LOCUS       BBa_K196014             2646 bp    DNA     linear       25-Nov-2024
DEFINITION  Final GluColi device.
ACCESSION   BBa_K196014
VERSION     BBa_K196014.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..756
                     /label=mrfp1
     misc_feature    763..787
                     /label=Help:Barcodes
     CDS             82..787
                     /label=BBa_E1010
     misc_feature    64..787
                     /label=BBa_K093005
     misc_feature    1..787
                     /label=BBa_K196007
     CDS             794..1726
                     /label=BBa_K196002
     CDS             1733..2509
                     /label=BBa_K196003
     stem_loop       2529..2572
                     /label=stem_loop
     misc_feature    2518..2597
                     /label=BBa_B0010
     terminator      2518..2597
                     /label=BBa_B0010
     stem_loop       2613..2632
                     /label=T7 TE
     misc_feature    2639..2639
                     /label=stop
     polyA_site      2633..2646
                     /label=polya
     misc_feature    2606..2646
                     /label=BBa_B0012
     terminator      2606..2646
                     /label=BBa_B0012
     misc_feature    794..2646
                     /label=BBa_K196005
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt
      121 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
      181 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
      241 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
      301 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
      361 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg
      421 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt
      481 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
      541 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
      601 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
      661 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag
      721 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt
      781 agatcgctac tagatgaacg cccctgtaaa tgaactgcgc ctggagaatg ctgcatgggc
      841 ggcggcacag ccgcgtctgt ccgtgctgat ccctaccttc cgtgatgatc cgtctgctct
      901 gctgaaggcg ctggaccaca ccaacgcagc ggttgaagtc gtggtgctgg acgatggtgg
      961 cggcgacgat gctctggcgg aacgcactgc gcgtcgcatc gagaaaatgc gtactccagc
     1021 ccgcttcgta cgtctgagcc gtaacgaagg tcgtgccaaa ggtcgtaacc gcctggcgag
     1081 ccatgctcgt ggtggccact tcctgttcct ggactctgac atgctgcctg acaccccgga
     1141 ctttctggac cgttggtctg ctgttgccga caccggtgcc gcggttgctt tcggtggttt
     1201 caccctggac cagaccccgc agcgcccgga acatgctctg catcgtgcta tggcactgaa
     1261 atctgactgc acgccggctc cagaacgtgc gaaggccccg gaaaaacacg ttttcacgtc
     1321 caacctgctg gttcgtcgtg atgtattcga aactgtgggt tttgatgaag gtttctccgg
     1381 ttggggttgg gaagacgtcg aatgggccat gcgtgttgcg cgtcaacacc cgatcctgca
     1441 tatcgataac actgccaccc acctgggcct ggacccagcg ccagtaatgg cagccaaata
     1501 tgaacaatct gccgcaaact ttgcacgtgt tgtcgcgagc caccgcgacg ttgttagcgc
     1561 atatccgtct tacaaggtag cgaaactgct gaaagcagtg ccgctgatct ccgtttggcg
     1621 tccgctgctg aaacaggtcg cactggccga agcggcaccg gtaagcctgc gtgcattcgc
     1681 catgcgtctg taccgtgctg cgctgtacag cgaagctgtt taataatact agatgccgat
     1741 ggagttcgaa aaagtagatg cttatgaacc agaccgcagc ctgaaaggca aactgcgtcg
     1801 ccgtctgatc cgtctggcac accgccgtcc agcaaaagtg gctctggaac gtccgatggt
     1861 gtctttctcc ttcgacgacg cgccagcaac tgcttgcgaa gctggcgcac gtgctctgga
     1921 agctcgtggc ctgcgtggca cctactattt cgctgctggt ctgaccggcc gtgacggccc
     1981 tatgggccgc tatgcaactg gtgaggacgc acgtcgtctg cacgaagccg gtcacgaaat
     2041 cgcttgccac acctactccc acctggattg tggtcagtct tctcagaccg aaaccctggc
     2101 tgatgtcgat cgtaatgccg aaagcctggc ggcttggggt gcaggcgatc cggtgtcttt
     2161 tgcctacccg tacggtgatg tggctgctcc ggctaaaacg gctctgtctg gtcgttttaa
     2221 aactctgcgc gctctgcacc acggcctgat caccgacggc gcagatctga accagactcc
     2281 ggcagtaggc atcgaaggtg aagatggtga aaccgttgcc aaggcatggc tggataaggc
     2341 gaaggcacgt aaagcctggc tgatcctgta tacgcacgac gtcgcaggcc agcctagcca
     2401 gtggggttgc accacggaag cgctggaacg cctgatcgac cgtgctctgg cggacggctt
     2461 cgacgttgtg acggtagccg aaggttctcg tcgtatcggc ctgtaataat actagagcca
     2521 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2581 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     2641 tttata
//