BBa_K1962007 1 BBa_K1962007 Colicin E9::Ssp1 Chimera 2016-10-10T11:00:00Z 2016-10-11T07:26:58Z The sequence encoding Ssp1 was amplified from a Serratia marcescens genomic DNA template and cloned into <partinfo>BBa_K1962006</partinfo>, which had previously been designed and synthesised. This part contains an translational fusion protein between the truncated DNase-domain-lacking version of Colicin E9 (<partinfo>BBa_K1962006</partinfo>) and an antibacterial toxin (peptidoglycan hydrolase) called Ssp1 from Serratia marcescens. Ssp1 is an antibacterial effector normally secreted by the Type VI secretion system (T6SS) by Serratia marcescens and it has peptidoglycan endopeptidase activity. false false _2429_ 8083 8083 9 false Compliant with RFC[10]. false Frank Sargent annotation2491971 1 Truncated E9 range2491971 1 1 1356 annotation2491973 1 Serratia Ssp1 range2491973 1 1363 1851 annotation2491975 1 HA epitope range2491975 1 1852 1884 BBa_K1962007_sequence 1 atgtctggcggcgacggacgcggccataacactggagcgcatagcacttccgggaacatcaatggggggcccactgggattggcgtctctggcggggcgagtgacggcagtgggtggagttcagaaaacaatccttggggcggcggatcgggatcggggattcattgggggggcggttctggtcgcggtaatggtggaggaaacggtaactccggcggtgggagtggcacagggggtaacttgtcggcagttgcggctcccgtggcgtttggttttcctgctttatcgacaccaggagcaggcgggttggcagttagtatcagcgcctcggaactttctgccgcgattgcaggcattatcgctaagctgaaaaaggttaatttaaagtttacgccgtttggagtggtattatccagcttgatccccagtgagatcgcaaaggatgacccgaatatgatgagcaagatcgtcacttcccttccggccgacgatatcacggaaagccctgtttctagtcttcccttggataaggccaccgtgaatgtcaatgttcgtgttgtagatgacgtcaaagacgaacgccaaaacattagtgtagtcagcggagtccctatgagcgtgcctgttgtagacgccaagcccaccgagcgtccgggtgtctttaccgcgtctattcccggagcacctgtgcttaacattagtgtgaacgactcgaccccagccgtccaaacactttctccgggcgtgacaaataacaccgataaagatgttcgtccagctggttttacccagggcggtaatacccgtgatgctgttattcgttttcccaaagattccggtcacaacgccgtttacgtctcggtgtcagatgtcctttccccagatcaagtcaagcaacgtcaagatgaggaaaaccgccgccaacaagaatgggatgctacacacccggtggaggcagcagaacgtaattacgaacgtgcacgcgccgagttaaaccaggccaatgaggacgtggcgcgcaaccaagaacgccaagccaaggcggtacaggtctacaactcgcgtaagagtgagcttgatgcagcaaacaaaacacttgcagacgccattgcggaaattaaacagtttaatcgcttcgctcatgatccaatggctggtggccatcgtatgtggcagatggctggattgaaagcgcagcgcgctcagactgacgtaaataacaagcaagcggcctttgatgctgccgcgaaagaaaaaagtgacgccgacgcagctctgtcagcggcacaagagcgccgcaagcagaaggagaacaaagaaaaagatgccaaggataaacttgataaggagagtaaaggatccatgaaacctttatatcgtcagctaaaaagcagccattactcctctgattactctagcccgggatatttggcagcagaagccgtttacgctgagattggctatgagcttgatacgcttctaaagcaaaatcctggttatgccaatacctgtgccgtccgcatgagcctggcgctgttgaaaaccggtatcagcttcaaaggccgcctgccaatcaagaaaggcgcctataagggtaaaacgattgaaccgggagccaaattgctggccgatcagttgcatcgctccagtagttttggcaaagccaaaattttctttaatgctccagatgcagagaaaggtattggcaataaaaaaggagtcgtgttcttcaataagatcaccaactatgatggcggacacattgatcttatcgaaccagaaaactcgctattaacctgccattcccattgctattttaactgcaaagaggtctggttttgggagttgagctatccatatgatgttccagattatgcagctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z