LOCUS       BBa_K196010              872 bp    DNA     linear       22-Nov-2024
DEFINITION  Lux cassette + c2 P22 promoter + RBS +  LuxI + ter
ACCESSION   BBa_K196010
VERSION     BBa_K196010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Lux-box
     promoter        20..25
                     /label=-35
     misc_feature    23..40
                     /label=OR2
     promoter        42..47
                     /label=-10
     misc_feature    46..63
                     /label=OR1
     promoter        1..66
                     /label=BBa_K145150
     misc_feature    79..82
                     /label=conserved
     RBS             75..86
                     /label=BBa_B0034
     misc_feature    672..703
                     /label=LVA
     CDS             93..671
                     /label=luxI
     misc_feature    93..710
                     /label=BBa_C0061
     misc_feature    711..735
                     /label=Help:Barcodes
     CDS             93..710
                     /label=BBa_C0061
     stem_loop       755..798
                     /label=stem_loop
     misc_feature    744..823
                     /label=BBa_B0010
     terminator      744..823
                     /label=BBa_B0010
     stem_loop       839..858
                     /label=T7 TE
     misc_feature    865..865
                     /label=stop
     polyA_site      859..872
                     /label=polya
     misc_feature    832..872
                     /label=BBa_B0012
     terminator      832..872
                     /label=BBa_B0012
     misc_feature    75..872
                     /label=BBa_F1610
ORIGIN
        1 acctgtagga tcgtacaggt ttactaaaga ttcctttagt ttataattta agtgttcttt
       61 aatttctact agagaaagag gagaaatact agatgactat aatgataaaa aaatcggatt
      121 ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta
      181 agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata
      241 actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc
      301 gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc
      361 aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa
      421 atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat
      481 ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag
      541 agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg
      601 tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag
      661 cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt
      721 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      781 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      841 ctcaccttcg ggtgggcctt tctgcgttta ta
//