LOCUS       BBa_K1960054            1644 bp    DNA     linear       16-Jun-2024
DEFINITION  GFP reporter and Self-induced synthetase for AHL
ACCESSION   BBa_K1960054
VERSION     BBa_K1960054.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..795
                     /label=GFP protein
     CDS             76..795
                     /label=BBa_E0040
     stem_loop       815..858
                     /label=stem_loop
     misc_feature    804..883
                     /label=BBa_B0010
     terminator      804..883
                     /label=BBa_B0010
     stem_loop       899..918
                     /label=T7 TE
     misc_feature    925..925
                     /label=stop
     polyA_site      919..932
                     /label=polya
     misc_feature    892..932
                     /label=BBa_B0012
     terminator      892..932
                     /label=BBa_B0012
     misc_feature    1..932
                     /label=BBa_S03335
     promoter        941..975
                     /label=BBa_J23100
     misc_feature    988..991
                     /label=conserved
     RBS             984..995
                     /label=BBa_B0034
     misc_feature    1581..1612
                     /label=LVA
     CDS             1002..1580
                     /label=luxI
     misc_feature    1002..1619
                     /label=BBa_C0061
     misc_feature    1620..1644
                     /label=Help:Barcodes
     CDS             1002..1619
                     /label=BBa_C0061
     misc_feature    984..1644
                     /label=BBa_C0261
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      121 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      181 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      241 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      301 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      361 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      421 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      481 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      541 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      601 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      661 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      721 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      781 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      901 ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttgacggcta gctcagtcct
      961 aggtacagtg ctagctacta gagaaagagg agaaatacta gatgactata atgataaaaa
     1021 aatcggattt tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc
     1081 aagtgtttaa gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg
     1141 agtatgataa ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg
     1201 gatgctggcg tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat
     1261 tgcttggtca acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg
     1321 taggtaaaaa tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg
     1381 aagctatata taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa
     1441 cagcaataga gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag
     1501 aaattcatgt attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt
     1561 ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta cgctttagta gcttaataac
     1621 tctgatagtg ctagtgtaga tctc
//