BBa_K1962008 1 BBa_K1962008 Colicin E9::Ssp2 Chimera 2016-10-10T11:00:00Z 2016-10-11T07:40:20Z The sequence encoding Ssp2 was amplified from a <i>Serratia marcescens</i> genomic DNA template and cloned into <partinfo>BBa_K1962006</partinfo>, which had previously been designed and synthesised. This part contains an translational fusion protein between the truncated DNase-domain-lacking version of Colicin E9 (<partinfo>BBa_K1962006</partinfo>) and an antibacterial toxin (peptidoglycan hydrolase) called Ssp2 from <i>Serratia marcescens</i>. Ssp2 is an antibacterial effector normally secreted by the Type VI secretion system (T6SS) by <i>Serratia marcescens</i> and it has peptidoglycan endopeptidase activity. false false _2429_ 8083 8083 9 false Compliant with RFC[10] false Frank Sargent annotation2491986 1 Truncated E9 range2491986 1 1 1356 annotation2491987 1 Serratia Ssp2 range2491987 1 1363 1836 annotation2491988 1 HA epitope range2491988 1 1837 1869 BBa_K1962008_sequence 1 atgtctggcggcgacggacgcggccataacactggagcgcatagcacttccgggaacatcaatggggggcccactgggattggcgtctctggcggggcgagtgacggcagtgggtggagttcagaaaacaatccttggggcggcggatcgggatcggggattcattgggggggcggttctggtcgcggtaatggtggaggaaacggtaactccggcggtgggagtggcacagggggtaacttgtcggcagttgcggctcccgtggcgtttggttttcctgctttatcgacaccaggagcaggcgggttggcagttagtatcagcgcctcggaactttctgccgcgattgcaggcattatcgctaagctgaaaaaggttaatttaaagtttacgccgtttggagtggtattatccagcttgatccccagtgagatcgcaaaggatgacccgaatatgatgagcaagatcgtcacttcccttccggccgacgatatcacggaaagccctgtttctagtcttcccttggataaggccaccgtgaatgtcaatgttcgtgttgtagatgacgtcaaagacgaacgccaaaacattagtgtagtcagcggagtccctatgagcgtgcctgttgtagacgccaagcccaccgagcgtccgggtgtctttaccgcgtctattcccggagcacctgtgcttaacattagtgtgaacgactcgaccccagccgtccaaacactttctccgggcgtgacaaataacaccgataaagatgttcgtccagctggttttacccagggcggtaatacccgtgatgctgttattcgttttcccaaagattccggtcacaacgccgtttacgtctcggtgtcagatgtcctttccccagatcaagtcaagcaacgtcaagatgaggaaaaccgccgccaacaagaatgggatgctacacacccggtggaggcagcagaacgtaattacgaacgtgcacgcgccgagttaaaccaggccaatgaggacgtggcgcgcaaccaagaacgccaagccaaggcggtacaggtctacaactcgcgtaagagtgagcttgatgcagcaaacaaaacacttgcagacgccattgcggaaattaaacagtttaatcgcttcgctcatgatccaatggctggtggccatcgtatgtggcagatggctggattgaaagcgcagcgcgctcagactgacgtaaataacaagcaagcggcctttgatgctgccgcgaaagaaaaaagtgacgccgacgcagctctgtcagcggcacaagagcgccgcaagcagaaggagaacaaagaaaaagatgccaaggataaacttgataaggagagtaaaggatccatgagtcgcccttcattccaacaagcgtggcaacgatttagcgaaatcaacgtagatattactgacgttggcaaaaagataggtggaaatgtaggtcttaacattgagcttggcgtcaatgacccattgcaaggattcaccaatgcctgcgcaatacgcatgagttacacgctcaattattctggttttaaagttgaacgaggagcatggaagaccgtttcaggcaaagataaaaattggtacatttatcgcgtcagagatattattaactttttgaattataagttcggcaaacctgataaaacgattaaaaaccctacccctagtgatttttcaaaagacaaagggatattggttttcactgttccaggatggaatgatgccagcggacacgcaacattatggaacggtacaacctgctcagatcattgttactttccaaaatccaacgaggcatctatatggttactaaaatatccatatgatgttccagattatgcagctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z