LOCUS       BBa_K1962014            1026 bp    DNA     linear       10-Jun-2024
DEFINITION  A pH Sensing Device Based on The Asr Promoter
ACCESSION   BBa_K1962014
VERSION     BBa_K1962014.1  
FEATURES             Location/Qualifiers
     promoter        1..140
                     /label=BBa_K1231000
     RBS             149..163
                     /label=RBS-1\Strong
     RBS             156..160
                     /label=RBS
     misc_feature    149..163
                     /label=BBa_B0030
     RBS             149..163
                     /label=BBa_B0030
     CDS             170..889
                     /label=GFP protein
     CDS             170..889
                     /label=BBa_E0040
     stem_loop       909..952
                     /label=stem_loop
     misc_feature    898..977
                     /label=BBa_B0010
     terminator      898..977
                     /label=BBa_B0010
     stem_loop       993..1012
                     /label=T7 TE
     misc_feature    1019..1019
                     /label=stop
     polyA_site      1013..1026
                     /label=polya
     misc_feature    986..1026
                     /label=BBa_B0012
     terminator      986..1026
                     /label=BBa_B0012
     misc_feature    149..1026
                     /label=BBa_E0840
ORIGIN
        1 cgctgtaatt tattcagcgt ttgtacatat cgttacacgc tgaaaccaac cactcacgga
       61 agtctgccat tcccagggat atagttattt caacggcccc gcagtggggt taaatgaaaa
      121 aacaaattga gggtatgaca tactagagat taaagaggag aaatactaga tgcgtaaagg
      181 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      241 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      301 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      361 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      421 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      481 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      541 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      601 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
      661 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
      721 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
      781 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
      841 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcca
      901 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      961 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1021 tttata
//