GluColi
type: ComponentDefinition
persistent identity: BBa K196014
display id: BBa_K196014
version: 1
was generated by: iGEM to SBOL conversion
description: Final GluColi device.
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:17Z
mutable provenance: HfsG and HfsH sequences come from Caulobacter crescentus.
owned by: james
owned by: myers
mutable description: This is the part we actually created to test the glue production by E. coli. HfsG and HfsH come from Caulobacter crescentu. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see our wiki page [1]. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. RFP has been added as a reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _309_
m user id: 0
owner id: 4813
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: As many mutations were needed to make the part compatible with the standard 10, we decided to make hfsG and hfsH synthetized by GeneArt [2]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.
star: false
creator: Laetitia Warny
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K196005
component: BBa_K196007
sequence annotation: BBa_K196007
sequence annotation: BBa_K196005
sequence: https://synbiohub.org/public/igem/BBa_K196014_sequence/1

BBa_K196005
type: Component
persistent identity: component2267888
display id: component2267888
version: 1
top level: GluColi
definition: BBa_K196005
access: public

BBa_K196007
type: Component
persistent identity: component2267879
display id: component2267879
version: 1
top level: GluColi
definition: BBa_K196007
access: public

BBa_K196007
type: SequenceAnnotation
persistent identity: annotation2267879
display id: annotation2267879
version: 1
top level: GluColi
location: https://synbiohub.org/public/igem/BBa_K196014/annotation2267879/range2267879/1
component: BBa_K196007

https://synbiohub.org/public/igem/BBa_K196014/annotation2267879/range2267879/1
type: Range
persistent identity: range2267879
display id: range2267879
version: 1
top level: GluColi
direction: direction/reverse
start: 1
end: 787
orientation: inline

BBa_K196005
type: SequenceAnnotation
persistent identity: annotation2267888
display id: annotation2267888
version: 1
top level: GluColi
location: https://synbiohub.org/public/igem/BBa_K196014/annotation2267888/range2267888/1
component: BBa_K196005

https://synbiohub.org/public/igem/BBa_K196014/annotation2267888/range2267888/1
type: Range
persistent identity: range2267888
display id: range2267888
version: 1
top level: GluColi
direction: direction/reverse
start: 794
end: 2646
orientation: inline

BBa_K196005
type: ComponentDefinition
persistent identity: BBa K196005
display id: BBa_K196005
version: 1
was generated by: iGEM to SBOL conversion
description: HfsG + HfsH proteins from Caulobacter crescentus + ter (BBa_B0015)
created: 2009-08-12T11:00:00Z
modified: 2015-05-08T01:11:17Z
mutable provenance: HfsG and HfsH come from Caulobacter crescentus but were synthesized by Geneart to satisfy de standard 10.
owned by: james
owned by: myers
mutable description: Intermediate part for the GluColi [http://2009.igem.org/Team:ULB-Brussels].
discontinued: false
dominant: true
experience: experience/None
group u list: _309_
m user id: 0
owner id: 4813
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Laetitia Warny
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K196002
component: BBa_B0010
component: BBa_K196003
component: BBa_B0012
sequence annotation: BBa_K196002
sequence annotation: BBa_K196003
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K196005_sequence/1

BBa_K196002
type: Component
persistent identity: component2017272
display id: component2017272
version: 1
top level: BBa_K196005
definition: HfsG
access: public

BBa_B0010
type: Component
persistent identity: component2017274
display id: component2017274
version: 1
top level: BBa_K196005
definition: BBa_B0010
access: public

BBa_K196003
type: Component
persistent identity: component2017273
display id: component2017273
version: 1
top level: BBa_K196005
definition: HfsH
access: public

BBa_B0012
type: Component
persistent identity: component2017276
display id: component2017276
version: 1
top level: BBa_K196005
definition: BBa_B0012
access: public

BBa_K196002
type: SequenceAnnotation
persistent identity: annotation2017272
display id: annotation2017272
version: 1
top level: BBa_K196005
location: https://synbiohub.org/public/igem/BBa_K196005/annotation2017272/range2017272/1
component: BBa_K196002

https://synbiohub.org/public/igem/BBa_K196005/annotation2017272/range2017272/1
type: Range
persistent identity: range2017272
display id: range2017272
version: 1
top level: BBa_K196005
direction: direction/reverse
start: 1
end: 933
orientation: inline

BBa_K196003
type: SequenceAnnotation
persistent identity: annotation2017273
display id: annotation2017273
version: 1
top level: BBa_K196005
location: https://synbiohub.org/public/igem/BBa_K196005/annotation2017273/range2017273/1
component: BBa_K196003

https://synbiohub.org/public/igem/BBa_K196005/annotation2017273/range2017273/1
type: Range
persistent identity: range2017273
display id: range2017273
version: 1
top level: BBa_K196005
direction: direction/reverse
start: 940
end: 1716
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2017274
display id: annotation2017274
version: 1
top level: BBa_K196005
location: https://synbiohub.org/public/igem/BBa_K196005/annotation2017274/range2017274/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K196005/annotation2017274/range2017274/1
type: Range
persistent identity: range2017274
display id: range2017274
version: 1
top level: BBa_K196005
direction: direction/forward
start: 1725
end: 1804
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2017276
display id: annotation2017276
version: 1
top level: BBa_K196005
location: https://synbiohub.org/public/igem/BBa_K196005/annotation2017276/range2017276/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K196005/annotation2017276/range2017276/1
type: Range
persistent identity: range2017276
display id: range2017276
version: 1
top level: BBa_K196005
direction: direction/forward
start: 1813
end: 1853
orientation: inline

BBa_K196007
type: ComponentDefinition
persistent identity: BBa K196007
display id: BBa_K196007
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid) + RFP + RBS (GluColi project)
created: 2009-08-12T11:00:00Z
modified: 2015-05-08T01:11:17Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: Intermediate in the GluColi project in order to have a construction with a reporter.
discontinued: false
dominant: false
experience: experience/None
group u list: _309_
m user id: 0
owner id: 4813
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Laetitia Warny
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0011
component: BBa_K093005
sequence annotation: BBa_R0011
sequence annotation: BBa_K093005
sequence: https://synbiohub.org/public/igem/BBa_K196007_sequence/1

BBa_R0011
type: Component
persistent identity: component2246500
display id: component2246500
version: 1
top level: BBa_K196007
definition: lacI+pL
access: public

BBa_K093005
type: Component
persistent identity: component2246510
display id: component2246510
version: 1
top level: BBa_K196007
definition: RBS-RFP
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2246500
display id: annotation2246500
version: 1
top level: BBa_K196007
location: https://synbiohub.org/public/igem/BBa_K196007/annotation2246500/range2246500/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K196007/annotation2246500/range2246500/1
type: Range
persistent identity: range2246500
display id: range2246500
version: 1
top level: BBa_K196007
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K093005
type: SequenceAnnotation
persistent identity: annotation2246510
display id: annotation2246510
version: 1
top level: BBa_K196007
location: https://synbiohub.org/public/igem/BBa_K196007/annotation2246510/range2246510/1
component: BBa_K093005

https://synbiohub.org/public/igem/BBa_K196007/annotation2246510/range2246510/1
type: Range
persistent identity: range2246510
display id: range2246510
version: 1
top level: BBa_K196007
direction: direction/reverse
start: 64
end: 787
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

RBS-RFP
type: ComponentDefinition
persistent identity: BBa K093005
display id: BBa_K093005
version: 1
was generated by: iGEM to SBOL conversion
description: RFP with RBS
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: registry plasmids
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RFP, BBa_E1010, with Elowitz RBS, BBa_B0034.
discontinued: false
dominant: false
experience: experience/Works
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The part was needed for downstream applications. There can be no expression of a reporter without a ribosome binding site.
star: true
creator: Kathy Lam, Danielle Nash
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K093005_sequence/1

BBa_B0034
type: Component
persistent identity: component2244022
display id: component2244022
version: 1
top level: RBS-RFP
definition: BBa_B0034
access: public

BBa_E1010
type: Component
persistent identity: component2244025
display id: component2244025
version: 1
top level: RBS-RFP
definition: mRFP1
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2244022
display id: annotation2244022
version: 1
top level: RBS-RFP
location: https://synbiohub.org/public/igem/BBa_K093005/annotation2244022/range2244022/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K093005/annotation2244022/range2244022/1
type: Range
persistent identity: range2244022
display id: range2244022
version: 1
top level: RBS-RFP
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2244025
display id: annotation2244025
version: 1
top level: RBS-RFP
location: https://synbiohub.org/public/igem/BBa_K093005/annotation2244025/range2244025/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K093005/annotation2244025/range2244025/1
type: Range
persistent identity: range2244025
display id: range2244025
version: 1
top level: RBS-RFP
direction: direction/reverse
start: 19
end: 724
orientation: inline

HfsG
type: ComponentDefinition
persistent identity: BBa K196002
display id: BBa_K196002
version: 1
was generated by: iGEM to SBOL conversion
description: HfsG protein from Caulobacter crescentus
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:11:17Z
mutable provenance: This sequence comes from Caulobacter crescentus.
owned by: james
owned by: myers
mutable description: Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are differents proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we wanted this glue to be produced by Escherichia coli. As E. coli does have homologs for many of these proteins, but not for HfsG and HfsH, we decided to create a BB including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase.
discontinued: false
dominant: false
experience: experience/None
group u list: _309_
m user id: 0
owner id: 4813
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt.
star: false
creator: Laetitia Warny
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K196002_sequence/1

HfsH
type: ComponentDefinition
persistent identity: BBa K196003
display id: BBa_K196003
version: 1
was generated by: iGEM to SBOL conversion
description: HfsH protein from Caulobacter crescentus
created: 2009-08-11T11:00:00Z
modified: 2015-05-08T01:11:17Z
mutable provenance: This sequence comes from Caulobacter crescentus.
owned by: james
owned by: myers
mutable description: Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. Here you have HfsH.
discontinued: false
dominant: false
experience: experience/None
group u list: _309_
m user id: 0
owner id: 4813
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt.
star: false
creator: Laetitia Warny
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K196003_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

https://synbiohub.org/public/igem/BBa_K196014_sequence/1
type: Sequence
persistent identity: BBa K196014 sequence
display id: BBa_K196014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagatgaacgcccctgtaaatgaactgcgcctggagaatgctgcatgggcggcggcacagccgcgtctgtccgtgctgatccctaccttccgtgatgatccgtctgctctgctgaaggcgctggaccacaccaacgcagcggttgaagtcgtggtgctggacgatggtggcggcgacgatgctctggcggaacgcactgcgcgtcgcatcgagaaaatgcgtactccagcccgcttcgtacgtctgagccgtaacgaaggtcgtgccaaaggtcgtaaccgcctggcgagccatgctcgtggtggccacttcctgttcctggactctgacatgctgcctgacaccccggactttctggaccgttggtctgctgttgccgacaccggtgccgcggttgctttcggtggtttcaccctggaccagaccccgcagcgcccggaacatgctctgcatcgtgctatggcactgaaatctgactgcacgccggctccagaacgtgcgaaggccccggaaaaacacgttttcacgtccaacctgctggttcgtcgtgatgtattcgaaactgtgggttttgatgaaggtttctccggttggggttgggaagacgtcgaatgggccatgcgtgttgcgcgtcaacacccgatcctgcatatcgataacactgccacccacctgggcctggacccagcgccagtaatggcagccaaatatgaacaatctgccgcaaactttgcacgtgttgtcgcgagccaccgcgacgttgttagcgcatatccgtcttacaaggtagcgaaactgctgaaagcagtgccgctgatctccgtttggcgtccgctgctgaaacaggtcgcactggccgaagcggcaccggtaagcctgcgtgcattcgccatgcgtctgtaccgtgctgcgctgtacagcgaagctgtttaataatactagatgccgatggagttcgaaaaagtagatgcttatgaaccagaccgcagcctgaaaggcaaactgcgtcgccgtctgatccgtctggcacaccgccgtccagcaaaagtggctctggaacgtccgatggtgtctttctccttcgacgacgcgccagcaactgcttgcgaagctggcgcacgtgctctggaagctcgtggcctgcgtggcacctactatttcgctgctggtctgaccggccgtgacggccctatgggccgctatgcaactg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K196005_sequence/1
type: Sequence
persistent identity: BBa K196005 sequence
display id: BBa_K196005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacgcccctgtaaatgaactgcgcctggagaatgctgcatgggcggcggcacagccgcgtctgtccgtgctgatccctaccttccgtgatgatccgtctgctctgctgaaggcgctggaccacaccaacgcagcggttgaagtcgtggtgctggacgatggtggcggcgacgatgctctggcggaacgcactgcgcgtcgcatcgagaaaatgcgtactccagcccgcttcgtacgtctgagccgtaacgaaggtcgtgccaaaggtcgtaaccgcctggcgagccatgctcgtggtggccacttcctgttcctggactctgacatgctgcctgacaccccggactttctggaccgttggtctgctgttgccgacaccggtgccgcggttgctttcggtggtttcaccctggaccagaccccgcagcgcccggaacatgctctgcatcgtgctatggcactgaaatctgactgcacgccggctccagaacgtgcgaaggccccggaaaaacacgttttcacgtccaacctgctggttcgtcgtgatgtattcgaaactgtgggttttgatgaaggtttctccggttggggttgggaagacgtcgaatgggccatgcgtgttgcgcgtcaacacccgatcctgcatatcgataacactgccacccacctgggcctggacccagcgccagtaatggcagccaaatatgaacaatctgccgcaaactttgcacgtgttgtcgcgagccaccgcgacgttgttagcgcatatccgtcttacaaggtagcgaaactgctgaaagcagtgccgctgatctccgtttggcgtccgctgctgaaacaggtcgcactggccgaagcggcaccggtaagcctgcgtgcattcgccatgcgtctgtaccgtgctgcgctgtacagcgaagctgtttaataatactagatgccgatggagttcgaaaaagtagatgcttatgaaccagaccgcagcctgaaaggcaaactgcgtcgccgtctgatccgtctggcacaccgccgtccagcaaaagtggctctggaacgtccgatggtgtctttctccttcgacgacgcgccagcaactgcttgcgaagctggcgcacgtgctctggaagctcgtggcctgcgtggcacctactatttcgctgctggtctgaccggccgtgacggccctatgggccgctatgcaactggtgaggacgcacgtcgtctgcacgaagccggtcacgaaatcgcttgccacacctactcccacctggattgtggtcagtcttctcagaccgaaaccctggctgatgtcgatcgtaatgccgaaagcctggcggcttggggtgcaggcgatccggtgtcttttgcctacccgtacggtgatgtggctgctccggctaaaacggctctgtctggtcgttttaaaactctgcgcgctctgcaccacggcctgatcaccgacggcgcagatctgaaccagactccggcagtaggcatcgaaggtgaagatggtgaaaccgttgccaaggcatggctggataaggcgaaggcacgtaaagcctggctgatcctgtatacgcacgacgtcgcaggccagcctagccagtggggttgcaccacggaagcgctggaacgcctgatcgaccgtgctctggcggacggcttcgacgttgtgacggtagccgaaggttctcgtcgtatcggcctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K196007_sequence/1
type: Sequence
persistent identity: BBa K196007 sequence
display id: BBa_K196007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K093005_sequence/1
type: Sequence
persistent identity: BBa K093005 sequence
display id: BBa_K093005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K196002_sequence/1
type: Sequence
persistent identity: BBa K196002 sequence
display id: BBa_K196002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacgcccctgtaaatgaactgcgcctggagaatgctgcatgggcggcggcacagccgcgtctgtccgtgctgatccctaccttccgtgatgatccgtctgctctgctgaaggcgctggaccacaccaacgcagcggttgaagtcgtggtgctggacgatggtggcggcgacgatgctctggcggaacgcactgcgcgtcgcatcgagaaaatgcgtactccagcccgcttcgtacgtctgagccgtaacgaaggtcgtgccaaaggtcgtaaccgcctggcgagccatgctcgtggtggccacttcctgttcctggactctgacatgctgcctgacaccccggactttctggaccgttggtctgctgttgccgacaccggtgccgcggttgctttcggtggtttcaccctggaccagaccccgcagcgcccggaacatgctctgcatcgtgctatggcactgaaatctgactgcacgccggctccagaacgtgcgaaggccccggaaaaacacgttttcacgtccaacctgctggttcgtcgtgatgtattcgaaactgtgggttttgatgaaggtttctccggttggggttgggaagacgtcgaatgggccatgcgtgttgcgcgtcaacacccgatcctgcatatcgataacactgccacccacctgggcctggacccagcgccagtaatggcagccaaatatgaacaatctgccgcaaactttgcacgtgttgtcgcgagccaccgcgacgttgttagcgcatatccgtcttacaaggtagcgaaactgctgaaagcagtgccgctgatctccgtttggcgtccgctgctgaaacaggtcgcactggccgaagcggcaccggtaagcctgcgtgcattcgccatgcgtctgtaccgtgctgcgctgtacagcgaagctgtttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K196003_sequence/1
type: Sequence
persistent identity: BBa K196003 sequence
display id: BBa_K196003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccgatggagttcgaaaaagtagatgcttatgaaccagaccgcagcctgaaaggcaaactgcgtcgccgtctgatccgtctggcacaccgccgtccagcaaaagtggctctggaacgtccgatggtgtctttctccttcgacgacgcgccagcaactgcttgcgaagctggcgcacgtgctctggaagctcgtggcctgcgtggcacctactatttcgctgctggtctgaccggccgtgacggccctatgggccgctatgcaactggtgaggacgcacgtcgtctgcacgaagccggtcacgaaatcgcttgccacacctactcccacctggattgtggtcagtcttctcagaccgaaaccctggctgatgtcgatcgtaatgccgaaagcctggcggcttggggtgcaggcgatccggtgtcttttgcctacccgtacggtgatgtggctgctccggctaaaacggctctgtctggtcgttttaaaactctgcgcgctctgcaccacggcctgatcaccgacggcgcagatctgaaccagactccggcagtaggcatcgaaggtgaagatggtgaaaccgttgccaaggcatggctggataaggcgaaggcacgtaaagcctggctgatcctgtatacgcacgacgtcgcaggccagcctagccagtggggttgcaccacggaagcgctggaacgcctgatcgaccgtgctctggcggacggcttcgacgttgtgacggtagccgaaggttctcgtcgtatcggcctgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z