BBa_K1959003 1 BBa_K1959003 BKT coding sequence fused with <em>Pea</em> transit peptide 2016-10-03T11:00:00Z 2016-10-24T02:02:32Z Original sequence can be accessed from Genebank, Genebank accession: AY860820.1. This part is consist of a Pea transit peptide and the coding sequence of beta-carotene ketolase originally derived from Chlamydomonas reinhardtii. Beta-carotene ketolase realizes the convertion of zeaxanthin to astaxanthin, the final step of astaxanthin biosynthesis. Sequence of beta-carotene ketolase has been optimized for better expression in Orazy sativa. false false _2426_ 29884 29884 9 false A pea transit peptide has been functionally fused to the BKT cds. The BKT codon has made some optimization for expression in rice. An Pst I restriction site has been mutated by site-directed mutation. false Junyu Chen annotation2486169 1 Initiation codon of BKT range2486169 1 172 174 annotation2485802 1 Pst I mutant range2485802 1 1036 1041 annotation2486170 1 Initiation codon of Pea transit peptide range2486170 1 1 3 annotation2486168 1 Pea transit peptide range2486168 1 1 171 annotation2486171 1 Termination codon of BKT range2486171 1 1156 1158 annotation2485803 1 BKT range2485803 1 172 1158 BBa_K1959003_sequence 1 atggcttctatgatatcctcttccgctgtgacaacagtcagccgtgcctctagggggcaatccgccgcagtggctccattcggcggcctcaaatccatgactggattcccagtgaagaaggtcaacactgacattacttccattacaagcaatggtggaagagtaaagtgcatgggtccaggcatccagcctacatctgcccggccgtgctctcgtaccaagcactcccgttttgccctcttggccgctgcgctgactgcaaggcgggtcaaacagtttactaagcagttccgctcacgccgtatggccgaggacattcttaaactttggcagagacaatatcatctgccacgggaggacagcgacaagagaaccctccgggaaagggtgcatctttacaggcctcctagatcggatctgggcggtatcgcagtggccgtcacggtgatcgcgttgtgggccacactctttgtgtatggtctctggtttgtcaagcttccttgggcattgaaggtgggcgagacggccacctcatgggctacgattgctgctgtgtttttctccttggagttcttgtacactggactcttcatcactacccatgacgcaatgcatggcactattgccctcaggaacaggaggctcaacgacttcctgggccagttggcgatttcgttgtacgcttggttcgactactcagtccttcaccggaagcattgggagcatcacaatcataccggggaacccagggttgatcccgattttcaccgcggcaatccaaatctcgcggtctggttcgcgcagttcatggtgagctatatgacgctctcgcaatttcttaaaatagcagtatggagtaatctgctccttttggctggcgctccactcgctaaccagctgctcttcatgactgccgcgccgatactctcagcttttcggttgttttactacggcacctacgtccctcaccacccagagaaaggacacacaggggcgatgccatggcaagtgagccgcacttcgtcagcgagtcggctacagagctttcttacgtgttatcacttcgatcttcattgggaacatcaccggtggccttacgccccgtggtgggaactcccaaagtgcagacagatagctcgcggggctgcgctcgcctag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z