BBa_K1958007
1
PbrR -> C.
PbrR -> C. metallidurans
2016-10-11T11:00:00Z
2016-10-12T09:35:51Z
Cupriavidus metallidurans CH34
PbrR is amplified from Cupriavidus metallidurans CH34 (formerly Ralstonia metallidurans) genome.
PbrR, together with its homologues in the same bacterium, are the only known lead(II)-specific binding protein found in nature. PbrR binds lead(II) 1000-fold more selectively over other metal ions such as mercury(II), zinc(II), copper(II), nickel(II), and silver(I). Besides lead(II), PbrR also shows the binding capacity of cadmium(II), but slightly lower than that of lead(II). The binding capacity may be concerned with three conserved Cys residues: Cys78, Cys113 and Cys122.
Because the gene encoding PbrR contains a digest site of Pst I (-CTGCAG-), to fit the construction of standard biobrick, we change those site from -CTGCAG- to -CTGCAA- by point mutation.
false
false
_2425_
21319
21319
9
true
None.
false
Wei Wei
annotation2493974
1
PbrR coding region
range2493974
1
1
438
annotation2493981
1
TAG stop codon
range2493981
1
436
438
annotation2493972
1
ATG start codon
range2493972
1
1
3
BBa_K1958007_sequence
1
atgaatatccagatcggcgagcttgccaagcgcaccgcatgcccggtggtgaccattcgcttctacgaacaagaagggctgttgccgccgccgggccgcagccgggggaattttcgcctgtatggcgaggagcacgtggagcgcttgcagttcattcgtcactgccggtctctggatatgccgttgagcgacgtacggaccttattgagttaccggaagcggcccgaccaggattgcggtgaagtcaatatgctcttagatgagcacatccgtcaggtcgaatctcggatcggagccttgctcgaactgaagcaccatttggtggaactgcgcgaagcctgttctggtgccaggcccgcccaatcgtgcgggattctgcaaggactgtcggactgcgtgtgtgatacgcgggggaccaccgcccatccaagcgactag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z