##gff-version 3
##sequence-region BBa_K1961006 1 1569
BBa_K1961006	.	terminator	558	598	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1961006	.	polyA_site	585	598	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1961006	.	engineered_region	558	598	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1961006	.	stop_codon	591	591	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1961006	.	stem_loop	565	584	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1961006	.	terminator	470	549	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1961006	.	engineered_region	470	549	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1961006	.	stem_loop	481	524	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1961006	.	CDS	727	1432	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K1961006	.	engineered_tag	1408	1432	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1961006	.	CDS	727	1401	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1961006	.	terminator	1441	1520	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1961006	.	engineered_region	1441	1520	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1961006	.	stem_loop	1452	1495	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1961006	.	ribosome_entry_site	44	56	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K1961006	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1961006	.	engineered_region	44	56	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1961006	.	ribosome_entry_site	44	56	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1961006	.	promoter	1	35	.	+	0	ID=BBa_J23102;Name=BBa_J23102
BBa_K1961006	.	ribosome_entry_site	709	720	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1961006	.	conserved_region	713	716	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1961006	.	CDS	63	461	.	+	0	ID=BBa_I721002;Name=BBa_I721002
BBa_K1961006	.	promoter	607	700	.	+	0	ID=BBa_I721001;Name=BBa_I721001
BBa_K1961006	.	terminator	1529	1569	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1961006	.	polyA_site	1556	1569	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1961006	.	engineered_region	1529	1569	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1961006	.	stop_codon	1562	1562	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1961006	.	stem_loop	1536	1555	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_K1961006
ttgacagctagctcagtcctaggtactgtgctagctactagagtcacacaggaaagtactagatgatgcggatcggtga
actgggcaagaaggcagattgcttggtgcagaccgtgcgcttttacgagtcagaaggcttgctgcccgagcctgcacgt
agcgagggcaacttcaggctctatgacgaagtccatttgcagcgcttgctgttcatccgccgctgccgggcgaaggaca
tgacgctggatgagatccgtcaactgctgaacttacgggatcggccagagttgggctgcggcgaggtgaacgcgctggt
cgacgctcatatcgcgcaagtgcggaccaagatgaaggaattgcgcgccttggagcgcgagttaatggatctgcgacgc
tcctgcgatagcgcccgaacctcgcgcgagtgcggcattctcaacagcttggccgagcccgcctgatactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagggttgcttcctataaaaaacttgact
ctatatctactagaggttttctaatgatggcatccggggaaaaccttgtcaatgaagagcgatctatgtactagagaaa
gaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccg
ttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagt
taccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaa
cacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaag
acggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtac
caacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaa
gacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaacca
cctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacga
agactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctag
tgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata