BBa_K1959003
1
BBa_K1959003
BKT coding sequence fused with <em>Pea</em> transit peptide
2016-10-03T11:00:00Z
2016-10-24T02:02:32Z
Original sequence can be accessed from Genebank, Genebank accession: AY860820.1.
This part is consist of a Pea transit peptide and the coding sequence of beta-carotene ketolase originally derived from
Chlamydomonas reinhardtii. Beta-carotene ketolase realizes the convertion of zeaxanthin to astaxanthin, the final step of astaxanthin biosynthesis. Sequence of beta-carotene ketolase has been optimized for better expression in Orazy sativa.
false
false
_2426_
29884
29884
9
false
A pea transit peptide has been functionally fused to the BKT cds. The BKT codon has made some optimization for expression in rice. An Pst I restriction site has been mutated by site-directed mutation.
false
Junyu Chen
annotation2486169
1
Initiation codon of BKT
range2486169
1
172
174
annotation2485802
1
Pst I mutant
range2485802
1
1036
1041
annotation2486170
1
Initiation codon of Pea transit peptide
range2486170
1
1
3
annotation2486168
1
Pea transit peptide
range2486168
1
1
171
annotation2486171
1
Termination codon of BKT
range2486171
1
1156
1158
annotation2485803
1
BKT
range2485803
1
172
1158
BBa_K1959003_sequence
1
atggcttctatgatatcctcttccgctgtgacaacagtcagccgtgcctctagggggcaatccgccgcagtggctccattcggcggcctcaaatccatgactggattcccagtgaagaaggtcaacactgacattacttccattacaagcaatggtggaagagtaaagtgcatgggtccaggcatccagcctacatctgcccggccgtgctctcgtaccaagcactcccgttttgccctcttggccgctgcgctgactgcaaggcgggtcaaacagtttactaagcagttccgctcacgccgtatggccgaggacattcttaaactttggcagagacaatatcatctgccacgggaggacagcgacaagagaaccctccgggaaagggtgcatctttacaggcctcctagatcggatctgggcggtatcgcagtggccgtcacggtgatcgcgttgtgggccacactctttgtgtatggtctctggtttgtcaagcttccttgggcattgaaggtgggcgagacggccacctcatgggctacgattgctgctgtgtttttctccttggagttcttgtacactggactcttcatcactacccatgacgcaatgcatggcactattgccctcaggaacaggaggctcaacgacttcctgggccagttggcgatttcgttgtacgcttggttcgactactcagtccttcaccggaagcattgggagcatcacaatcataccggggaacccagggttgatcccgattttcaccgcggcaatccaaatctcgcggtctggttcgcgcagttcatggtgagctatatgacgctctcgcaatttcttaaaatagcagtatggagtaatctgctccttttggctggcgctccactcgctaaccagctgctcttcatgactgccgcgccgatactctcagcttttcggttgttttactacggcacctacgtccctcaccacccagagaaaggacacacaggggcgatgccatggcaagtgagccgcacttcgtcagcgagtcggctacagagctttcttacgtgttatcacttcgatcttcattgggaacatcaccggtggccttacgccccgtggtgggaactcccaaagtgcagacagatagctcgcggggctgcgctcgcctag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z