggctgctctgaacctaacttctgggacgatatggctgactttgaaaaaccccttggccgtcaactgctccatggattggatgcaaccgcagacaccgtcggtgcggtgattttaggcgcgacagtcgttggcattggcgcccacgccgttgccagtatttttgccaagccgctggaggaataacggagacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z