##gff-version 3
##sequence-region BBa_K1960054 1 1644
BBa_K1960054	.	engineered_region	1	932	.	+	0	ID=BBa_S03335;Name=BBa_S03335
BBa_K1960054	.	terminator	892	932	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03335
BBa_K1960054	.	polyA_site	919	932	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1960054	.	engineered_region	892	932	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1960054	.	stem_loop	899	918	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1960054	.	stop_codon	925	925	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1960054	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03335
BBa_K1960054	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1960054	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_S03335
BBa_K1960054	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1960054	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1960054	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1960054	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1960054	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1960054	.	terminator	804	883	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03335
BBa_K1960054	.	stem_loop	815	858	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1960054	.	engineered_region	804	883	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1960054	.	CDS	76	795	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_S03335
BBa_K1960054	.	CDS	76	795	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1960054	.	engineered_region	984	1644	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K1960054	.	ribosome_entry_site	984	995	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1960054	.	conserved_region	988	991	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1960054	.	CDS	1002	1619	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1960054	.	CDS	1002	1580	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1960054	.	engineered_region	1002	1619	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1960054	.	tag	1581	1612	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1960054	.	engineered_tag	1620	1644	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1960054	.	promoter	941	975	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_K1960054
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgc
gtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttc
tgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacct
gttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagc
atgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaa
gacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagat
ggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatg
gaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatac
tccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaac
gaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaat
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggc
tagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgactataatgataaaaaaatcgga
ttttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgg
gacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgata
ctgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgct
tggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagata
aataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaat
atgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaaga
aattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaat
gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc