LOCUS       BBa_K1960007            2265 bp    DNA     linear       26-Nov-2024
DEFINITION  Switch controller with lacI and cI
ACCESSION   BBa_K1960007
VERSION     BBa_K1960007.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..792
                     /label=cI lambda
     misc_feature    793..825
                     /label=LVA
     misc_feature    832..856
                     /label=Help:Barcodes
     CDS             82..831
                     /label=BBa_C0051
     stem_loop       872..891
                     /label=T7 TE
     misc_feature    898..898
                     /label=stop
     polyA_site      892..905
                     /label=polya
     misc_feature    865..905
                     /label=BBa_B0012
     terminator      865..905
                     /label=BBa_B0012
     stem_loop       926..948
                     /label=stem_loop
     misc_feature    914..959
                     /label=BBa_B0011
     terminator      914..959
                     /label=BBa_B0011
     misc_feature    64..959
                     /label=BBa_P0455
     misc_feature    1..959
                     /label=BBa_K1960005
     promoter        1005..1010
                     /label=-10
     promoter        982..987
                     /label=-35
     misc_feature    992..1008
                     /label=OR1
     misc_feature    968..984
                     /label=OR2
     misc_feature    968..1016
                     /label=BBa_R0051
     promoter        968..1016
                     /label=BBa_R0051
     misc_feature    1029..1032
                     /label=conserved
     RBS             1025..1036
                     /label=BBa_B0034
     CDS             1043..2128
                     /label=BBa_I732100
     stem_loop       2148..2191
                     /label=stem_loop
     misc_feature    2137..2216
                     /label=BBa_B0010
     terminator      2137..2216
                     /label=BBa_B0010
     stem_loop       2232..2251
                     /label=T7 TE
     misc_feature    2258..2258
                     /label=stop
     polyA_site      2252..2265
                     /label=polya
     misc_feature    2225..2265
                     /label=BBa_B0012
     terminator      2225..2265
                     /label=BBa_B0012
     misc_feature    1025..2265
                     /label=BBa_I732820
     misc_feature    968..2265
                     /label=BBa_K1960006
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt
      121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc
      181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat
      241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc
      301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt
      361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
      421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca
      481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca
      541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag
      601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag
      661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg
      721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa
      781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag
      841 tgctagtgta gatcgctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      901 ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt ttattatttt
      961 actagagtaa caccgtgcgt gttgactatt ttacctctgg cggtgataat ggttgctact
     1021 agagaaagag gagaaatact agatgaaacc agtaacgtta tacgatgtcg cagagtatgc
     1081 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
     1141 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
     1201 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
     1261 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
     1321 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
     1381 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
     1441 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
     1501 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
     1561 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
     1621 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
     1681 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
     1741 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
     1801 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
     1861 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
     1921 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     1981 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     2041 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     2101 ttcccgactg gaaagcgggc agtaataata ctagagccag gcatcaaata aaacgaaagg
     2161 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2221 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//