LOCUS       BBa_K1961004            2253 bp    DNA     linear       22-Nov-2024
DEFINITION  PbrR generator expressed by GFP under autoregulation of PTet
ACCESSION   BBa_K1961004
VERSION     BBa_K1961004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             84..700
                     /label=tetR
     misc_feature    701..734
                     /label=SsrA
     misc_feature    741..765
                     /label=Help:Barcodes
     CDS             81..765
                     /label=BBa_C0040
     stem_loop       785..828
                     /label=stem_loop
     misc_feature    774..853
                     /label=BBa_B0010
     terminator      774..853
                     /label=BBa_B0010
     stem_loop       869..888
                     /label=T7 TE
     misc_feature    895..895
                     /label=stop
     polyA_site      889..902
                     /label=polya
     misc_feature    862..902
                     /label=BBa_B0012
     terminator      862..902
                     /label=BBa_B0012
     misc_feature    911..929
                     /label=TetR 1
     misc_feature    911..964
                     /label=BBa_R0040
     promoter        930..935
                     /label=-35
     misc_feature    936..954
                     /label=TetR 2
     promoter        953..958
                     /label=-10
     promoter        911..964
                     /label=BBa_R0040
     RBS             973..985
                     /label=RBS-3\Weak
     RBS             979..982
                     /label=RBS
     misc_feature    973..985
                     /label=BBa_B0032
     RBS             973..985
                     /label=BBa_B0032
     CDS             992..1390
                     /label=BBa_I721002
     CDS             1397..2116
                     /label=GFP protein
     CDS             1397..2116
                     /label=BBa_E0040
     stem_loop       2136..2179
                     /label=stem_loop
     misc_feature    2125..2204
                     /label=BBa_B0010
     terminator      2125..2204
                     /label=BBa_B0010
     stem_loop       2220..2239
                     /label=T7 TE
     misc_feature    2246..2246
                     /label=stop
     polyA_site      2240..2253
                     /label=polya
     misc_feature    2213..2253
                     /label=BBa_B0012
     terminator      2213..2253
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat
      121 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      181 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      241 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      301 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      361 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      421 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      481 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      541 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      601 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      661 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      721 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca
      781 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      841 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      901 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
      961 gcactactag agtcacacag gaaagtacta gatgatgcgg atcggtgaac tgggcaagaa
     1021 ggcagattgc ttggtgcaga ccgtgcgctt ttacgagtca gaaggcttgc tgcccgagcc
     1081 tgcacgtagc gagggcaact tcaggctcta tgacgaagtc catttgcagc gcttgctgtt
     1141 catccgccgc tgccgggcga aggacatgac gctggatgag atccgtcaac tgctgaactt
     1201 acgggatcgg ccagagttgg gctgcggcga ggtgaacgcg ctggtcgacg ctcatatcgc
     1261 gcaagtgcgg accaagatga aggaattgcg cgccttggag cgcgagttaa tggatctgcg
     1321 acgctcctgc gatagcgccc gaacctcgcg cgagtgcggc attctcaaca gcttggccga
     1381 gcccgcctga tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
     1441 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
     1501 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
     1561 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
     1621 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     1681 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
     1741 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     1801 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     1861 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     1921 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     1981 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     2041 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     2101 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     2161 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     2221 gctcaccttc gggtgggcct ttctgcgttt ata
//