BBa_K196014 1 GluColi Final GluColi device. 2009-10-17T11:00:00Z 2015-05-08T01:11:17Z HfsG and HfsH sequences come from Caulobacter crescentus. This is the part we actually created to test the glue production by E. coli. HfsG and HfsH come from Caulobacter crescentu. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see our wiki page [1]. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. RFP has been added as a reporter. false false _309_ 0 4813 9 It's complicated true As many mutations were needed to make the part compatible with the standard 10, we decided to make hfsG and hfsH synthetized by GeneArt [2]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli. false Laetitia Warny component2267888 1 BBa_K196005 component2267879 1 BBa_K196007 annotation2267879 1 BBa_K196007 range2267879 1 1 787 annotation2267888 1 BBa_K196005 range2267888 1 794 2646 BBa_K196007 1 BBa_K196007 Promoter (lacI regulated, lambda pL hybrid) + RFP + RBS (GluColi project) 2009-08-12T11:00:00Z 2015-05-08T01:11:17Z - Intermediate in the GluColi project in order to have a construction with a reporter. false false _309_ 0 4813 9 It's complicated false - false Laetitia Warny component2246510 1 BBa_K093005 component2246500 1 BBa_R0011 annotation2246500 1 BBa_R0011 range2246500 1 1 54 annotation2246510 1 BBa_K093005 range2246510 1 64 787 BBa_K093005 1 RBS-RFP RFP with RBS 2008-10-27T12:00:00Z 2015-05-08T01:08:40Z registry plasmids Released HQ 2013 RFP, BBa_E1010, with Elowitz RBS, BBa_B0034. false false _247_ 0 3630 9 In stock false The part was needed for downstream applications. There can be no expression of a reporter without a ribosome binding site. true Kathy Lam, Danielle Nash component2244025 1 BBa_E1010 component2244022 1 BBa_B0034 annotation2244025 1 BBa_E1010 range2244025 1 19 724 annotation2244022 1 BBa_B0034 range2244022 1 1 12 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K196003 1 HfsH HfsH protein from <i>Caulobacter crescentus</i> 2009-08-11T11:00:00Z 2015-05-08T01:11:17Z This sequence comes from Caulobacter crescentus. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. Here you have HfsH. false false _309_ 0 4813 9 It's complicated false As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt. false Laetitia Warny BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_K196002 1 HfsG HfsG protein from <i>Caulobacter crescentus</i> 2009-08-10T11:00:00Z 2015-05-08T01:11:17Z This sequence comes from Caulobacter crescentus. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are differents proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we wanted this glue to be produced by Escherichia coli. As E. coli does have homologs for many of these proteins, but not for HfsG and HfsH, we decided to create a BB including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. false false _309_ 0 4813 9 It's complicated false As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt. false Laetitia Warny BBa_K196005 1 BBa_K196005 HfsG + HfsH proteins from <i>Caulobacter crescentus</i> + ter (BBa_B0015) 2009-08-12T11:00:00Z 2015-05-08T01:11:17Z HfsG and HfsH come from Caulobacter crescentus but were synthesized by Geneart to satisfy de standard 10. Intermediate part for the GluColi [http://2009.igem.org/Team:ULB-Brussels]. false true _309_ 0 4813 9 It's complicated false - false Laetitia Warny component2017276 1 BBa_B0012 component2017274 1 BBa_B0010 component2017272 1 BBa_K196002 component2017273 1 BBa_K196003 annotation2017272 1 BBa_K196002 range2017272 1 1 933 annotation2017274 1 BBa_B0010 range2017274 1 1725 1804 annotation2017273 1 BBa_K196003 range2017273 1 940 1716 annotation2017276 1 BBa_B0012 range2017276 1 1813 1853 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2001 1 lac O1 range2001 1 26 42 annotation2000 1 -35 range2000 1 20 25 annotation2002 1 -10 range2002 1 43 48 annotation1999 1 lac O1 range1999 1 3 19 annotation7064 1 BBa_R0011 range7064 1 1 54 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K093005_sequence 1 aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_B0034_sequence 1 aaagaggagaaa BBa_K196003_sequence 1 atgccgatggagttcgaaaaagtagatgcttatgaaccagaccgcagcctgaaaggcaaactgcgtcgccgtctgatccgtctggcacaccgccgtccagcaaaagtggctctggaacgtccgatggtgtctttctccttcgacgacgcgccagcaactgcttgcgaagctggcgcacgtgctctggaagctcgtggcctgcgtggcacctactatttcgctgctggtctgaccggccgtgacggccctatgggccgctatgcaactggtgaggacgcacgtcgtctgcacgaagccggtcacgaaatcgcttgccacacctactcccacctggattgtggtcagtcttctcagaccgaaaccctggctgatgtcgatcgtaatgccgaaagcctggcggcttggggtgcaggcgatccggtgtcttttgcctacccgtacggtgatgtggctgctccggctaaaacggctctgtctggtcgttttaaaactctgcgcgctctgcaccacggcctgatcaccgacggcgcagatctgaaccagactccggcagtaggcatcgaaggtgaagatggtgaaaccgttgccaaggcatggctggataaggcgaaggcacgtaaagcctggctgatcctgtatacgcacgacgtcgcaggccagcctagccagtggggttgcaccacggaagcgctggaacgcctgatcgaccgtgctctggcggacggcttcgacgttgtgacggtagccgaaggttctcgtcgtatcggcctgtaataa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K196014_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagatgaacgcccctgtaaatgaactgcgcctggagaatgctgcatgggcggcggcacagccgcgtctgtccgtgctgatccctaccttccgtgatgatccgtctgctctgctgaaggcgctggaccacaccaacgcagcggttgaagtcgtggtgctggacgatggtggcggcgacgatgctctggcggaacgcactgcgcgtcgcatcgagaaaatgcgtactccagcccgcttcgtacgtctgagccgtaacgaaggtcgtgccaaaggtcgtaaccgcctggcgagccatgctcgtggtggccacttcctgttcctggactctgacatgctgcctgacaccccggactttctggaccgttggtctgctgttgccgacaccggtgccgcggttgctttcggtggtttcaccctggaccagaccccgcagcgcccggaacatgctctgcatcgtgctatggcactgaaatctgactgcacgccggctccagaacgtgcgaaggccccggaaaaacacgttttcacgtccaacctgctggttcgtcgtgatgtattcgaaactgtgggttttgatgaaggtttctccggttggggttgggaagacgtcgaatgggccatgcgtgttgcgcgtcaacacccgatcctgcatatcgataacactgccacccacctgggcctggacccagcgccagtaatggcagccaaatatgaacaatctgccgcaaactttgcacgtgttgtcgcgagccaccgcgacgttgttagcgcatatccgtcttacaaggtagcgaaactgctgaaagcagtgccgctgatctccgtttggcgtccgctgctgaaacaggtcgcactggccgaagcggcaccggtaagcctgcgtgcattcgccatgcgtctgtaccgtgctgcgctgtacagcgaagctgtttaataatactagatgccgatggagttcgaaaaagtagatgcttatgaaccagaccgcagcctgaaaggcaaactgcgtcgccgtctgatccgtctggcacaccgccgtccagcaaaagtggctctggaacgtccgatggtgtctttctccttcgacgacgcgccagcaactgcttgcgaagctggcgcacgtgctctggaagctcgtggcctgcgtggcacctactatttcgctgctggtctgaccggccgtgacggccctatgggccgctatgcaactggtgaggacgcacgtcgtctgcacgaagccggtcacgaaatcgcttgccacacctactcccacctggattgtggtcagtcttctcagaccgaaaccctggctgatgtcgatcgtaatgccgaaagcctggcggcttggggtgcaggcgatccggtgtcttttgcctacccgtacggtgatgtggctgctccggctaaaacggctctgtctggtcgttttaaaactctgcgcgctctgcaccacggcctgatcaccgacggcgcagatctgaaccagactccggcagtaggcatcgaaggtgaagatggtgaaaccgttgccaaggcatggctggataaggcgaaggcacgtaaagcctggctgatcctgtatacgcacgacgtcgcaggccagcctagccagtggggttgcaccacggaagcgctggaacgcctgatcgaccgtgctctggcggacggcttcgacgttgtgacggtagccgaaggttctcgtcgtatcggcctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K196002_sequence 1 atgaacgcccctgtaaatgaactgcgcctggagaatgctgcatgggcggcggcacagccgcgtctgtccgtgctgatccctaccttccgtgatgatccgtctgctctgctgaaggcgctggaccacaccaacgcagcggttgaagtcgtggtgctggacgatggtggcggcgacgatgctctggcggaacgcactgcgcgtcgcatcgagaaaatgcgtactccagcccgcttcgtacgtctgagccgtaacgaaggtcgtgccaaaggtcgtaaccgcctggcgagccatgctcgtggtggccacttcctgttcctggactctgacatgctgcctgacaccccggactttctggaccgttggtctgctgttgccgacaccggtgccgcggttgctttcggtggtttcaccctggaccagaccccgcagcgcccggaacatgctctgcatcgtgctatggcactgaaatctgactgcacgccggctccagaacgtgcgaaggccccggaaaaacacgttttcacgtccaacctgctggttcgtcgtgatgtattcgaaactgtgggttttgatgaaggtttctccggttggggttgggaagacgtcgaatgggccatgcgtgttgcgcgtcaacacccgatcctgcatatcgataacactgccacccacctgggcctggacccagcgccagtaatggcagccaaatatgaacaatctgccgcaaactttgcacgtgttgtcgcgagccaccgcgacgttgttagcgcatatccgtcttacaaggtagcgaaactgctgaaagcagtgccgctgatctccgtttggcgtccgctgctgaaacaggtcgcactggccgaagcggcaccggtaagcctgcgtgcattcgccatgcgtctgtaccgtgctgcgctgtacagcgaagctgtttaataa BBa_K196007_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_K196005_sequence 1 atgaacgcccctgtaaatgaactgcgcctggagaatgctgcatgggcggcggcacagccgcgtctgtccgtgctgatccctaccttccgtgatgatccgtctgctctgctgaaggcgctggaccacaccaacgcagcggttgaagtcgtggtgctggacgatggtggcggcgacgatgctctggcggaacgcactgcgcgtcgcatcgagaaaatgcgtactccagcccgcttcgtacgtctgagccgtaacgaaggtcgtgccaaaggtcgtaaccgcctggcgagccatgctcgtggtggccacttcctgttcctggactctgacatgctgcctgacaccccggactttctggaccgttggtctgctgttgccgacaccggtgccgcggttgctttcggtggtttcaccctggaccagaccccgcagcgcccggaacatgctctgcatcgtgctatggcactgaaatctgactgcacgccggctccagaacgtgcgaaggccccggaaaaacacgttttcacgtccaacctgctggttcgtcgtgatgtattcgaaactgtgggttttgatgaaggtttctccggttggggttgggaagacgtcgaatgggccatgcgtgttgcgcgtcaacacccgatcctgcatatcgataacactgccacccacctgggcctggacccagcgccagtaatggcagccaaatatgaacaatctgccgcaaactttgcacgtgttgtcgcgagccaccgcgacgttgttagcgcatatccgtcttacaaggtagcgaaactgctgaaagcagtgccgctgatctccgtttggcgtccgctgctgaaacaggtcgcactggccgaagcggcaccggtaagcctgcgtgcattcgccatgcgtctgtaccgtgctgcgctgtacagcgaagctgtttaataatactagatgccgatggagttcgaaaaagtagatgcttatgaaccagaccgcagcctgaaaggcaaactgcgtcgccgtctgatccgtctggcacaccgccgtccagcaaaagtggctctggaacgtccgatggtgtctttctccttcgacgacgcgccagcaactgcttgcgaagctggcgcacgtgctctggaagctcgtggcctgcgtggcacctactatttcgctgctggtctgaccggccgtgacggccctatgggccgctatgcaactggtgaggacgcacgtcgtctgcacgaagccggtcacgaaatcgcttgccacacctactcccacctggattgtggtcagtcttctcagaccgaaaccctggctgatgtcgatcgtaatgccgaaagcctggcggcttggggtgcaggcgatccggtgtcttttgcctacccgtacggtgatgtggctgctccggctaaaacggctctgtctggtcgttttaaaactctgcgcgctctgcaccacggcctgatcaccgacggcgcagatctgaaccagactccggcagtaggcatcgaaggtgaagatggtgaaaccgttgccaaggcatggctggataaggcgaaggcacgtaaagcctggctgatcctgtatacgcacgacgtcgcaggccagcctagccagtggggttgcaccacggaagcgctggaacgcctgatcgaccgtgctctggcggacggcttcgacgttgtgacggtagccgaaggttctcgtcgtatcggcctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z