LOCUS       BBa_K196012             2286 bp    DNA     linear       27-Dec-2024
DEFINITION  Glue synthesizer with repressed replication
ACCESSION   BBa_K196012
VERSION     BBa_K196012.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Lux-box
     promoter        20..25
                     /label=-35
     misc_feature    23..40
                     /label=OR2
     promoter        42..47
                     /label=-10
     misc_feature    46..63
                     /label=OR1
     promoter        1..66
                     /label=BBa_K145150
     misc_feature    79..82
                     /label=conserved
     RBS             75..86
                     /label=BBa_B0034
     CDS             93..1025
                     /label=BBa_K196002
     CDS             1032..1808
                     /label=BBa_K196003
     CDS             1817..2149
                     /label=BBa_I716211
     stem_loop       2169..2212
                     /label=stem_loop
     misc_feature    2158..2237
                     /label=BBa_B0010
     terminator      2158..2237
                     /label=BBa_B0010
     stem_loop       2253..2272
                     /label=T7 TE
     misc_feature    2279..2279
                     /label=stop
     polyA_site      2273..2286
                     /label=polya
     misc_feature    2246..2286
                     /label=BBa_B0012
     terminator      2246..2286
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttactaaaga ttcctttagt ttataattta agtgttcttt
       61 aatttctact agagaaagag gagaaatact agatgaacgc ccctgtaaat gaactgcgcc
      121 tggagaatgc tgcatgggcg gcggcacagc cgcgtctgtc cgtgctgatc cctaccttcc
      181 gtgatgatcc gtctgctctg ctgaaggcgc tggaccacac caacgcagcg gttgaagtcg
      241 tggtgctgga cgatggtggc ggcgacgatg ctctggcgga acgcactgcg cgtcgcatcg
      301 agaaaatgcg tactccagcc cgcttcgtac gtctgagccg taacgaaggt cgtgccaaag
      361 gtcgtaaccg cctggcgagc catgctcgtg gtggccactt cctgttcctg gactctgaca
      421 tgctgcctga caccccggac tttctggacc gttggtctgc tgttgccgac accggtgccg
      481 cggttgcttt cggtggtttc accctggacc agaccccgca gcgcccggaa catgctctgc
      541 atcgtgctat ggcactgaaa tctgactgca cgccggctcc agaacgtgcg aaggccccgg
      601 aaaaacacgt tttcacgtcc aacctgctgg ttcgtcgtga tgtattcgaa actgtgggtt
      661 ttgatgaagg tttctccggt tggggttggg aagacgtcga atgggccatg cgtgttgcgc
      721 gtcaacaccc gatcctgcat atcgataaca ctgccaccca cctgggcctg gacccagcgc
      781 cagtaatggc agccaaatat gaacaatctg ccgcaaactt tgcacgtgtt gtcgcgagcc
      841 accgcgacgt tgttagcgca tatccgtctt acaaggtagc gaaactgctg aaagcagtgc
      901 cgctgatctc cgtttggcgt ccgctgctga aacaggtcgc actggccgaa gcggcaccgg
      961 taagcctgcg tgcattcgcc atgcgtctgt accgtgctgc gctgtacagc gaagctgttt
     1021 aataatacta gatgccgatg gagttcgaaa aagtagatgc ttatgaacca gaccgcagcc
     1081 tgaaaggcaa actgcgtcgc cgtctgatcc gtctggcaca ccgccgtcca gcaaaagtgg
     1141 ctctggaacg tccgatggtg tctttctcct tcgacgacgc gccagcaact gcttgcgaag
     1201 ctggcgcacg tgctctggaa gctcgtggcc tgcgtggcac ctactatttc gctgctggtc
     1261 tgaccggccg tgacggccct atgggccgct atgcaactgg tgaggacgca cgtcgtctgc
     1321 acgaagccgg tcacgaaatc gcttgccaca cctactccca cctggattgt ggtcagtctt
     1381 ctcagaccga aaccctggct gatgtcgatc gtaatgccga aagcctggcg gcttggggtg
     1441 caggcgatcc ggtgtctttt gcctacccgt acggtgatgt ggctgctccg gctaaaacgg
     1501 ctctgtctgg tcgttttaaa actctgcgcg ctctgcacca cggcctgatc accgacggcg
     1561 cagatctgaa ccagactccg gcagtaggca tcgaaggtga agatggtgaa accgttgcca
     1621 aggcatggct ggataaggcg aaggcacgta aagcctggct gatcctgtat acgcacgacg
     1681 tcgcaggcca gcctagccag tggggttgca ccacggaagc gctggaacgc ctgatcgacc
     1741 gtgctctggc ggacggcttc gacgttgtga cggtagccga aggttctcgt cgtatcggcc
     1801 tgtaataata ctagaggcac aggttatcaa cacgtttgac ggggttgcgg attatcttca
     1861 gacatatcat aagctacctg ataattacat tacaaaatca gaagcacaag ccctcggctg
     1921 ggtggcatca aaagggaacc ttgcagacgt cgctccgggg aaaagcatcg gcggagacat
     1981 cttctcaaac agggaaggca aactcccggg caaaagcgga cgaacatggc gtgaagcgga
     2041 tattaactat acatcaggct tcagaaattc agaccggatt ctttactcaa gcgactggct
     2101 gatttacaaa acaacggacc attatcagac ctttacaaaa atcagataat actagagcca
     2161 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2221 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     2281 tttata
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