##gff-version 3
##sequence-region BBa_K1960104 1 3354
BBa_K1960104	.	engineered_region	604	1401	.	+	0	ID=BBa_K805016;Name=BBa_K805016
BBa_K1960104	.	CDS	622	1239	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K805016
BBa_K1960104	.	CDS	622	1200	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1960104	.	engineered_region	622	1239	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1960104	.	tag	1201	1232	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1960104	.	engineered_tag	1240	1264	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1960104	.	terminator	1273	1401	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K805016
BBa_K1960104	.	terminator	1273	1352	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1960104	.	engineered_region	1273	1352	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1960104	.	stem_loop	1284	1327	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1960104	.	terminator	1361	1401	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1960104	.	polyA_site	1388	1401	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1960104	.	stop_codon	1394	1394	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1960104	.	engineered_region	1361	1401	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1960104	.	stem_loop	1368	1387	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1960104	.	ribosome_entry_site	604	615	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K805016
BBa_K1960104	.	conserved_region	608	611	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1960104	.	engineered_region	1410	3354	.	+	0	ID=BBa_T9002;Name=BBa_T9002
BBa_K1960104	.	ribosome_entry_site	1472	1483	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_T9002
BBa_K1960104	.	conserved_region	1476	1479	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1960104	.	promoter	1410	1463	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_T9002
BBa_K1960104	.	engineered_region	1410	1463	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1960104	.	non_covalent_binding_site	1410	1428	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1960104	.	promoter	1452	1457	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1960104	.	non_covalent_binding_site	1435	1453	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1960104	.	promoter	1429	1434	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1960104	.	terminator	2279	2358	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_T9002
BBa_K1960104	.	engineered_region	2279	2358	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1960104	.	stem_loop	2290	2333	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1960104	.	terminator	2367	2407	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_T9002
BBa_K1960104	.	polyA_site	2394	2407	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1960104	.	stop_codon	2400	2400	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1960104	.	engineered_region	2367	2407	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1960104	.	stem_loop	2374	2393	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1960104	.	CDS	1490	2245	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_T9002
BBa_K1960104	.	engineered_tag	2246	2270	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1960104	.	point_mutation	1981	1981	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1960104	.	engineered_region	1490	1491	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1960104	.	engineered_region	1490	2245	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1960104	.	CDS	1490	2239	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1960104	.	point_mutation	1663	1663	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1960104	.	terminator	3314	3354	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_T9002
BBa_K1960104	.	polyA_site	3341	3354	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1960104	.	stop_codon	3347	3347	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1960104	.	engineered_region	3314	3354	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1960104	.	stem_loop	3321	3340	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1960104	.	CDS	2498	3217	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_T9002
BBa_K1960104	.	CDS	2498	3217	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1960104	.	promoter	2416	2470	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_T9002
BBa_K1960104	.	start_codon	2468	2468	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1960104	.	promoter	2457	2462	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1960104	.	engineered_region	2416	2470	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1960104	.	promoter	2435	2440	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1960104	.	non_covalent_binding_site	2416	2435	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1960104	.	terminator	3226	3305	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_T9002
BBa_K1960104	.	engineered_region	3226	3305	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1960104	.	stem_loop	3237	3280	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1960104	.	ribosome_entry_site	2479	2491	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_T9002
BBa_K1960104	.	ribosome_entry_site	2479	2491	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1960104	.	engineered_region	2479	2491	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1960104	.	ribosome_entry_site	2485	2488	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1960104	.	promoter	1	595	.	+	0	ID=BBa_K1334002;Name=BBa_K1334002
BBa_K1960104	.	CDS	1	363	.	+	0	ID=annotation2387313;Name=HxlR coding sequence;Parent=BBa_K1334002
BBa_K1960104	.	non_covalent_binding_site	514	538	.	+	0	ID=annotation2387311;Name=HxlR protein binding site 2;Parent=BBa_K1334002
BBa_K1960104	.	non_covalent_binding_site	489	513	.	+	0	ID=annotation2387312;Name=HxlR protein binding site 1;Parent=BBa_K1334002
BBa_K1960104	.	ribosome_entry_site	579	586	.	+	0	ID=annotation2387242;Name=rbs;Parent=BBa_K1334002
BBa_K1960104	.	promoter	534	570	.	+	0	ID=annotation2387265;Name=Formaldehyde promoter;Parent=BBa_K1334002
BBa_K1960104	.	promoter	422	470	.	+	0	ID=annotation2387310;Name=weak promoter;Parent=BBa_K1334002
>BBa_K1960104
ttacaacgattctttcatgacatttttgtcgatatcaatcaattccatatagcctttcccccactcatacatggcttca
agaataggcatgaggctttctccgtgcggggtcagagaatattcaaccttcggcgggacaactggatacacttccctgt
gaacaatcatatcctgctcaagctctctcagctgattcacgaggatcttctgcgtaatatcaggaatcaatgtttttaa
ttcattgaaccgttttgtgccttcttttcctaaatgccataaaatgagcattttccatttaccgccaatcactgcaagc
gttaattccttctcacaattaaaccttttgtcgtccatccggctcaatagaatcccccccttagtaacctttttgtatg
ttagatacgtatcatcacttacttacttttttgcagactcttttttcattatactcactcataaaatcaagcgaaagaa
atctgattgtctctcctcacagtatcctccaagtaacttgttgacttcaaagtgcctacttctcaacaaaagaaggaac
ctatagaatgaacaataattcgaaaaagggagtggatcaacatactagagaaagaggagaaatactagatgactataat
gataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaag
caaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatattt
atgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgt
ttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaa
aatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtc
aaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcg
tattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaa
aaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtga
tagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacata
aatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctg
atatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttc
aatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagat
tattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaa
ttaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgct
tagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgtt
ccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaag
aatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcac
tttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacagga
gcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaat
ggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggag
ttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgc
aacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactact
ttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccg
aaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaagg
tgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaa
tacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagac
acaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttt
accagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttctt
gagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata