LOCUS       BBa_K196013             1010 bp    DNA     linear       17-May-2024
DEFINITION  Hybrid promoter (Lux cassette + c2 P22 promoter) + RBS + LuxR + ter
ACCESSION   BBa_K196013
VERSION     BBa_K196013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Lux-box
     promoter        20..25
                     /label=-35
     misc_feature    23..40
                     /label=OR2
     promoter        42..47
                     /label=-10
     misc_feature    46..63
                     /label=OR1
     promoter        1..66
                     /label=BBa_K145150
     misc_feature    79..82
                     /label=conserved
     RBS             75..86
                     /label=BBa_B0034
     misc_feature    93..94
                     /label=prefix
     misc_feature    266..266
                     /label=T
     misc_feature    584..584
                     /label=A
     CDS             93..842
                     /label=luxR
     misc_feature    93..848
                     /label=BBa_C0062
     misc_feature    849..873
                     /label=Help:Barcodes
     CDS             93..848
                     /label=BBa_C0062
     stem_loop       893..936
                     /label=stem_loop
     misc_feature    882..961
                     /label=BBa_B0010
     terminator      882..961
                     /label=BBa_B0010
     stem_loop       977..996
                     /label=T7 TE
     misc_feature    1003..1003
                     /label=stop
     polyA_site      997..1010
                     /label=polya
     misc_feature    970..1010
                     /label=BBa_B0012
     terminator      970..1010
                     /label=BBa_B0012
     misc_feature    75..1010
                     /label=BBa_I0462
ORIGIN
        1 acctgtagga tcgtacaggt ttactaaaga ttcctttagt ttataattta agtgttcttt
       61 aatttctact agagaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat
      121 acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat
      181 ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt
      241 ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt
      301 atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt
      361 caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa
      421 ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta
      481 acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt
      541 tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc
      601 gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat
      661 gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca
      721 gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc
      781 gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa
      841 attaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg
      901 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      961 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//