BBa_K1957009 1 BBa_K1957009 FeFe Hydrogenase 2016-10-12T11:00:00Z 2016-10-13T12:05:13Z The source of the part will be its sequence which was retrieved from GenBank. Entire FeFe Hydrogenase cluster ligated together and inserted into pSB1C3. This cluster has a Strep-II tag on the N-terminus, thus is made out of the following BioBricks: Bba_K1957001, Bba_K1957003 and Bba_K1957004. false false _2424_ 32225 32225 9 false The three BioBricks were originally ligated together in pBAD. This was then extracted out of the pBAD vector and re-inserted into pSB1C3. We suggest that other iGEM teams wanting to use this construct excise the FeFe cluster out of pSB1C3 and ligate it into pBAD. false Nancy Teng BBa_K1957009_sequence 1 atgacaacgacaacttatcaaccaggagaaatccaagggctgatcaagattaatgcatccaaatgcaaaggatgtgatgcctgtaaacaattctgcccaacccatgccattaatggcgcttcgggtgcagtacactctatcgatgaagataaatgcttaagctgcggacagtgtttaattaactgtccatttagcgctattgaggaaacccacagcgcacttgaaaccgtgattaaaaagctcgctgataaaaataccaccgtggtcgggattatcgcgcctgcggtacgggtggcgattggtgaagaatttggcttaggtacaggtgagctagtaacaggcaaactctacggtgccatgaatcaagctggctttaaaattttcgactgtaacttcgccgccgatttgaccattatggaagaaggcagtgagtttattcatcgcctgcacgccaatgtaaaaggtgaagctaacgcaggcccattgccgcaatttacctcctgctgcccaggctgggtacgctacctcgaaacccgctaccctgcacttttacctaacctatcgaccgccaaatcacctcagcaaatggcagggactgtcgccaaaacctacggcgccaaggtatatcaaatgcagccagagaatattttcactgtctctgtaatgccttgcacctcgaaaaagctcgaagcctcccgtcccgaatttaactcggcttggcaatatcatcaggaacacggcgcaaactcgccctcctaccaagatattgatgccgtgctcaccacaagggaaatggctcagttactcaaactgctcgatatcgatctcgcgaataccgcggaatatcaaggcgatagtttgttctctgaatacactggcgcgggcacaatttttggaacaaccggcggggtgatggaagcggcgctgcgtaccgcccataaagtactgactggaactgaaatggctaagctggaatttgaacccgtacgcgggctaaaaggcgtgaaatcagcctctgtcagcctgtttgatacagagcttaaccaagatgtgaccgtcaatgtcgccgtagtgcacgacatgggcaacaacattgagcccgtactgcgcgatgtgatggctggcacctctccttatcactttattgaggtgatgaactgcgctggcggttgcgtcaacggcggaggccaacctattgaaggtaaaggctcttcatggctgggtaacatttaacgggaaacagaaatgtggagccatccacaattcgagaagaacaagaaaaaacacctatttgccgaggacagtttctttctgtcacgccgtaaatttatggctgtcggtgccgcgtttgtggccgcactcgcgatccccatcggctggtttaccagcaagcttgaacgccgtaatgagtacattaaggccagaagccaagggctatacaaggacgacagcctagcaaaaacccgcgtcagccatgctaaccccgcggtagaaaagtactacaaagagttcggtggcgagccattaggacatatgtcccacgagctactgcacacccactttgtcgatcgcaccaaattaagctcttgattggagatgagaaatgcatcaagaacatgatatgcgtcccattttaaaacatccgctaaacgtcaggattttccactacattctgctgctcagctttttgccattagccgcaacaggtttgctattgttttttaaaccgctttcccaagaaggtatgcagctaacctatgatgtgcacatcattgcggggattgttatggccctcgatgccgtggcatttaccatcatggcatttgaccgagtggtactgttcatcgcccgcgtgtttagcttctcagggcgtgatgtgaaatggtttatggtgttaggcggctacccacagaaattcttactgggtaaaaaagtacctattccaccgatgaacaaatacaactcaggccaaaaactctttggtgcctgcgtactgattggcggcacagtgcttatcctctccggtttagtgctgtggttcattccccacgctgcgccgcgggatatagtgtggtttttaggtcaggcccatttagtgtcaggcttagtgttaaccgccttcttgcccgtgcatttattccttgctgtgtaccgctttgatgatttcaaggccatgatgatccacggtaatgtgccttaccatgatgccgctgagtacacccctctgtgggttaaaaatgaaattgcccccgtcgccactaacggtgaacaactgacaagcaaatagtgga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z