##gff-version 3
##sequence-region BBa_K1960002 1 3548
BBa_K1960002	.	promoter	1250	1303	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1960002	.	promoter	1292	1297	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1960002	.	engineered_region	1250	1303	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1960002	.	operator	1252	1268	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1960002	.	promoter	1269	1274	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1960002	.	operator	1275	1291	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1960002	.	engineered_region	2308	3548	.	+	0	ID=BBa_I732820;Name=BBa_I732820
BBa_K1960002	.	terminator	3508	3548	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732820
BBa_K1960002	.	stop_codon	3541	3541	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1960002	.	polyA_site	3535	3548	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1960002	.	stem_loop	3515	3534	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1960002	.	engineered_region	3508	3548	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1960002	.	terminator	3420	3499	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732820
BBa_K1960002	.	engineered_region	3420	3499	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1960002	.	stem_loop	3431	3474	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1960002	.	CDS	2326	3411	.	+	0	ID=BBa_I732100;Name=BBa_I732100;Parent=BBa_I732820
BBa_K1960002	.	ribosome_entry_site	2308	2319	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732820
BBa_K1960002	.	conserved_region	2312	2315	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1960002	.	promoter	2251	2299	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K1960002	.	engineered_region	2251	2299	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1960002	.	promoter	2288	2293	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1960002	.	non_covalent_binding_site	2275	2291	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1960002	.	promoter	2265	2270	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1960002	.	non_covalent_binding_site	2251	2267	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1960002	.	engineered_region	1	1241	.	+	0	ID=BBa_I732820;Name=BBa_I732820
BBa_K1960002	.	terminator	1201	1241	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732820
BBa_K1960002	.	stop_codon	1234	1234	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1960002	.	polyA_site	1228	1241	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1960002	.	stem_loop	1208	1227	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1960002	.	engineered_region	1201	1241	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1960002	.	terminator	1113	1192	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732820
BBa_K1960002	.	engineered_region	1113	1192	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1960002	.	stem_loop	1124	1167	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1960002	.	CDS	19	1104	.	+	0	ID=BBa_I732100;Name=BBa_I732100;Parent=BBa_I732820
BBa_K1960002	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732820
BBa_K1960002	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1960002	.	engineered_region	1313	2242	.	+	0	ID=BBa_P0451;Name=BBa_P0451
BBa_K1960002	.	ribosome_entry_site	1313	1324	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0451
BBa_K1960002	.	conserved_region	1317	1320	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1960002	.	terminator	2202	2242	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0451
BBa_K1960002	.	stop_codon	2235	2235	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1960002	.	polyA_site	2229	2242	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1960002	.	stem_loop	2209	2228	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1960002	.	engineered_region	2202	2242	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1960002	.	CDS	1331	2080	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0451
BBa_K1960002	.	engineered_tag	2081	2105	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1960002	.	CDS	1334	2041	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1960002	.	tag	2042	2074	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1960002	.	terminator	2114	2193	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0451
BBa_K1960002	.	engineered_region	2114	2193	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1960002	.	stem_loop	2125	2168	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K1960002
aaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgttt
cccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaatta
cattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctg
cacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtag
aacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaacta
tccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgac
cagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtc
accagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatct
cactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaa
atgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccatta
ccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatccc
gccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggc
caggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccg
cctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggata
acaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcacaaaaa
agaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttgg
cttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgca
ttaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagag
aaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgt
tcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaa
gccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctg
acggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatga
gtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatccca
tgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacg
aaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgata
atggttgctactagagaaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtc
tcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcga
tggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccac
ctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtg
gtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtg
ggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatt
tcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcat
ctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctg
gctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttt
tcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggc
gcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagaca
gctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgct
gcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcg
cccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaa
gcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata