BBa_K1957009
1
BBa_K1957009
FeFe Hydrogenase
2016-10-12T11:00:00Z
2016-10-13T12:05:13Z
The source of the part will be its sequence which was retrieved from GenBank.
Entire FeFe Hydrogenase cluster ligated together and inserted into pSB1C3. This cluster has a Strep-II tag on the N-terminus, thus is made out of the following BioBricks: Bba_K1957001, Bba_K1957003 and Bba_K1957004.
false
false
_2424_
32225
32225
9
false
The three BioBricks were originally ligated together in pBAD. This was then extracted out of the pBAD vector and re-inserted into pSB1C3. We suggest that other iGEM teams wanting to use this construct excise the FeFe cluster out of pSB1C3 and ligate it into pBAD.
false
Nancy Teng
BBa_K1957009_sequence
1
atgacaacgacaacttatcaaccaggagaaatccaagggctgatcaagattaatgcatccaaatgcaaaggatgtgatgcctgtaaacaattctgcccaacccatgccattaatggcgcttcgggtgcagtacactctatcgatgaagataaatgcttaagctgcggacagtgtttaattaactgtccatttagcgctattgaggaaacccacagcgcacttgaaaccgtgattaaaaagctcgctgataaaaataccaccgtggtcgggattatcgcgcctgcggtacgggtggcgattggtgaagaatttggcttaggtacaggtgagctagtaacaggcaaactctacggtgccatgaatcaagctggctttaaaattttcgactgtaacttcgccgccgatttgaccattatggaagaaggcagtgagtttattcatcgcctgcacgccaatgtaaaaggtgaagctaacgcaggcccattgccgcaatttacctcctgctgcccaggctgggtacgctacctcgaaacccgctaccctgcacttttacctaacctatcgaccgccaaatcacctcagcaaatggcagggactgtcgccaaaacctacggcgccaaggtatatcaaatgcagccagagaatattttcactgtctctgtaatgccttgcacctcgaaaaagctcgaagcctcccgtcccgaatttaactcggcttggcaatatcatcaggaacacggcgcaaactcgccctcctaccaagatattgatgccgtgctcaccacaagggaaatggctcagttactcaaactgctcgatatcgatctcgcgaataccgcggaatatcaaggcgatagtttgttctctgaatacactggcgcgggcacaatttttggaacaaccggcggggtgatggaagcggcgctgcgtaccgcccataaagtactgactggaactgaaatggctaagctggaatttgaacccgtacgcgggctaaaaggcgtgaaatcagcctctgtcagcctgtttgatacagagcttaaccaagatgtgaccgtcaatgtcgccgtagtgcacgacatgggcaacaacattgagcccgtactgcgcgatgtgatggctggcacctctccttatcactttattgaggtgatgaactgcgctggcggttgcgtcaacggcggaggccaacctattgaaggtaaaggctcttcatggctgggtaacatttaacgggaaacagaaatgtggagccatccacaattcgagaagaacaagaaaaaacacctatttgccgaggacagtttctttctgtcacgccgtaaatttatggctgtcggtgccgcgtttgtggccgcactcgcgatccccatcggctggtttaccagcaagcttgaacgccgtaatgagtacattaaggccagaagccaagggctatacaaggacgacagcctagcaaaaacccgcgtcagccatgctaaccccgcggtagaaaagtactacaaagagttcggtggcgagccattaggacatatgtcccacgagctactgcacacccactttgtcgatcgcaccaaattaagctcttgattggagatgagaaatgcatcaagaacatgatatgcgtcccattttaaaacatccgctaaacgtcaggattttccactacattctgctgctcagctttttgccattagccgcaacaggtttgctattgttttttaaaccgctttcccaagaaggtatgcagctaacctatgatgtgcacatcattgcggggattgttatggccctcgatgccgtggcatttaccatcatggcatttgaccgagtggtactgttcatcgcccgcgtgtttagcttctcagggcgtgatgtgaaatggtttatggtgttaggcggctacccacagaaattcttactgggtaaaaaagtacctattccaccgatgaacaaatacaactcaggccaaaaactctttggtgcctgcgtactgattggcggcacagtgcttatcctctccggtttagtgctgtggttcattccccacgctgcgccgcgggatatagtgtggtttttaggtcaggcccatttagtgtcaggcttagtgttaaccgccttcttgcccgtgcatttattccttgctgtgtaccgctttgatgatttcaaggccatgatgatccacggtaatgtgccttaccatgatgccgctgagtacacccctctgtgggttaaaaatgaaattgcccccgtcgccactaacggtgaacaactgacaagcaaatagtgga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z