##gff-version 3
##sequence-region BBa_K1961004 1 2253
BBa_K1961004	.	ribosome_entry_site	973	985	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K1961004	.	ribosome_entry_site	979	982	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1961004	.	ribosome_entry_site	973	985	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1961004	.	engineered_region	973	985	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1961004	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1961004	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1961004	.	CDS	1397	2116	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K1961004	.	CDS	1397	2116	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1961004	.	terminator	774	853	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1961004	.	engineered_region	774	853	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1961004	.	stem_loop	785	828	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1961004	.	promoter	911	964	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1961004	.	promoter	930	935	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1961004	.	non_covalent_binding_site	936	954	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1961004	.	engineered_region	911	964	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1961004	.	promoter	953	958	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1961004	.	non_covalent_binding_site	911	929	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1961004	.	terminator	2213	2253	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1961004	.	engineered_region	2213	2253	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1961004	.	stop_codon	2246	2246	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1961004	.	stem_loop	2220	2239	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1961004	.	polyA_site	2240	2253	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1961004	.	terminator	2125	2204	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1961004	.	engineered_region	2125	2204	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1961004	.	stem_loop	2136	2179	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1961004	.	terminator	862	902	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1961004	.	engineered_region	862	902	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1961004	.	stop_codon	895	895	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1961004	.	stem_loop	869	888	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1961004	.	polyA_site	889	902	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1961004	.	CDS	81	765	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K1961004	.	engineered_tag	741	765	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1961004	.	tag	701	734	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1961004	.	CDS	84	700	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1961004	.	CDS	992	1390	.	+	0	ID=BBa_I721002;Name=BBa_I721002
BBa_K1961004	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1961004	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1961004	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1961004	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1961004	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1961004	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K1961004
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtga
tagagatactgagcactactagagtcacacaggaaagtactagatgatgcggatcggtgaactgggcaagaaggcagat
tgcttggtgcagaccgtgcgcttttacgagtcagaaggcttgctgcccgagcctgcacgtagcgagggcaacttcaggc
tctatgacgaagtccatttgcagcgcttgctgttcatccgccgctgccgggcgaaggacatgacgctggatgagatccg
tcaactgctgaacttacgggatcggccagagttgggctgcggcgaggtgaacgcgctggtcgacgctcatatcgcgcaa
gtgcggaccaagatgaaggaattgcgcgccttggagcgcgagttaatggatctgcgacgctcctgcgatagcgcccgaa
cctcgcgcgagtgcggcattctcaacagcttggccgagcccgcctgatactagatgcgtaaaggagaagaacttttcac
tggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggt
gatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtca
ctactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccat
gcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagttt
gaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaat
tggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaat
tagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtc
cttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcc
ttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata