BBa_K1961001
1
BBa_K1961001
RecF protein-SOS mediator protein
2016-10-02T11:00:00Z
2016-10-20T09:52:45Z
NCBI, GeneBank:CP016404.1
The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. When DNA is damaged and forms single-stranded DNA, Rec A binds single-stranded DNA (ssDNA). The binding of RecA onto ssDNA forms a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response). Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA.
false
false
_2428_
32606
32606
9
false
No
false
Yi-Ting Lin
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K081005
1
BBa_K081005
constitutive promoter family member and RBS
2008-10-17T11:00:00Z
2015-05-08T01:08:34Z
Promoter: John Anderson.
RBS: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Released HQ 2013
Constitutive promoter (strong) with RBS (strong, efficiency=0.3)
false
true
_227_
0
2583
9
In stock
true
We used BioBrick Standard Assembly.
true
Lorenzo Pasotti, Paolo Magni
component1981863
1
BBa_J23100
component1981865
1
BBa_B0030
annotation1981865
1
BBa_B0030
range1981865
1
44
58
annotation1981863
1
BBa_J23100
range1981863
1
1
35
BBa_K1961008
1
BBa_K1961008
RecF protein generator
2016-10-13T11:00:00Z
2016-10-14T10:43:17Z
We ligased K1961001 with the existing part of BBa_K081005
RecF protein-SOS mediator protein. It is involved in the RecFOR pathway for DNA metabolism; it is required for DNA replication and normal SOS inducibility
false
false
_2428_
32606
32606
9
false
No
false
Yi-Ting Lin
component2506937
1
BBa_K081005
component2506938
1
BBa_K1961001
annotation2506938
1
BBa_K1961001
range2506938
1
65
1138
annotation2506937
1
BBa_K081005
range2506937
1
1
58
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K1961008_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgtccctcacccgcttgttgatccgcgatttccgcaacattgaaaccgcggatctcgccttatctcccggctttaactttctggtaggtgccaacggcagtggcaaaaccagcgtgctggaagccatctatacgctcggccatggtcgggcgtttcgcagtttgcagattggtcgcgtcattcgccatgagcaggaggcgtttgttctccacgggcgattacagggcgaagagcgcgagacagcgattggcttaaccaaagacaaacagggcgacagcaaagtccgcatcgacggtacagacgggcataaggtcgcggaactggcgcacctgatgccaatgcagttgataacgccagaagggtttactttactcaacggcggccccaaatacagaagagcattcctcgactggggatgctttcacaacgaacccggatttttcaccgcctggagcaatctcaagcgattgctcaagcagcgcaatgcggcgctgcgccaggtgacacgttacgaacagctacgcccgtgggataaagagctgatcccgctggcggagcaaatcagcacctggcgcgcggagtatagcgccggtatcgcggccgatatggctgatacctgtaagcaatttctccctgagttttctctgactttctctttccagcgcggctgggagaaagagacagaatatgctgaggtgctggaacgtaattttgaacgcgatcgccagctaacctacaccgcgcacggcccgcacaaagcggacttacgcattcgcgccgacggtgcgccggtggaagataccttatcgcgtgggcagcttaagctgttgatgtgcgccttacgtctggcgcaaggagagttcctcacccgtgaaagcgggcggcggtgtctctacctgatagatgattttgcctctgagcttgatgatgagcgtcgcgggctgcttgccagccgcttaaaagcgacgcaatcacaggtctttgtcagcgcgatcagtgctgaacacgttatagacatgtcggacgaaaattcgaagatgtttaccgtggaaaagggtaaaataacggattaa
BBa_K081005_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1961001_sequence
1
atgtccctcacccgcttgttgatccgcgatttccgcaacattgaaaccgcggatctcgccttatctcccggctttaactttctggtaggtgccaacggcagtggcaaaaccagcgtgctggaagccatctatacgctcggccatggtcgggcgtttcgcagtttgcagattggtcgcgtcattcgccatgagcaggaggcgtttgttctccacgggcgattacagggcgaagagcgcgagacagcgattggcttaaccaaagacaaacagggcgacagcaaagtccgcatcgacggtacagacgggcataaggtcgcggaactggcgcacctgatgccaatgcagttgataacgccagaagggtttactttactcaacggcggccccaaatacagaagagcattcctcgactggggatgctttcacaacgaacccggatttttcaccgcctggagcaatctcaagcgattgctcaagcagcgcaatgcggcgctgcgccaggtgacacgttacgaacagctacgcccgtgggataaagagctgatcccgctggcggagcaaatcagcacctggcgcgcggagtatagcgccggtatcgcggccgatatggctgatacctgtaagcaatttctccctgagttttctctgactttctctttccagcgcggctgggagaaagagacagaatatgctgaggtgctggaacgtaattttgaacgcgatcgccagctaacctacaccgcgcacggcccgcacaaagcggacttacgcattcgcgccgacggtgcgccggtggaagataccttatcgcgtgggcagcttaagctgttgatgtgcgccttacgtctggcgcaaggagagttcctcacccgtgaaagcgggcggcggtgtctctacctgatagatgattttgcctctgagcttgatgatgagcgtcgcgggctgcttgccagccgcttaaaagcgacgcaatcacaggtctttgtcagcgcgatcagtgctgaacacgttatagacatgtcggacgaaaattcgaagatgtttaccgtggaaaagggtaaaataacggattaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z