LOCUS       BBa_K196008              977 bp    DNA     linear       25-Nov-2024
DEFINITION  Lux cassette + LacI promoter + cI434 + ter
ACCESSION   BBa_K196008
VERSION     BBa_K196008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    66..82
                     /label=lac O1
     promoter        83..88
                     /label=-35
     misc_feature    89..105
                     /label=lac O1
     promoter        106..111
                     /label=-10
     misc_feature    64..117
                     /label=BBa_R0011
     promoter        64..117
                     /label=BBa_R0011
     misc_feature    133..136
                     /label=conserved
     RBS             127..140
                     /label=BBa_B0031
     CDS             147..815
                     /label=cI 434
     misc_feature    777..815
                     /label=LVA
     misc_feature    147..815
                     /label=BBa_C0052
     misc_feature    816..840
                     /label=Help:Barcodes
     CDS             147..815
                     /label=BBa_C0052
     stem_loop       860..903
                     /label=stem_loop
     misc_feature    849..928
                     /label=BBa_B0010
     terminator      849..928
                     /label=BBa_B0010
     stem_loop       944..963
                     /label=T7 TE
     misc_feature    970..970
                     /label=stop
     polyA_site      964..977
                     /label=polya
     misc_feature    937..977
                     /label=BBa_B0012
     terminator      937..977
                     /label=BBa_B0012
     misc_feature    127..977
                     /label=BBa_P0152
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
      121 ctagagtcac acaggaaacc tactagatga gtatttcttc cagggtaaaa agcaaaagaa
      181 tccagcttgg acttaaccag gctgaacttg ctcaaaaggt ggggactacc cagcagtcta
      241 tagagcagct cgaaaacggt aaaactaagc gaccacgctt tttaccagaa cttgcgtcag
      301 ctcttggcgt aagtgttgac tggctgctca atggcacctc tgattcgaat gttagatttg
      361 ttgggcacgt tgagcccaaa gggaaatatc cattgattag catggttaga gctggttcgt
      421 ggtgtgaagc ttgtgaaccc tacgatatca aggacattga tgaatggtat gacagtgacg
      481 ttaacttatt aggcaatgga ttctggctga aggttgaagg tgattccatg acctcacctg
      541 taggtcaaag catccctgaa ggtcatatgg tgttagtaga tactggacgg gagccagtga
      601 atggaagcct tgttgtagcc aaactgactg acgcgaacga agcaacattc aagaaactgg
      661 tcatagatgg cggtcagaag tacctgaaag gcctgaatcc ttcatggcct atgactccta
      721 tcaacggaaa ctgcaagatt atcggtgttg tcgtggaagc gagggtaaaa ttcgtagctg
      781 caaacgacga aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc
      841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      961 gcctttctgc gtttata
//