LOCUS       BBa_K1961005            1324 bp    DNA     linear       26-Nov-2024
DEFINITION  PbrR generator expressed by GFP under control of general promoter 
            (J23102)
ACCESSION   BBa_K1961005
VERSION     BBa_K1961005.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..461
                     /label=BBa_I721002
     CDS             468..1187
                     /label=GFP protein
     CDS             468..1187
                     /label=BBa_E0040
     stem_loop       1207..1250
                     /label=stem_loop
     misc_feature    1196..1275
                     /label=BBa_B0010
     terminator      1196..1275
                     /label=BBa_B0010
     stem_loop       1291..1310
                     /label=T7 TE
     misc_feature    1317..1317
                     /label=stop
     polyA_site      1311..1324
                     /label=polya
     misc_feature    1284..1324
                     /label=BBa_B0012
     terminator      1284..1324
                     /label=BBa_B0012
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagtcacaca ggaaagtact
       61 agatgatgcg gatcggtgaa ctgggcaaga aggcagattg cttggtgcag accgtgcgct
      121 tttacgagtc agaaggcttg ctgcccgagc ctgcacgtag cgagggcaac ttcaggctct
      181 atgacgaagt ccatttgcag cgcttgctgt tcatccgccg ctgccgggcg aaggacatga
      241 cgctggatga gatccgtcaa ctgctgaact tacgggatcg gccagagttg ggctgcggcg
      301 aggtgaacgc gctggtcgac gctcatatcg cgcaagtgcg gaccaagatg aaggaattgc
      361 gcgccttgga gcgcgagtta atggatctgc gacgctcctg cgatagcgcc cgaacctcgc
      421 gcgagtgcgg cattctcaac agcttggccg agcccgcctg atactagatg cgtaaaggag
      481 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      541 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      601 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
      661 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
      721 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      781 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      841 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      901 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      961 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     1021 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     1081 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     1141 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagccagg
     1201 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1261 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1321 tata
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