LOCUS       BBa_K1960100            1464 bp    DNA     linear       14-Jun-2024
DEFINITION  Formaldehyde sensing device
ACCESSION   BBa_K1960100
VERSION     BBa_K1960100.1  
FEATURES             Location/Qualifiers
     RBS             579..586
                     /label=rbs
     promoter        534..570
                     /label=Formaldehyde promoter
     promoter        422..470
                     /label=weak promoter
     misc_feature    514..538
                     /label=HxlR protein binding site 2
     misc_feature    489..513
                     /label=HxlR protein binding site 1
     CDS             1..363
                     /label=HxlR coding sequence
     promoter        1..595
                     /label=BBa_K1334002
     misc_feature    608..611
                     /label=conserved
     RBS             604..615
                     /label=BBa_B0034
     CDS             622..1296
                     /label=mrfp1
     misc_feature    1303..1327
                     /label=Help:Barcodes
     CDS             622..1327
                     /label=BBa_E1010
     stem_loop       1347..1390
                     /label=stem_loop
     misc_feature    1336..1415
                     /label=BBa_B0010
     terminator      1336..1415
                     /label=BBa_B0010
     stem_loop       1431..1450
                     /label=T7 TE
     misc_feature    1457..1457
                     /label=stop
     polyA_site      1451..1464
                     /label=polya
     misc_feature    1424..1464
                     /label=BBa_B0012
     terminator      1424..1464
                     /label=BBa_B0012
     terminator      1336..1464
                     /label=BBa_B0015
     misc_feature    604..1464
                     /label=BBa_K1357010
ORIGIN
        1 ttacaacgat tctttcatga catttttgtc gatatcaatc aattccatat agcctttccc
       61 ccactcatac atggcttcaa gaataggcat gaggctttct ccgtgcgggg tcagagaata
      121 ttcaaccttc ggcgggacaa ctggatacac ttccctgtga acaatcatat cctgctcaag
      181 ctctctcagc tgattcacga ggatcttctg cgtaatatca ggaatcaatg tttttaattc
      241 attgaaccgt tttgtgcctt cttttcctaa atgccataaa atgagcattt tccatttacc
      301 gccaatcact gcaagcgtta attccttctc acaattaaac cttttgtcgt ccatccggct
      361 caatagaatc ccccccttag taaccttttt gtatgttaga tacgtatcat cacttactta
      421 cttttttgca gactcttttt tcattatact cactcataaa atcaagcgaa agaaatctga
      481 ttgtctctcc tcacagtatc ctccaagtaa cttgttgact tcaaagtgcc tacttctcaa
      541 caaaagaagg aacctataga atgaacaata attcgaaaaa gggagtggat caacatacta
      601 gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt
      661 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
      721 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
      781 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
      841 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
      901 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg
      961 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt
     1021 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
     1081 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
     1141 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
     1201 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag
     1261 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt
     1321 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1381 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1441 cgggtgggcc tttctgcgtt tata
//