LOCUS BBa_K1960000 5347 bp DNA linear 29-Nov-2024 DEFINITION Sensing device with switch for gram-negative bacterium ACCESSION BBa_K1960000 VERSION BBa_K1960000.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 62..63 /label=prefix misc_feature 235..235 /label=T misc_feature 553..553 /label=A CDS 62..811 /label=luxR misc_feature 62..817 /label=BBa_C0062 misc_feature 818..842 /label=Help:Barcodes CDS 62..817 /label=BBa_C0062 stem_loop 862..905 /label=stem_loop misc_feature 851..930 /label=BBa_B0010 terminator 851..930 /label=BBa_B0010 stem_loop 946..965 /label=T7 TE misc_feature 972..972 /label=stop polyA_site 966..979 /label=polya misc_feature 939..979 /label=BBa_B0012 terminator 939..979 /label=BBa_B0012 misc_feature 988..1007 /label=LuxR/HSL promoter 1007..1012 /label=-35 promoter 1029..1034 /label=-10 misc_feature 1040..1040 /label=start misc_feature 988..1042 /label=BBa_R0062 promoter 988..1042 /label=BBa_R0062 misc_feature 44..1042 /label=BBa_A340620 misc_feature 1055..1058 /label=conserved RBS 1051..1062 /label=BBa_B0034 CDS 1069..1791 /label=BBa_E0020 misc_feature 1051..1791 /label=BBa_S01022 misc_feature 1804..1807 /label=conserved RBS 1800..1811 /label=BBa_B0034 CDS 1818..2903 /label=BBa_I732100 stem_loop 2923..2966 /label=stem_loop misc_feature 2912..2991 /label=BBa_B0010 terminator 2912..2991 /label=BBa_B0010 stem_loop 3007..3026 /label=T7 TE misc_feature 3033..3033 /label=stop polyA_site 3027..3040 /label=polya misc_feature 3000..3040 /label=BBa_B0012 terminator 3000..3040 /label=BBa_B0012 misc_feature 1800..3040 /label=BBa_I732820 misc_feature 3051..3067 /label=lac O1 promoter 3068..3073 /label=-35 misc_feature 3074..3090 /label=lac O1 promoter 3091..3096 /label=-10 misc_feature 3049..3102 /label=BBa_R0011 promoter 3049..3102 /label=BBa_R0011 misc_feature 3116..3119 /label=conserved RBS 3112..3123 /label=BBa_B0034 CDS 3133..3840 /label=cI lambda misc_feature 3841..3873 /label=LVA misc_feature 3880..3904 /label=Help:Barcodes CDS 3130..3879 /label=BBa_C0051 stem_loop 3924..3967 /label=stem_loop misc_feature 3913..3992 /label=BBa_B0010 terminator 3913..3992 /label=BBa_B0010 stem_loop 4008..4027 /label=T7 TE misc_feature 4034..4034 /label=stop polyA_site 4028..4041 /label=polya misc_feature 4001..4041 /label=BBa_B0012 terminator 4001..4041 /label=BBa_B0012 misc_feature 3112..4041 /label=BBa_P0451 promoter 4087..4092 /label=-10 promoter 4064..4069 /label=-35 misc_feature 4074..4090 /label=OR1 misc_feature 4050..4066 /label=OR2 misc_feature 4050..4098 /label=BBa_R0051 promoter 4050..4098 /label=BBa_R0051 misc_feature 4111..4114 /label=conserved RBS 4107..4118 /label=BBa_B0034 CDS 4125..5210 /label=BBa_I732100 stem_loop 5230..5273 /label=stem_loop misc_feature 5219..5298 /label=BBa_B0010 terminator 5219..5298 /label=BBa_B0010 stem_loop 5314..5333 /label=T7 TE misc_feature 5340..5340 /label=stop polyA_site 5334..5347 /label=polya misc_feature 5307..5347 /label=BBa_B0012 terminator 5307..5347 /label=BBa_B0012 misc_feature 4107..5347 /label=BBa_I732820 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag 121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata 181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga 241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc 301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa 361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac 421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca 481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt 541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa 601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc 661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa 721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac 781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc 841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa 1021 tggtttgtta tagtcgaata aatactagag aaagaggaga aatactagat ggtgagcaag 1081 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac 1141 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 1201 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 1261 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 1321 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 1381 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 1441 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 1501 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc 1561 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 1621 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 1681 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 1741 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata atactagaga 1801 aagaggagaa atactagatg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg 1861 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc 1921 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac 1981 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg 2041 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg 2101 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc 2161 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg 2221 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac 2281 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg 2341 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc 2401 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg 2461 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg 2521 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc 2581 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg 2641 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc 2701 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg 2761 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc 2821 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 2881 gactggaaag cgggcagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag 2941 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 3001 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa ttgtgagcgg 3061 ataacaattg acattgtgag cggataacaa gatactgagc acatactaga gaaagaggag 3121 aaatactaga tgagcacaaa aaagaaacca ttaacacaag agcagcttga ggacgcacgt 3181 cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc 3241 gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg catcaatgca 3301 ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt tgaagaattt 3361 agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat gcagccgtca 3421 cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat gttctcacct 3481 gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac caaaaaagcc 3541 agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc 3601 aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca 3661 ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa actgatcagg 3721 gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat cccatgcaat 3781 gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga gacgtttggc 3841 gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg ctagtgtaga 3901 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 3961 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 4021 gtgggccttt ctgcgtttat atactagagt aacaccgtgc gtgttgacta ttttacctct 4081 ggcggtgata atggttgcta ctagagaaag aggagaaata ctagatgaaa ccagtaacgt 4141 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc 4201 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga 4261 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg 4321 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc 4381 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag 4441 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact 4501 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt 4561 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg 4621 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag 4681 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca 4741 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt 4801 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca 4861 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg 4921 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt 4981 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc 5041 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa 5101 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 5161 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtaataa tactagagcc 5221 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 5281 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 5341 gtttata //