LOCUS BBa_K1960104 3354 bp DNA linear 14-Nov-2024 DEFINITION Formaldehyde reporting device ACCESSION BBa_K1960104 VERSION BBa_K1960104.1 FEATURES Location/Qualifiers RBS 579..586 /label=rbs promoter 534..570 /label=Formaldehyde promoter promoter 422..470 /label=weak promoter misc_feature 514..538 /label=HxlR protein binding site 2 misc_feature 489..513 /label=HxlR protein binding site 1 CDS 1..363 /label=HxlR coding sequence promoter 1..595 /label=BBa_K1334002 misc_feature 608..611 /label=conserved RBS 604..615 /label=BBa_B0034 misc_feature 1201..1232 /label=LVA CDS 622..1200 /label=luxI misc_feature 622..1239 /label=BBa_C0061 misc_feature 1240..1264 /label=Help:Barcodes CDS 622..1239 /label=BBa_C0061 stem_loop 1284..1327 /label=stem_loop misc_feature 1273..1352 /label=BBa_B0010 terminator 1273..1352 /label=BBa_B0010 stem_loop 1368..1387 /label=T7 TE misc_feature 1394..1394 /label=stop polyA_site 1388..1401 /label=polya misc_feature 1361..1401 /label=BBa_B0012 terminator 1361..1401 /label=BBa_B0012 terminator 1273..1401 /label=BBa_B0015 misc_feature 604..1401 /label=BBa_K805016 misc_feature 1410..1428 /label=TetR 1 misc_feature 1410..1463 /label=BBa_R0040 promoter 1429..1434 /label=-35 misc_feature 1435..1453 /label=TetR 2 promoter 1452..1457 /label=-10 promoter 1410..1463 /label=BBa_R0040 misc_feature 1476..1479 /label=conserved RBS 1472..1483 /label=BBa_B0034 misc_feature 1490..1491 /label=prefix misc_feature 1663..1663 /label=T misc_feature 1981..1981 /label=A CDS 1490..2239 /label=luxR misc_feature 1490..2245 /label=BBa_C0062 misc_feature 2246..2270 /label=Help:Barcodes CDS 1490..2245 /label=BBa_C0062 stem_loop 2290..2333 /label=stem_loop misc_feature 2279..2358 /label=BBa_B0010 terminator 2279..2358 /label=BBa_B0010 stem_loop 2374..2393 /label=T7 TE misc_feature 2400..2400 /label=stop polyA_site 2394..2407 /label=polya misc_feature 2367..2407 /label=BBa_B0012 terminator 2367..2407 /label=BBa_B0012 misc_feature 2416..2435 /label=LuxR/HSL promoter 2435..2440 /label=-35 promoter 2457..2462 /label=-10 misc_feature 2468..2468 /label=start misc_feature 2416..2470 /label=BBa_R0062 promoter 2416..2470 /label=BBa_R0062 RBS 2479..2491 /label=RBS-3\Weak RBS 2485..2488 /label=RBS misc_feature 2479..2491 /label=BBa_B0032 RBS 2479..2491 /label=BBa_B0032 CDS 2498..3217 /label=GFP protein CDS 2498..3217 /label=BBa_E0040 stem_loop 3237..3280 /label=stem_loop misc_feature 3226..3305 /label=BBa_B0010 terminator 3226..3305 /label=BBa_B0010 stem_loop 3321..3340 /label=T7 TE misc_feature 3347..3347 /label=stop polyA_site 3341..3354 /label=polya misc_feature 3314..3354 /label=BBa_B0012 terminator 3314..3354 /label=BBa_B0012 misc_feature 1410..3354 /label=BBa_T9002 ORIGIN 1 ttacaacgat tctttcatga catttttgtc gatatcaatc aattccatat agcctttccc 61 ccactcatac atggcttcaa gaataggcat gaggctttct ccgtgcgggg tcagagaata 121 ttcaaccttc ggcgggacaa ctggatacac ttccctgtga acaatcatat cctgctcaag 181 ctctctcagc tgattcacga ggatcttctg cgtaatatca ggaatcaatg tttttaattc 241 attgaaccgt tttgtgcctt cttttcctaa atgccataaa atgagcattt tccatttacc 301 gccaatcact gcaagcgtta attccttctc acaattaaac cttttgtcgt ccatccggct 361 caatagaatc ccccccttag taaccttttt gtatgttaga tacgtatcat cacttactta 421 cttttttgca gactcttttt tcattatact cactcataaa atcaagcgaa agaaatctga 481 ttgtctctcc tcacagtatc ctccaagtaa cttgttgact tcaaagtgcc tacttctcaa 541 caaaagaagg aacctataga atgaacaata attcgaaaaa gggagtggat caacatacta 601 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt 661 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 721 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 781 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 841 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 901 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 961 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 1021 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 1081 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 1141 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 1201 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga 1261 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 1321 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 1381 gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta 1441 tcagtgatag agatactgag cactactaga gaaagaggag aaatactaga tgaaaaacat 1501 aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtagaa gcaataatga 1561 tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt atttactcgc 1621 gatcatttat cctcattcta tggttaaatc tgatatttca atcctagata attaccctaa 1681 aaaatggagg caatattatg atgacgctaa tttaataaaa tatgatccta tagtagatta 1741 ttctaactcc aatcattcac caattaattg gaatatattt gaaaacaatg ctgtaaataa 1801 aaaatctcca aatgtaatta aagaagcgaa aacatcaggt cttatcactg ggtttagttt 1861 ccctattcat acggctaaca atggcttcgg aatgcttagt tttgcacatt cagaaaaaga 1921 caactatata gatagtttat ttttacatgc gtgtatgaac ataccattaa ttgttccttc 1981 tctagttgat aattatcgaa aaataaatat agcaaataat aaatcaaaca acgatttaac 2041 caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt gggatatttc 2101 aaaaatatta ggttgcagtg agcgtactgt cactttccat ttaaccaatg cgcaaatgaa 2161 actcaataca acaaaccgct gccaaagtat ttctaaagca attttaacag gagcaattga 2221 ttgcccatac tttaaaaatt aataacactg atagtgctag tgtagatcac tactagagcc 2281 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 2341 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 2401 gtttatatac tagagacctg taggatcgta caggtttacg caagaaaatg gtttgttata 2461 gtcgaataaa tactagagtc acacaggaaa gtactagatg cgtaaaggag aagaactttt 2521 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc 2581 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg 2641 cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt 2701 tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat 2761 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac 2821 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat 2881 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca 2941 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag 3001 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat 3061 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc 3121 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg 3181 gattacacat ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa 3241 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 3301 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //