BBa_K1962003 1 BBa_K1962003 Colicin Ia::Ssp2 Chimera 2016-10-10T11:00:00Z 2016-10-11T03:58:14Z The sequence encoding Ssp2 was amplified from a Serratia marcescens genomic DNA template and cloned into <partinfo>BBa_K1962002</partinfo>, which had previously been designed and synthesised. This part contains an translational fusion protein of the truncated bacteriocin-free version of Colicin Ia (<partinfo>BBa_K1962002</partinfo>) and an antibacterial toxin (peptidoglycan hydrolase) called Ssp2 from Serratia marcescens. Ssp2 is an antibacterial effector normally secreted by the Type VI secretion system (T7SS) in Serratia marcescens and it has peptidoglycan endopeptidase activity, degrading peptidoglycan in the periplasm of the target cell where it cleaves between y-D-glutamic acid and L-mesodiaminopimelic acid in peptidoglycan. false false _2429_ 8083 8083 9 false Compliant with RFC[10]. false Frank Sargent annotation2491863 1 Ssp2 range2491863 1 1357 1830 annotation2491862 1 Truncated Colicin Ia range2491862 1 1 1350 annotation2491864 1 HA epitope range2491864 1 1831 1863 BBa_K1962003_sequence 1 atgatgtcagacccagtccgcattacgaatcccggcgctgagagcttaggatatgattcggatgggcacgagattatggcagtggacatttatgttaaccccccgcgcgtggacgtttttcacggcacccccccagcttggagcagttttggcaataagacaatctggggtgggaacgaatgggtagacgactcgccaacacgctccgatattgagaaacgtgataaagagattacagcatataagaatacgttatctgcccagcagaaggagaacgagaacaaacgcactgaagcaggcaaacgcctgagtgctgcgatcgcggcacgtgaaaaagacgagaatacgttaaaaactttgcgcgccgggaatgccgatgcggctgacattacgcgtcaagagtttcgtttacttcaagccgaattacgtgaatacggttttcgcacggagatcgcaggatatgatgcattacgcctgcacacagagtcacgcatgttgttcgcggatgctgactcgttacgcatttctccacgtgaagcgcgtagcttaatcgagcaagcggagaagcgtcaaaaagacgctcagaacgctgataagaaagccgcggatatgctggccgaatatgagcgccgtaagggcatccttgacactcgtctttctgaattagaaaaaaacggaggagcagcattagctgttcttgatgctcaacaagctcgtctgttaggacaacagacacgtaacgaccgtgccatctcggaggcgcgtaacaagttgagctcagtaactgagtcgttgaatactgctcgcaacgctttgacccgtgccgagcagcagttgacgcagcaaaagaacactcctgacgggaaaactatcgtttctccggaaaagtttccagggcgcagctcgactaatcactccattgtagtctccggggacccgcgtttcgccgggacgatcaaaatcacaacgagtgccgtcatcgacaaccgtgcaaatctgaactatttactttcccactctggccttgattacaagcgcaacattttaaatgaccgtaaccccgttgttacagaagatgtagaaggtgataagaaaatttacaatgccgaggttgcggagtgggacaagttgcgtcaacgcctgttggatgcacgtaacaaaattacaagcgcagagagtgcagtcaatagtgcccgcaataatttgtccgcacgtacaaatgaacaaaagcacgctaacgatgcattaaacgctctgctgaaggaaaaggaaaacattcgcaaccagctgtcgggcatcaatcaaaagatcgctgaggaaaaacgtaaacaggatgagttaaaggctaccggatccatgagtcgcccttcattccaacaagcgtggcaacgatttagcgaaatcaacgtagatattactgacgttggcaaaaagataggtggaaatgtaggtcttaacattgagcttggcgtcaatgacccattgcaaggattcaccaatgcctgcgcaatacgcatgagttacacgctcaattattctggttttaaagttgaacgaggagcatggaagaccgtttcaggcaaagataaaaattggtacatttatcgcgtcagagatattattaactttttgaattataagttcggcaaacctgataaaacgattaaaaaccctacccctagtgatttttcaaaagacaaagggatattggttttcactgttccaggatggaatgatgccagcggacacgcaacattatggaacggtacaacctgctcagatcattgttactttccaaaatccaacgaggcatctatatggttactaaaatatccatatgatgttccagattatgcagctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z