##gff-version 3
##sequence-region BBa_K1960000 1 5347
BBa_K1960000	.	promoter	3049	3102	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1960000	.	operator	3051	3067	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1960000	.	promoter	3068	3073	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1960000	.	promoter	3091	3096	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1960000	.	operator	3074	3090	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1960000	.	engineered_region	3049	3102	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1960000	.	promoter	4050	4098	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K1960000	.	non_covalent_binding_site	4074	4090	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1960000	.	promoter	4064	4069	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1960000	.	promoter	4087	4092	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1960000	.	engineered_region	4050	4098	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1960000	.	non_covalent_binding_site	4050	4066	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1960000	.	engineered_region	44	1042	.	+	0	ID=BBa_A340620;Name=BBa_A340620
BBa_K1960000	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_A340620
BBa_K1960000	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1960000	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1960000	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1960000	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1960000	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_A340620
BBa_K1960000	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1960000	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_A340620
BBa_K1960000	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1960000	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1960000	.	promoter	988	1042	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_A340620
BBa_K1960000	.	start_codon	1040	1040	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1960000	.	promoter	1007	1012	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1960000	.	promoter	1029	1034	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1960000	.	engineered_region	988	1042	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1960000	.	non_covalent_binding_site	988	1007	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1960000	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_A340620
BBa_K1960000	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1960000	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1960000	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1960000	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1960000	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1960000	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1960000	.	engineered_region	1800	3040	.	+	0	ID=BBa_I732820;Name=BBa_I732820
BBa_K1960000	.	CDS	1818	2903	.	+	0	ID=BBa_I732100;Name=BBa_I732100;Parent=BBa_I732820
BBa_K1960000	.	ribosome_entry_site	1800	1811	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732820
BBa_K1960000	.	conserved_region	1804	1807	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1960000	.	terminator	2912	2991	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732820
BBa_K1960000	.	stem_loop	2923	2966	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1960000	.	engineered_region	2912	2991	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1960000	.	terminator	3000	3040	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732820
BBa_K1960000	.	polyA_site	3027	3040	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1960000	.	stem_loop	3007	3026	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1960000	.	engineered_region	3000	3040	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1960000	.	stop_codon	3033	3033	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1960000	.	engineered_region	3112	4041	.	+	0	ID=BBa_P0451;Name=BBa_P0451
BBa_K1960000	.	ribosome_entry_site	3112	3123	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0451
BBa_K1960000	.	conserved_region	3116	3119	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1960000	.	terminator	3913	3992	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0451
BBa_K1960000	.	stem_loop	3924	3967	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1960000	.	engineered_region	3913	3992	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1960000	.	CDS	3130	3879	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0451
BBa_K1960000	.	tag	3841	3873	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1960000	.	CDS	3133	3840	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1960000	.	engineered_tag	3880	3904	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1960000	.	terminator	4001	4041	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0451
BBa_K1960000	.	polyA_site	4028	4041	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1960000	.	stem_loop	4008	4027	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1960000	.	engineered_region	4001	4041	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1960000	.	stop_codon	4034	4034	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1960000	.	engineered_region	1051	1791	.	+	0	ID=BBa_S01022;Name=BBa_S01022
BBa_K1960000	.	CDS	1069	1791	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_S01022
BBa_K1960000	.	ribosome_entry_site	1051	1062	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S01022
BBa_K1960000	.	conserved_region	1055	1058	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1960000	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1960000	.	engineered_region	4107	5347	.	+	0	ID=BBa_I732820;Name=BBa_I732820
BBa_K1960000	.	CDS	4125	5210	.	+	0	ID=BBa_I732100;Name=BBa_I732100;Parent=BBa_I732820
BBa_K1960000	.	ribosome_entry_site	4107	4118	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732820
BBa_K1960000	.	conserved_region	4111	4114	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1960000	.	terminator	5219	5298	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732820
BBa_K1960000	.	stem_loop	5230	5273	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1960000	.	engineered_region	5219	5298	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1960000	.	terminator	5307	5347	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732820
BBa_K1960000	.	polyA_site	5334	5347	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1960000	.	stem_loop	5314	5333	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1960000	.	engineered_region	5307	5347	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1960000	.	stop_codon	5340	5340	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K1960000
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttg
ttatagtcgaataaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggt
gcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacc
tacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctga
cctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaagg
ctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgac
accctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtaca
actacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaa
catcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgccc
gacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagt
tcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagaaagaggagaaatactag
atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccagg
ccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggc
acaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaatt
gtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaag
cctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccagga
tgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagt
attattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgt
tagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattca
gccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatc
gttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcg
ttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaa
acaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaat
cagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttgg
ccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagc
ggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaag
agcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgt
cgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgcc
gcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatg
aagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctc
acctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattc
tggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcg
ttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaact
gatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttcc
gttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtag
cttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaa
agaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcc
cgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattaca
ttcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgca
cgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaa
cgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatc
cgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgacca
gacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcac
cagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctca
ctcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaat
gctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattacc
gagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgc
cgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggcca
ggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcc
tctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata