BBa_K196003 1 HfsH HfsH protein from <i>Caulobacter crescentus</i> 2009-08-11T11:00:00Z 2015-05-08T01:11:17Z This sequence comes from Caulobacter crescentus. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. Here you have HfsH. false false _309_ 0 4813 9 It's complicated false As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt. false Laetitia Warny BBa_K196012 1 BBa_K196012 Glue synthesizer with repressed replication 2009-08-19T11:00:00Z 2015-05-08T01:11:17Z To be completed. To be completed. false false _309_ 0 4813 9 Not in stock false To be completed. false Laetitia Warny component2053319 1 BBa_B0010 component2053316 1 BBa_K196002 component2053313 1 BBa_K145150 component2053317 1 BBa_K196003 component2053318 1 BBa_I716211 component2053321 1 BBa_B0012 component2053315 1 BBa_B0034 annotation2053313 1 BBa_K145150 range2053313 1 1 66 annotation2053315 1 BBa_B0034 range2053315 1 75 86 annotation2053317 1 BBa_K196003 range2053317 1 1032 1808 annotation2053321 1 BBa_B0012 range2053321 1 2246 2286 annotation2053318 1 BBa_I716211 range2053318 1 1817 2149 annotation2053316 1 BBa_K196002 range2053316 1 93 1025 annotation2053319 1 BBa_B0010 range2053319 1 2158 2237 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K196002 1 HfsG HfsG protein from <i>Caulobacter crescentus</i> 2009-08-10T11:00:00Z 2015-05-08T01:11:17Z This sequence comes from Caulobacter crescentus. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are differents proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we wanted this glue to be produced by Escherichia coli. As E. coli does have homologs for many of these proteins, but not for HfsG and HfsH, we decided to create a BB including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. false false _309_ 0 4813 9 It's complicated false As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt. false Laetitia Warny BBa_I716211 1 BBa_I716211 Barnase active peptide 2007-06-04T11:00:00Z 2015-08-31T04:07:51Z PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716211 sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg Released HQ 2013 Barnase active peptide (no start codon) false true _110_ 0 933 52 In stock false PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716211 sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg false Samantha Liang BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K145150 1 BBa_K145150 Hybrid promoter: HSL-LuxR activated, P22 C2 repressed 2008-08-05T11:00:00Z 2015-05-08T01:10:29Z Synthetic Hybrid promoter consisting of the Lux box which overlaps partly with -35 box. Binding sites for P22 C2 O_R2 and O_R1 are located between the -35 and -10 boxes and downstream of them respectively. false false _257_ 0 2970 9 It's complicated false -35 box is partly composite. -10 is taken from the P22 phage P_R regulatory region true Jonas Demeulemeester annotation1971582 1 Lux-box range1971582 1 1 20 annotation1971584 1 OR2 range1971584 1 23 40 annotation1971585 1 -10 range1971585 1 42 47 annotation1971583 1 -35 range1971583 1 20 25 annotation1971586 1 OR1 range1971586 1 46 63 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K196003_sequence 1 atgccgatggagttcgaaaaagtagatgcttatgaaccagaccgcagcctgaaaggcaaactgcgtcgccgtctgatccgtctggcacaccgccgtccagcaaaagtggctctggaacgtccgatggtgtctttctccttcgacgacgcgccagcaactgcttgcgaagctggcgcacgtgctctggaagctcgtggcctgcgtggcacctactatttcgctgctggtctgaccggccgtgacggccctatgggccgctatgcaactggtgaggacgcacgtcgtctgcacgaagccggtcacgaaatcgcttgccacacctactcccacctggattgtggtcagtcttctcagaccgaaaccctggctgatgtcgatcgtaatgccgaaagcctggcggcttggggtgcaggcgatccggtgtcttttgcctacccgtacggtgatgtggctgctccggctaaaacggctctgtctggtcgttttaaaactctgcgcgctctgcaccacggcctgatcaccgacggcgcagatctgaaccagactccggcagtaggcatcgaaggtgaagatggtgaaaccgttgccaaggcatggctggataaggcgaaggcacgtaaagcctggctgatcctgtatacgcacgacgtcgcaggccagcctagccagtggggttgcaccacggaagcgctggaacgcctgatcgaccgtgctctggcggacggcttcgacgttgtgacggtagccgaaggttctcgtcgtatcggcctgtaataa BBa_I716211_sequence 1 gcacaggttatcaacacgtttgacggggttgcggattatcttcagacatatcataagctacctgataattacattacaaaatcagaagcacaagccctcggctgggtggcatcaaaagggaaccttgcagacgtcgctccggggaaaagcatcggcggagacatcttctcaaacagggaaggcaaactcccgggcaaaagcggacgaacatggcgtgaagcggatattaactatacatcaggcttcagaaattcagaccggattctttactcaagcgactggctgatttacaaaacaacggaccattatcagacctttacaaaaatcagataa BBa_K196002_sequence 1 atgaacgcccctgtaaatgaactgcgcctggagaatgctgcatgggcggcggcacagccgcgtctgtccgtgctgatccctaccttccgtgatgatccgtctgctctgctgaaggcgctggaccacaccaacgcagcggttgaagtcgtggtgctggacgatggtggcggcgacgatgctctggcggaacgcactgcgcgtcgcatcgagaaaatgcgtactccagcccgcttcgtacgtctgagccgtaacgaaggtcgtgccaaaggtcgtaaccgcctggcgagccatgctcgtggtggccacttcctgttcctggactctgacatgctgcctgacaccccggactttctggaccgttggtctgctgttgccgacaccggtgccgcggttgctttcggtggtttcaccctggaccagaccccgcagcgcccggaacatgctctgcatcgtgctatggcactgaaatctgactgcacgccggctccagaacgtgcgaaggccccggaaaaacacgttttcacgtccaacctgctggttcgtcgtgatgtattcgaaactgtgggttttgatgaaggtttctccggttggggttgggaagacgtcgaatgggccatgcgtgttgcgcgtcaacacccgatcctgcatatcgataacactgccacccacctgggcctggacccagcgccagtaatggcagccaaatatgaacaatctgccgcaaactttgcacgtgttgtcgcgagccaccgcgacgttgttagcgcatatccgtcttacaaggtagcgaaactgctgaaagcagtgccgctgatctccgtttggcgtccgctgctgaaacaggtcgcactggccgaagcggcaccggtaagcctgcgtgcattcgccatgcgtctgtaccgtgctgcgctgtacagcgaagctgtttaataa BBa_K145150_sequence 1 acctgtaggatcgtacaggtttactaaagattcctttagtttataatttaagtgttctttaatttc BBa_K196012_sequence 1 acctgtaggatcgtacaggtttactaaagattcctttagtttataatttaagtgttctttaatttctactagagaaagaggagaaatactagatgaacgcccctgtaaatgaactgcgcctggagaatgctgcatgggcggcggcacagccgcgtctgtccgtgctgatccctaccttccgtgatgatccgtctgctctgctgaaggcgctggaccacaccaacgcagcggttgaagtcgtggtgctggacgatggtggcggcgacgatgctctggcggaacgcactgcgcgtcgcatcgagaaaatgcgtactccagcccgcttcgtacgtctgagccgtaacgaaggtcgtgccaaaggtcgtaaccgcctggcgagccatgctcgtggtggccacttcctgttcctggactctgacatgctgcctgacaccccggactttctggaccgttggtctgctgttgccgacaccggtgccgcggttgctttcggtggtttcaccctggaccagaccccgcagcgcccggaacatgctctgcatcgtgctatggcactgaaatctgactgcacgccggctccagaacgtgcgaaggccccggaaaaacacgttttcacgtccaacctgctggttcgtcgtgatgtattcgaaactgtgggttttgatgaaggtttctccggttggggttgggaagacgtcgaatgggccatgcgtgttgcgcgtcaacacccgatcctgcatatcgataacactgccacccacctgggcctggacccagcgccagtaatggcagccaaatatgaacaatctgccgcaaactttgcacgtgttgtcgcgagccaccgcgacgttgttagcgcatatccgtcttacaaggtagcgaaactgctgaaagcagtgccgctgatctccgtttggcgtccgctgctgaaacaggtcgcactggccgaagcggcaccggtaagcctgcgtgcattcgccatgcgtctgtaccgtgctgcgctgtacagcgaagctgtttaataatactagatgccgatggagttcgaaaaagtagatgcttatgaaccagaccgcagcctgaaaggcaaactgcgtcgccgtctgatccgtctggcacaccgccgtccagcaaaagtggctctggaacgtccgatggtgtctttctccttcgacgacgcgccagcaactgcttgcgaagctggcgcacgtgctctggaagctcgtggcctgcgtggcacctactatttcgctgctggtctgaccggccgtgacggccctatgggccgctatgcaactggtgaggacgcacgtcgtctgcacgaagccggtcacgaaatcgcttgccacacctactcccacctggattgtggtcagtcttctcagaccgaaaccctggctgatgtcgatcgtaatgccgaaagcctggcggcttggggtgcaggcgatccggtgtcttttgcctacccgtacggtgatgtggctgctccggctaaaacggctctgtctggtcgttttaaaactctgcgcgctctgcaccacggcctgatcaccgacggcgcagatctgaaccagactccggcagtaggcatcgaaggtgaagatggtgaaaccgttgccaaggcatggctggataaggcgaaggcacgtaaagcctggctgatcctgtatacgcacgacgtcgcaggccagcctagccagtggggttgcaccacggaagcgctggaacgcctgatcgaccgtgctctggcggacggcttcgacgttgtgacggtagccgaaggttctcgtcgtatcggcctgtaataatactagaggcacaggttatcaacacgtttgacggggttgcggattatcttcagacatatcataagctacctgataattacattacaaaatcagaagcacaagccctcggctgggtggcatcaaaagggaaccttgcagacgtcgctccggggaaaagcatcggcggagacatcttctcaaacagggaaggcaaactcccgggcaaaagcggacgaacatggcgtgaagcggatattaactatacatcaggcttcagaaattcagaccggattctttactcaagcgactggctgatttacaaaacaacggaccattatcagacctttacaaaaatcagataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z