BBa_K1962000 1 BBa_K1962000 Colicin Ia 2016-10-10T11:00:00Z 2016-10-11T02:23:41Z Optimised DNA sequence based on back-translation of the protein sequence was designed by Dundee iGEM (2016) and synthesised synthetically by IDT. This sequence codes for the full length Colicin Ia bacteriocin (anti-bacterial toxin). Colicin Ia is a protein that is used by species of E. coli in order to kill closely related species of E. coli in certain circumstances e.g. competition. The producer cell normally contains a protein which confers immunity to this toxin, which in this case is (BBA_). And this protein is believed to form a complex with the cytotoxic domain of the bacterocin rendering it inactive. When this bacteriocin is secreted the immunity protein dissociates from the complex leaving the cytotoxic domain active resulting in the pore forming activity of this bacteriocin returning and having effect on the target cells. The pore forming activity of this bacteriocin results in the depolarisation of the bacterial inner membrane resulting in the collapse of the protonmotive force. false false _2429_ 8083 8083 9 false Confers to REF[10]. false Frank Sargent annotation2491782 1 Colicin Ia range2491782 1 1 1881 annotation2491783 1 Bacteriocin (Toxin) Domain range2491783 1 1351 1881 BBa_K1962000_sequence 1 atgatgtccgatccggtccgtattacaaatcctggtgcagagtcccttggatacgactctgacgggcacgagattatggcagtagacatctatgtgaaccctccgcgtgtggacgttttccacggaaccccgccggcgtggtcttctttcggtaacaaaaccatctggggcggtaatgaatgggtagatgatagtcctacgcgctcagatatcgaaaagcgtgacaaggaaattacggcgtacaaaaacactttgtctgctcagcaaaaggaaaacgaaaacaaacgtaccgaagcgggtaaacgtctgtctgcggcgatcgcggcgcgtgaaaaggacgagaacacattgaagactcttcgcgcgggtaatgcagacgcggcggatatcactcgtcaagagttccgtctgttacaagcggaactgcgtgagtatggatttcgtaccgaaatcgcgggttacgacgcgctgcgtcttcatactgaaagccgcatgttatttgcagatgcagatagcttgcgtatctctccgcgtgaagcgcgttctctgatcgaacaggccgaaaaacgtcagaaagacgcgcagaacgcggacaaaaaagcagccgatatgctggccgaatatgagcgtcgcaaggggattcttgatacccgccttagcgagctggagaaaaacggcggggcagcacttgctgttttggacgcgcaacaggcgcgtctgttgggacagcaaacgcgcaacgaccgtgctatttctgaagcgcgtaacaaactgtcttctgttaccgaatctctgaacaccgcgcgcaacgcccttacgcgtgccgaacagcaactgacacaacagaagaatactccagacggtaaaaccatcgtttctccggaaaaattcccaggtcgttcttctaccaaccatagcatcgtagtgtctggtgacccgcgttttgccggaacgattaaaattaccacgtctgcggttatcgacaaccgtgcgaacctgaactacctgctgtctcactctggtctggactacaaacgtaacattctgaatgaccgtaacccagttgtcactgaagacgttgaaggtgacaaaaaaatctataacgccgaagtcgcggagtgggacaaattgcgccagcgcctgttagacgcacgtaacaaaatcacaagtgctgaatctgcggtcaacagtgcccgcaacaacctgtctgcgcgtaccaacgaacagaaacacgcgaacgacgcgctgaacgcgctgctgaaagaaaaagaaaacatccgtaatcagctttcaggaatcaaccaaaaaattgccgaggaaaagcgtaagcaagacgaactgaaagcgaccaaagacgcgatcaacttcaccaccgagttcctgaaatctgtttctgaaaaatacggtgcgaaggctgagcagcttgcccgcgagatggcggggcaggcgaaaggaaaaaagattcgtaacgttgaagaagcgctgaagacctacgagaaataccgtgcggacatcaacaaaaaaatcaacgcgaaagaccgtgctgcaatcgcggcagctttagaatctgttaaactgtctgacatctcttctaatcttaatcgtttcagtcgtggcttaggttatgcaggcaaattcacctctctggcggactggattactgagttcggcaaagctgttcgcacagaaaattggcgtccccttttcgtaaaaaccgaaaccatcattgcgggcaacgccgcgaccgcgctggttgcgctggttttctctatcctgaccggttctgcgctgggtatcatcggttacggtctgctgatggcggttaccggagcattgattgacgagagtttggtagagaaagccaacaaattctggggtatc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z