BBa_K196003
1
HfsH
HfsH protein from <i>Caulobacter crescentus</i>
2009-08-11T11:00:00Z
2015-05-08T01:11:17Z
This sequence comes from Caulobacter crescentus.
Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. Here you have HfsH.
false
false
_309_
0
4813
9
It's complicated
false
As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt.
false
Laetitia Warny
BBa_K196002
1
HfsG
HfsG protein from <i>Caulobacter crescentus</i>
2009-08-10T11:00:00Z
2015-05-08T01:11:17Z
This sequence comes from Caulobacter crescentus.
Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are differents proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we wanted this glue to be produced by Escherichia coli. As E. coli does have homologs for many of these proteins, but not for HfsG and HfsH, we decided to create a BB including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase.
false
false
_309_
0
4813
9
It's complicated
false
As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt.
false
Laetitia Warny
BBa_K196005
1
BBa_K196005
HfsG + HfsH proteins from <i>Caulobacter crescentus</i> + ter (BBa_B0015)
2009-08-12T11:00:00Z
2015-05-08T01:11:17Z
HfsG and HfsH come from Caulobacter crescentus but were synthesized by Geneart to satisfy de standard 10.
Intermediate part for the GluColi [http://2009.igem.org/Team:ULB-Brussels].
false
true
_309_
0
4813
9
It's complicated
false
-
false
Laetitia Warny
component2017276
1
BBa_B0012
component2017274
1
BBa_B0010
component2017272
1
BBa_K196002
component2017273
1
BBa_K196003
annotation2017272
1
BBa_K196002
range2017272
1
1
933
annotation2017274
1
BBa_B0010
range2017274
1
1725
1804
annotation2017273
1
BBa_K196003
range2017273
1
940
1716
annotation2017276
1
BBa_B0012
range2017276
1
1813
1853
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K196003_sequence
1
atgccgatggagttcgaaaaagtagatgcttatgaaccagaccgcagcctgaaaggcaaactgcgtcgccgtctgatccgtctggcacaccgccgtccagcaaaagtggctctggaacgtccgatggtgtctttctccttcgacgacgcgccagcaactgcttgcgaagctggcgcacgtgctctggaagctcgtggcctgcgtggcacctactatttcgctgctggtctgaccggccgtgacggccctatgggccgctatgcaactggtgaggacgcacgtcgtctgcacgaagccggtcacgaaatcgcttgccacacctactcccacctggattgtggtcagtcttctcagaccgaaaccctggctgatgtcgatcgtaatgccgaaagcctggcggcttggggtgcaggcgatccggtgtcttttgcctacccgtacggtgatgtggctgctccggctaaaacggctctgtctggtcgttttaaaactctgcgcgctctgcaccacggcctgatcaccgacggcgcagatctgaaccagactccggcagtaggcatcgaaggtgaagatggtgaaaccgttgccaaggcatggctggataaggcgaaggcacgtaaagcctggctgatcctgtatacgcacgacgtcgcaggccagcctagccagtggggttgcaccacggaagcgctggaacgcctgatcgaccgtgctctggcggacggcttcgacgttgtgacggtagccgaaggttctcgtcgtatcggcctgtaataa
BBa_K196002_sequence
1
atgaacgcccctgtaaatgaactgcgcctggagaatgctgcatgggcggcggcacagccgcgtctgtccgtgctgatccctaccttccgtgatgatccgtctgctctgctgaaggcgctggaccacaccaacgcagcggttgaagtcgtggtgctggacgatggtggcggcgacgatgctctggcggaacgcactgcgcgtcgcatcgagaaaatgcgtactccagcccgcttcgtacgtctgagccgtaacgaaggtcgtgccaaaggtcgtaaccgcctggcgagccatgctcgtggtggccacttcctgttcctggactctgacatgctgcctgacaccccggactttctggaccgttggtctgctgttgccgacaccggtgccgcggttgctttcggtggtttcaccctggaccagaccccgcagcgcccggaacatgctctgcatcgtgctatggcactgaaatctgactgcacgccggctccagaacgtgcgaaggccccggaaaaacacgttttcacgtccaacctgctggttcgtcgtgatgtattcgaaactgtgggttttgatgaaggtttctccggttggggttgggaagacgtcgaatgggccatgcgtgttgcgcgtcaacacccgatcctgcatatcgataacactgccacccacctgggcctggacccagcgccagtaatggcagccaaatatgaacaatctgccgcaaactttgcacgtgttgtcgcgagccaccgcgacgttgttagcgcatatccgtcttacaaggtagcgaaactgctgaaagcagtgccgctgatctccgtttggcgtccgctgctgaaacaggtcgcactggccgaagcggcaccggtaagcctgcgtgcattcgccatgcgtctgtaccgtgctgcgctgtacagcgaagctgtttaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K196005_sequence
1
atgaacgcccctgtaaatgaactgcgcctggagaatgctgcatgggcggcggcacagccgcgtctgtccgtgctgatccctaccttccgtgatgatccgtctgctctgctgaaggcgctggaccacaccaacgcagcggttgaagtcgtggtgctggacgatggtggcggcgacgatgctctggcggaacgcactgcgcgtcgcatcgagaaaatgcgtactccagcccgcttcgtacgtctgagccgtaacgaaggtcgtgccaaaggtcgtaaccgcctggcgagccatgctcgtggtggccacttcctgttcctggactctgacatgctgcctgacaccccggactttctggaccgttggtctgctgttgccgacaccggtgccgcggttgctttcggtggtttcaccctggaccagaccccgcagcgcccggaacatgctctgcatcgtgctatggcactgaaatctgactgcacgccggctccagaacgtgcgaaggccccggaaaaacacgttttcacgtccaacctgctggttcgtcgtgatgtattcgaaactgtgggttttgatgaaggtttctccggttggggttgggaagacgtcgaatgggccatgcgtgttgcgcgtcaacacccgatcctgcatatcgataacactgccacccacctgggcctggacccagcgccagtaatggcagccaaatatgaacaatctgccgcaaactttgcacgtgttgtcgcgagccaccgcgacgttgttagcgcatatccgtcttacaaggtagcgaaactgctgaaagcagtgccgctgatctccgtttggcgtccgctgctgaaacaggtcgcactggccgaagcggcaccggtaagcctgcgtgcattcgccatgcgtctgtaccgtgctgcgctgtacagcgaagctgtttaataatactagatgccgatggagttcgaaaaagtagatgcttatgaaccagaccgcagcctgaaaggcaaactgcgtcgccgtctgatccgtctggcacaccgccgtccagcaaaagtggctctggaacgtccgatggtgtctttctccttcgacgacgcgccagcaactgcttgcgaagctggcgcacgtgctctggaagctcgtggcctgcgtggcacctactatttcgctgctggtctgaccggccgtgacggccctatgggccgctatgcaactggtgaggacgcacgtcgtctgcacgaagccggtcacgaaatcgcttgccacacctactcccacctggattgtggtcagtcttctcagaccgaaaccctggctgatgtcgatcgtaatgccgaaagcctggcggcttggggtgcaggcgatccggtgtcttttgcctacccgtacggtgatgtggctgctccggctaaaacggctctgtctggtcgttttaaaactctgcgcgctctgcaccacggcctgatcaccgacggcgcagatctgaaccagactccggcagtaggcatcgaaggtgaagatggtgaaaccgttgccaaggcatggctggataaggcgaaggcacgtaaagcctggctgatcctgtatacgcacgacgtcgcaggccagcctagccagtggggttgcaccacggaagcgctggaacgcctgatcgaccgtgctctggcggacggcttcgacgttgtgacggtagccgaaggttctcgtcgtatcggcctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z